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Infect Immun. Oct 1995; 63(10): 4150–4153.
PMCID: PMC173583

Interaction of laminin with Entamoeba histolytica cysteine proteinases and its effect on amebic pathogenesis.


The Entamoeba histolytica 27-kDa cysteine proteinases exhibit striking binding specificities for immobilized laminin over other components of the extracellular matrix, such as collagen and fibronectin. Inactivation of the proteinase with the active-site inhibitor L-trans-epoxysuccinyl-leucylamido(4-guanidino)butane abolishes laminin binding by the enzyme, and conversely, laminin inhibits cleavage of a fluorogenic dipeptide substrate of the amebic cysteine proteinase, suggesting that the substrate binding pocket of the enzyme is involved in the binding of laminin. The addition of laminin but not fibronectin or collagen to E. histolytica trophozoites significantly reduces amebic liver abscess formation in severe combined immunodeficient mice, further supporting the hypothesis that E. histolytica cysteine proteinases play an important role in amebic pathogenesis. The specific interaction of amebic proteinases with laminin may be exploited in designing new inhibitors of these enzymes.

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Selected References

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  • Barrett AJ, Kembhavi AA, Brown MA, Kirschke H, Knight CG, Tamai M, Hanada K. L-trans-Epoxysuccinyl-leucylamido(4-guanidino)butane (E-64) and its analogues as inhibitors of cysteine proteinases including cathepsins B, H and L. Biochem J. 1982 Jan 1;201(1):189–198. [PMC free article] [PubMed]
  • Beck K, Hunter I, Engel J. Structure and function of laminin: anatomy of a multidomain glycoprotein. FASEB J. 1990 Feb 1;4(2):148–160. [PubMed]
  • Diamond LS, Harlow DR, Cunnick CC. A new medium for the axenic cultivation of Entamoeba histolytica and other Entamoeba. Trans R Soc Trop Med Hyg. 1978;72(4):431–432. [PubMed]
  • Gadasi H, Kobiler D. Entamoeba histolytica: correlation between virulence and content of proteolytic enzymes. Exp Parasitol. 1983 Feb;55(1):105–110. [PubMed]
  • Guo NH, Krutzsch HC, Vogel T, Roberts DD. Interactions of a laminin-binding peptide from a 33-kDa protein related to the 67-kDa laminin receptor with laminin and melanoma cells are heparin-dependent. J Biol Chem. 1992 Sep 5;267(25):17743–17747. [PubMed]
  • Keene WE, Petitt MG, Allen S, McKerrow JH. The major neutral proteinase of Entamoeba histolytica. J Exp Med. 1986 Mar 1;163(3):536–549. [PMC free article] [PubMed]
  • Luaces AL, Barrett AJ. Affinity purification and biochemical characterization of histolysin, the major cysteine proteinase of Entamoeba histolytica. Biochem J. 1988 Mar 15;250(3):903–909. [PMC free article] [PubMed]
  • Martínez-Palomo A. The pathogenesis of amoebiasis. Parasitol Today. 1987 Apr;3(4):111–118. [PubMed]
  • Matsudaira P. Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J Biol Chem. 1987 Jul 25;262(21):10035–10038. [PubMed]
  • Mecham RP. Laminin receptors. Annu Rev Cell Biol. 1991;7:71–91. [PubMed]
  • Pérez-Tamayo R, Becker I, Montfort I, Ostoa-Saloma P, Pérez-Montfort R. Role of leukocytes and amebic proteinases in experimental rat testicular necrosis produced by Entamoeba histolytica. Parasitol Res. 1991;77(3):192–196. [PubMed]
  • Reed SL, Keene WE, McKerrow JH. Thiol proteinase expression and pathogenicity of Entamoeba histolytica. J Clin Microbiol. 1989 Dec;27(12):2772–2777. [PMC free article] [PubMed]
  • Schulte W, Scholze H. Action of the major protease from Entamoeba histolytica on proteins of the extracellular matrix. J Protozool. 1989 Nov-Dec;36(6):538–543. [PubMed]
  • Stanley SL, Jr, Zhang T, Rubin D, Li E. Role of the Entamoeba histolytica cysteine proteinase in amebic liver abscess formation in severe combined immunodeficient mice. Infect Immun. 1995 Apr;63(4):1587–1590. [PMC free article] [PubMed]
  • Stochaj U, Mannherz HG. Chicken gizzard 5'-nucleotidase functions as a binding protein for the laminin/nidogen complex. Eur J Cell Biol. 1992 Dec;59(2):364–372. [PubMed]
  • Takeuchi A, Phillips BP. Electron microscope studies of experimental Entamoeba histolytica infection in the guinea pig. I. Penetration of the intestinal epithelium by trophozoites. Am J Trop Med Hyg. 1975 Jan;24(1):34–48. [PubMed]
  • Tannich E, Nickel R, Buss H, Horstmann RD. Mapping and partial sequencing of the genes coding for two different cysteine proteinases in pathogenic Entamoeba histolytica. Mol Biochem Parasitol. 1992 Aug;54(1):109–111. [PubMed]
  • Varughese KI, Ahmed FR, Carey PR, Hasnain S, Huber CP, Storer AC. Crystal structure of a papain-E-64 complex. Biochemistry. 1989 Feb 7;28(3):1330–1332. [PubMed]
  • Vicentini AP, Gesztesi JL, Franco MF, de Souza W, de Moraes JZ, Travassos LR, Lopes JD. Binding of Paracoccidioides brasiliensis to laminin through surface glycoprotein gp43 leads to enhancement of fungal pathogenesis. Infect Immun. 1994 Apr;62(4):1465–1469. [PMC free article] [PubMed]
  • Woo HJ, Shaw LM, Messier JM, Mercurio AM. The major non-integrin laminin binding protein of macrophages is identical to carbohydrate binding protein 35 (Mac-2). J Biol Chem. 1990 May 5;265(13):7097–7099. [PubMed]
  • Zhou Q, Cummings RD. The S-type lectin from calf heart tissue binds selectively to the carbohydrate chains of laminin. Arch Biochem Biophys. 1990 Aug 15;281(1):27–35. [PubMed]

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