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Infect Immun. Nov 1995; 63(11): 4295–4300.
PMCID: PMC173610

Campylobacter jejuni motility and invasion of Caco-2 cells.

Abstract

We investigated the influence of motility on Campylobacter jejuni binding and invasion of Caco-2 cells. C. jejuni was motile in soft agar at basic (pH 8.5) and neutral pH values representative of the intestinal environment. However, C. jejuni was immobilized at pH 5.0. The inability of C. jejuni to swarm on soft agar at pH 5.0 was not related to flagellar depolymerization or loss of viability. In tissue culture medium, C. jejuni displayed typical periods of straight swimming punctuated by tumbling behavior. This behavior was altered when the viscosity of the medium was adjusted to mimic the viscosity of intestinal mucus. C. jejuni showed longer periods of straight swimming with significantly increased velocity followed by pauses instead of tumbles. The binding and invasion of C. jejuni in Caco-2 cells also increased significantly in high-viscosity growth medium. We speculate that the swimming behavior of C. jejuni in a viscous environment may be an important factor in the interaction of these organisms with host epithelial cells. The pH, which affects C. jejuni motility, may also influence the tropism of these organisms.

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Selected References

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  • Alm RA, Guerry P, Trust TJ. The Campylobacter sigma 54 flaB flagellin promoter is subject to environmental regulation. J Bacteriol. 1993 Jul;175(14):4448–4455. [PMC free article] [PubMed]
  • Alm RA, Guerry P, Trust TJ. Distribution and polymorphism of the flagellin genes from isolates of Campylobacter coli and Campylobacter jejuni. J Bacteriol. 1993 May;175(10):3051–3057. [PMC free article] [PubMed]
  • Babakhani FK, Bradley GA, Joens LA. Newborn piglet model for campylobacteriosis. Infect Immun. 1993 Aug;61(8):3466–3475. [PMC free article] [PubMed]
  • Berg HC, Turner L. Movement of microorganisms in viscous environments. Nature. 1979 Mar 22;278(5702):349–351. [PubMed]
  • Bukholm G, Kapperud G. Expression of Campylobacter jejuni invasiveness in cell cultures coinfected with other bacteria. Infect Immun. 1987 Nov;55(11):2816–2821. [PMC free article] [PubMed]
  • De Melo MA, Gabbiani G, Pechère JC. Cellular events and intracellular survival of Campylobacter jejuni during infection of HEp-2 cells. Infect Immun. 1989 Jul;57(7):2214–2222. [PMC free article] [PubMed]
  • de Melo MA, Pechère JC. Effect of mucin on Campylobacter jejuni association and invasion on HEp-2 cells. Microb Pathog. 1988 Jul;5(1):71–76. [PubMed]
  • Ferrero RL, Lee A. Motility of Campylobacter jejuni in a viscous environment: comparison with conventional rod-shaped bacteria. J Gen Microbiol. 1988 Jan;134(1):53–59. [PubMed]
  • Greenberg EP, Canale-Parola E. Motility of flagellated bacteria in viscous environments. J Bacteriol. 1977 Oct;132(1):356–358. [PMC free article] [PubMed]
  • HOLWILL ME, BURGE RE. A hydrodynamic study of the motility of flagellated bacteria. Arch Biochem Biophys. 1963 May;101:249–260. [PubMed]
  • King M. Viscoelastic properties of airway mucus. Fed Proc. 1980 Nov;39(13):3080–3085. [PubMed]
  • Konkel ME, Joens LA. Adhesion to and invasion of HEp-2 cells by Campylobacter spp. Infect Immun. 1989 Oct;57(10):2984–2990. [PMC free article] [PubMed]
  • Konkel ME, Joens LA. Effect of enteroviruses on adherence to and invasion of HEp-2 cells by Campylobacter isolates. Infect Immun. 1990 Apr;58(4):1101–1105. [PMC free article] [PubMed]
  • Konkel ME, Mead DJ, Hayes SF, Cieplak W., Jr Translocation of Campylobacter jejuni across human polarized epithelial cell monolayer cultures. J Infect Dis. 1992 Aug;166(2):308–315. [PubMed]
  • Lee A, O'Rourke JL, Barrington PJ, Trust TJ. Mucus colonization as a determinant of pathogenicity in intestinal infection by Campylobacter jejuni: a mouse cecal model. Infect Immun. 1986 Feb;51(2):536–546. [PMC free article] [PubMed]
  • Mantle M, Stewart G, Zayas G, King M. The disulphide-bond content and rheological properties of intestinal mucins from normal subjects and patients with cystic fibrosis. Biochem J. 1990 Mar 1;266(2):597–604. [PMC free article] [PubMed]
  • McSweegan E, Burr DH, Walker RI. Intestinal mucus gel and secretory antibody are barriers to Campylobacter jejuni adherence to INT 407 cells. Infect Immun. 1987 Jun;55(6):1431–1435. [PMC free article] [PubMed]
  • McSweegan E, Walker RI. Identification and characterization of two Campylobacter jejuni adhesins for cellular and mucous substrates. Infect Immun. 1986 Jul;53(1):141–148. [PMC free article] [PubMed]
  • Newell DG, Pearson A. The invasion of epithelial cell lines and the intestinal epithelium of infant mice by Campylobacter jejuni/coli. J Diarrhoeal Dis Res. 1984 Mar;2(1):19–26. [PubMed]
  • Ruiz-Palacios GM, Escamilla E, Torres N. Experimental Campylobacter diarrhea in chickens. Infect Immun. 1981 Oct;34(1):250–255. [PMC free article] [PubMed]
  • Russell RG, Blake DC., Jr Cell association and invasion of Caco-2 cells by Campylobacter jejuni. Infect Immun. 1994 Sep;62(9):3773–3779. [PMC free article] [PubMed]
  • Russell RG, O'Donnoghue M, Blake DC, Jr, Zulty J, DeTolla LJ. Early colonic damage and invasion of Campylobacter jejuni in experimentally challenged infant Macaca mulatta. J Infect Dis. 1993 Jul;168(1):210–215. [PubMed]
  • Sanyal SC, Islam KM, Neogy PK, Islam M, Speelman P, Huq MI. Campylobacter jejuni diarrhea model in infant chickens. Infect Immun. 1984 Mar;43(3):931–936. [PMC free article] [PubMed]
  • Schneider WR, Doetsch RN. Effect of viscosity on bacterial motility. J Bacteriol. 1974 Feb;117(2):696–701. [PMC free article] [PubMed]
  • van Spreeuwel JP, Duursma GC, Meijer CJ, Bax R, Rosekrans PC, Lindeman J. Campylobacter colitis: histological immunohistochemical and ultrastructural findings. Gut. 1985 Sep;26(9):945–951. [PMC free article] [PubMed]
  • Wassenaar TM, Bleumink-Pluym NM, van der Zeijst BA. Inactivation of Campylobacter jejuni flagellin genes by homologous recombination demonstrates that flaA but not flaB is required for invasion. EMBO J. 1991 Aug;10(8):2055–2061. [PMC free article] [PubMed]
  • Welkos SL. Experimental gastroenteritis in newly-hatched chicks infected with Campylobacter jejuni. J Med Microbiol. 1984 Oct;18(2):233–248. [PubMed]
  • Yanagawa Y, Takahashi M, Itoh T. [The role of flagella of Campylobacter jejuni in colonization in the intestinal tract in mice and the cultured-cell infectivity]. Nihon Saikingaku Zasshi. 1994 Mar;49(2):395–403. [PubMed]
  • Yao R, Burr DH, Doig P, Trust TJ, Niu H, Guerry P. Isolation of motile and non-motile insertional mutants of Campylobacter jejuni: the role of motility in adherence and invasion of eukaryotic cells. Mol Microbiol. 1994 Dec;14(5):883–893. [PubMed]

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