• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of iaiPermissionsJournals.ASM.orgJournalIAI ArticleJournal InfoAuthorsReviewers
Infect Immun. Sep 1995; 63(9): 3348–3352.
PMCID: PMC173460

Structural characterization of the lipopolysaccharide O antigens of Burkholderia pseudomallei.

Abstract

A serologically typical strain of Burkholderia pseudomallei (strain 304b) was found to produce two S-type lipopolysaccharides (LPS) differing in the chemical structures of their O-polysaccharide (O-PS) components. Structural analysis revealed that one O-antigenic polysaccharide (O-PS I) is an unbranched high-molecular-weight polymer of 1,3-linked 2-O-acetyl-6-deoxy-beta-D-manno-heptopyranose residues. The other LPS O antigen (O-PS II) is an unbranched polymer of repeating disaccharide units having the structure -3)-beta-D-glucopyranose-(1-3)-6-deoxy-alpha-L-talopyranose-(1- in which ca. 33% of the L-6dTalp residues bear 2-O-methyl and 4-O-acetyl substituents while the other L-6dTalp residues carry only 2-O-acetyl substituents. Analysis of a serologically atypical strain of B. pseudomallei (strain 824a) produced a single LPS O-PS which was chemically identical to the 6-deoxy-D-manno-hepan O-PS I. The production of two distinct LPS raises the interesting question of their relative immunogenicities and consequently their relative importance for diagnostic serology and for the possible development of conjugate vaccines.

Full Text

The Full Text of this article is available as a PDF (203K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • AMINOFF D. Methods for the quantitative estimation of N-acetylneuraminic acid and their application to hydrolysates of sialomucoids. Biochem J. 1961 Nov;81:384–392. [PMC free article] [PubMed]
  • Brett PJ, Mah DC, Woods DE. Isolation and characterization of Pseudomonas pseudomallei flagellin proteins. Infect Immun. 1994 May;62(5):1914–1919. [PMC free article] [PubMed]
  • Beynon LM, Perry MB. Structure of the lipopolysaccharide O-antigen of Pseudomonas cepacia serotype A. Biochem Cell Biol. 1993 Sep-Oct;71(9-10):417–420. [PubMed]
  • Brisson JR, Perry MB. The structures of the two lipopolysaccharide O-chains produced by Salmonella boecker. Biochem Cell Biol. 1988 Oct;66(10):1066–1077. [PubMed]
  • Chaowagul W, White NJ, Dance DA, Wattanagoon Y, Naigowit P, Davis TM, Looareesuwan S, Pitakwatchara N. Melioidosis: a major cause of community-acquired septicemia in northeastern Thailand. J Infect Dis. 1989 May;159(5):890–899. [PubMed]
  • Dance DA. Melioidosis: the tip of the iceberg? Clin Microbiol Rev. 1991 Jan;4(1):52–60. [PMC free article] [PubMed]
  • Di Fabio JL, Brisson JR, Perry MB. Structural analysis of the three lipopolysaccharides produced by Salmonella madelia (1,6,14,25). Biochem Cell Biol. 1989 Feb-Mar;67(2-3):78–85. [PubMed]
  • Gatt R, Berman ER. A rapid procedure for the estimation of amino sugars on a micro scale. Anal Biochem. 1966 Apr;15(1):167–171. [PubMed]
  • HAKOMORI S. A RAPID PERMETHYLATION OF GLYCOLIPID, AND POLYSACCHARIDE CATALYZED BY METHYLSULFINYL CARBANION IN DIMETHYL SULFOXIDE. J Biochem. 1964 Feb;55:205–208. [PubMed]
  • Johnson KG, Perry MB. Improved techniques for the preparation of bacterial lipopolysaccharides. Can J Microbiol. 1976 Jan;22(1):29–34. [PubMed]
  • Knirel YA, Paramonov NA, Shashkov AS, Kochetkov NK, Yarullin RG, Farber SM, Efremenko VI. Structure of the polysaccharide chains of Pseudomonas pseudomallei lipopolysaccharides. Carbohydr Res. 1992 Sep 2;233:185–193. [PubMed]
  • Leelarasamee A, Bovornkitti S. Melioidosis: review and update. Rev Infect Dis. 1989 May-Jun;11(3):413–425. [PubMed]
  • MARKOVITZ A. Isolation of D-talomethylose (6-deoxy-Dtalose) and D-rhamnose (6-deoxy-D-mannose) from capsular polysaccharide of a gram-negative bacterium. J Biol Chem. 1962 Jun;237:1767–1771. [PubMed]
  • Pitt TL, Aucken H, Dance DA. Homogeneity of lipopolysaccharide antigens in Pseudomonas pseudomallei. J Infect. 1992 Sep;25(2):139–146. [PubMed]
  • Yabuuchi E, Kosako Y, Oyaizu H, Yano I, Hotta H, Hashimoto Y, Ezaki T, Arakawa M. Proposal of Burkholderia gen. nov. and transfer of seven species of the genus Pseudomonas homology group II to the new genus, with the type species Burkholderia cepacia (Palleroni and Holmes 1981) comb. nov. Microbiol Immunol. 1992;36(12):1251–1275. [PubMed]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...