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Infect Immun. Jan 1995; 63(1): 356–359.
PMCID: PMC173001

Rapid disruption of epithelial barrier function by Salmonella typhimurium is associated with structural modification of intercellular junctions.

Abstract

Short-term infection of MDCK II monolayers with Salmonella typhimurium SL1344 caused a progressive decrease in transepithelial electrical resistance concomitant with decreased cation permselectivity and increased paracellular inulin flux. Cytochemical staining of F-actin, E-cadherin, and ZO-1 revealed the concentration of each junctional protein in invaded cells as a result of contraction at their apical poles and resultant distortion of adjacent uninvaded cells.

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Selected References

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  • Barker G, Simmons NL. Identification of two strains of cultured canine renal epithelial cells (MDCK cells) which display entirely different physiological properties. Q J Exp Physiol. 1981 Jan;66(1):61–72. [PubMed]
  • Canil C, Rosenshine I, Ruschkowski S, Donnenberg MS, Kaper JB, Finlay BB. Enteropathogenic Escherichia coli decreases the transepithelial electrical resistance of polarized epithelial monolayers. Infect Immun. 1993 Jul;61(7):2755–2762. [PMC free article] [PubMed]
  • Citi S. Protein kinase inhibitors prevent junction dissociation induced by low extracellular calcium in MDCK epithelial cells. J Cell Biol. 1992 Apr;117(1):169–178. [PMC free article] [PubMed]
  • Clark MA, Jepson MA, Simmons NL, Hirst BH. Preferential interaction of Salmonella typhimurium with mouse Peyer's patch M cells. Res Microbiol. 1994 Sep;145(7):543–552. [PubMed]
  • Collares-Buzato CB, Jepson MA, McEwan GT, Simmons NL, Hirst BH. Junctional uvomorulin/E-cadherin and phosphotyrosine-modified protein content are correlated with paracellular permeability in Madin-Darby canine kidney (MDCK) epithelia. Histochemistry. 1994 Mar;101(3):185–194. [PubMed]
  • Collares-Buzato CB, McEwan GT, Jepson MA, Simmons NL, Hirst BH. Paracellular barrier and junctional protein distribution depend on basolateral extracellular Ca2+ in cultured epithelia. Biochim Biophys Acta. 1994 Jun 30;1222(2):147–158. [PubMed]
  • Finlay BB, Falkow S. Salmonella interactions with polarized human intestinal Caco-2 epithelial cells. J Infect Dis. 1990 Nov;162(5):1096–1106. [PubMed]
  • Finlay BB, Gumbiner B, Falkow S. Penetration of Salmonella through a polarized Madin-Darby canine kidney epithelial cell monolayer. J Cell Biol. 1988 Jul;107(1):221–230. [PMC free article] [PubMed]
  • Francis CL, Starnbach MN, Falkow S. Morphological and cytoskeletal changes in epithelial cells occur immediately upon interaction with Salmonella typhimurium grown under low-oxygen conditions. Mol Microbiol. 1992 Nov;6(21):3077–3087. [PubMed]
  • Galán JE. Salmonella entry into mammalian cells: different yet converging signal transduction pathways? Trends Cell Biol. 1994 Jun;4(6):196–199. [PubMed]
  • Galán JE, Pace J, Hayman MJ. Involvement of the epidermal growth factor receptor in the invasion of cultured mammalian cells by Salmonella typhimurium. Nature. 1992 Jun 18;357(6379):588–589. [PubMed]
  • Ginocchio C, Pace J, Galán JE. Identification and molecular characterization of a Salmonella typhimurium gene involved in triggering the internalization of salmonellae into cultured epithelial cells. Proc Natl Acad Sci U S A. 1992 Jul 1;89(13):5976–5980. [PMC free article] [PubMed]
  • Jones BD, Paterson HF, Hall A, Falkow S. Salmonella typhimurium induces membrane ruffling by a growth factor-receptor-independent mechanism. Proc Natl Acad Sci U S A. 1993 Nov 1;90(21):10390–10394. [PMC free article] [PubMed]
  • McEwan GT, Jepson MA, Hirst BH, Simmons NL. Polycation-induced enhancement of epithelial paracellular permeability is independent of tight junctional characteristics. Biochim Biophys Acta. 1993 May 14;1148(1):51–60. [PubMed]
  • Pace J, Hayman MJ, Galán JE. Signal transduction and invasion of epithelial cells by S. typhimurium. Cell. 1993 Feb 26;72(4):505–514. [PubMed]
  • Peterson MW, Gruenhaupt D. A23187 increases permeability of MDCK monolayers independent of phospholipase activation. Am J Physiol. 1990 Jul;259(1 Pt 1):C69–C76. [PubMed]
  • Ruschkowski S, Rosenshine I, Finlay BB. Salmonella typhimurium induces an inositol phosphate flux in infected epithelial cells. FEMS Microbiol Lett. 1992 Aug 15;74(2-3):121–126. [PubMed]
  • Shasby DM, Winter M, Shasby SS. Oxidants and conductance of cultured epithelial cell monolayers: inositol phospholipid hydrolysis. Am J Physiol. 1988 Dec;255(6 Pt 1):C781–C788. [PubMed]
  • Takeuchi A. Electron microscope studies of experimental Salmonella infection. I. Penetration into the intestinal epithelium by Salmonella typhimurium. Am J Pathol. 1967 Jan;50(1):109–136. [PMC free article] [PubMed]

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