Logo of jmedgeneJournal of Medical GeneticsVisit this articleSubmit a manuscriptReceive email alertsContact usBMJ
J Med Genet. 2003 Sep; 40(9): 664–670.
PMCID: PMC1735585

Array painting: a method for the rapid analysis of aberrant chromosomes using DNA microarrays


Objective: The authors describe a method, termed array painting, which allows the rapid, high resolution analysis of the content and breakpoints of aberrant chromosomes.

Methods: Array painting is similar in concept to reverse chromosome painting and involves the hybridisation of probes generated by PCR of small numbers of flow sorted chromosomes on large insert genomic clone DNA microarrays.

Results and Conclusions: By analysing patients with cytogenetically balanced chromosome rearrangements, the authors show the effectiveness of array painting as a method to map breakpoints prior to cloning and sequencing chromosome rearrangements.

Full Text

The Full Text of this article is available as a PDF (754K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Solinas-Toldo S, Lampel S, Stilgenbauer S, Nickolenko J, Benner A, Döhner H, Cremer T, Lichter P. Matrix-based comparative genomic hybridization: biochips to screen for genomic imbalances. Genes Chromosomes Cancer. 1997 Dec;20(4):399–407. [PubMed]
  • Pinkel D, Segraves R, Sudar D, Clark S, Poole I, Kowbel D, Collins C, Kuo WL, Chen C, Zhai Y, et al. High resolution analysis of DNA copy number variation using comparative genomic hybridization to microarrays. Nat Genet. 1998 Oct;20(2):207–211. [PubMed]
  • Fiegler Heike, Carr Philippa, Douglas Eleanor J, Burford Deborah C, Hunt Sarah, Scott Carol E, Smith James, Vetrie David, Gorman Patricia, Tomlinson Ian P M, et al. DNA microarrays for comparative genomic hybridization based on DOP-PCR amplification of BAC and PAC clones. Genes Chromosomes Cancer. 2003 Apr;36(4):361–374. [PubMed]
  • Cai Wei-Wen, Mao Jian-Hua, Chow Chi-Wen, Damani Shamsha, Balmain Allan, Bradley Allan. Genome-wide detection of chromosomal imbalances in tumors using BAC microarrays. Nat Biotechnol. 2002 Apr;20(4):393–396. [PubMed]
  • Carter NP, Ferguson-Smith MA, Perryman MT, Telenius H, Pelmear AH, Leversha MA, Glancy MT, Wood SL, Cook K, Dyson HM, et al. Reverse chromosome painting: a method for the rapid analysis of aberrant chromosomes in clinical cytogenetics. J Med Genet. 1992 May;29(5):299–307. [PMC free article] [PubMed]
  • Carter NP. Bivariate chromosome analysis using a commercial flow cytometer. Methods Mol Biol. 1994;29:187–204. [PubMed]
  • McMullan Tristan W, Crolla John A, Gregory Simon G, Carter Nigel P, Cooper Rachel A, Howell Gareth R, Robinson David O. A candidate gene for congenital bilateral isolated ptosis identified by molecular analysis of a de novo balanced translocation. Hum Genet. 2002 Mar;110(3):244–250. [PubMed]

Articles from Journal of Medical Genetics are provided here courtesy of BMJ Group


Save items

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


  • Cited in Books
    Cited in Books
    PubMed Central articles cited in books
  • MedGen
    Related information in MedGen
  • PubMed
    PubMed citations for these articles

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...