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Clin Microbiol Rev. Jan 1997; 10(1): 160–184.
PMCID: PMC172947

Current methods of laboratory diagnosis of Chlamydia trachomatis infections.

Abstract

Infections caused by Chlamydia trachomatis are probably the most common sexually transmitted diseases in the United States. Commonly unrecognized and often inadequately treated, chlamydial infections can ascend the reproductive tract and cause pelvic inflammatory disease, which often results in the devastating consequences of infertility, ectopic pregnancy, or chronic pelvic pain. C. trachomatis infections are also known to increase the risk for human immunodeficiency virus infection. The obligate intracellular life cycle of C. trachomatis has traditionally required laboratory diagnostic tests that are technically demanding, labor-intensive, expensive, and difficult to access. In spite of these historical challenges, however, laboratory diagnosis of C. trachomatis has been a rapidly advancing area in which there is presently a wide array of commercial diagnostic technologies, costs, manufacturers. This review describes and compares the diagnostic methods for C. trachomatis infection that are currently approved for use in the United States, including the newest DNA amplification technologies which are yet to be licensed for commercial use. Issues to consider in selecting a test for purposes of screening versus diagnosis based on prevalence, performance, legal, social, and cost issues are also discussed.

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Selected References

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  • Akane A, Matsubara K, Nakamura H, Takahashi S, Kimura K. Identification of the heme compound copurified with deoxyribonucleic acid (DNA) from bloodstains, a major inhibitor of polymerase chain reaction (PCR) amplification. J Forensic Sci. 1994 Mar;39(2):362–372. [PubMed]
  • Altaie SS, Meier FA, Centor RM, Wakabongo M, Toksoz D, Harvey KM, Basinger E, Johnson BA, Brookman RR, Dalton HP. Evaluation of two ELISA's for detecting Chlamydia trachomatis from endocervical swabs. Diagn Microbiol Infect Dis. 1992 Sep-Oct;15(7):579–586. [PubMed]
  • An Q, Liu J, O'Brien W, Radcliffe G, Buxton D, Popoff S, King W, Vera-Garcia M, Lu L, Shah J, et al. Comparison of characteristics of Q beta replicase-amplified assay with competitive PCR assay for Chlamydia trachomatis. J Clin Microbiol. 1995 Jan;33(1):58–63. [PMC free article] [PubMed]
  • An Q, Radcliffe G, Vassallo R, Buxton D, O'Brien WJ, Pelletier DA, Weisburg WG, Klinger JD, Olive DM. Infection with a plasmid-free variant Chlamydia related to Chlamydia trachomatis identified by using multiple assays for nucleic acid detection. J Clin Microbiol. 1992 Nov;30(11):2814–2821. [PMC free article] [PubMed]
  • Anderson JR, Mumtaz G, Mulhare P, Poddar J, Ridgway GL. Mandatory use of confirmation stage with Chlamydiazyme during urinary sediment analysis. J Clin Pathol. 1993 Oct;46(10):896–897. [PMC free article] [PubMed]
  • Bäckman M, Rudén AK, Ringertz O, Sandström EG. Detection of Chlamydia trachomatis in urine from men with urethritis. Eur J Clin Microbiol Infect Dis. 1993 Jun;12(6):447–449. [PubMed]
  • Barnes RC. Laboratory diagnosis of human chlamydial infections. Clin Microbiol Rev. 1989 Apr;2(2):119–136. [PMC free article] [PubMed]
  • Bass CA, Jungkind DL, Silverman NS, Bondi JM. Clinical evaluation of a new polymerase chain reaction assay for detection of Chlamydia trachomatis in endocervical specimens. J Clin Microbiol. 1993 Oct;31(10):2648–2653. [PMC free article] [PubMed]
  • Bassiri M, Hu HY, Domeika MA, Burczak J, Svensson LO, Lee HH, Mårdh PA. Detection of Chlamydia trachomatis in urine specimens from women by ligase chain reaction. J Clin Microbiol. 1995 Apr;33(4):898–900. [PMC free article] [PubMed]
  • Batteiger BE, Jones RB. Chlamydial infections. Infect Dis Clin North Am. 1987 Mar;1(1):55–81. [PubMed]
  • Bauwens JE, Clark AM, Loeffelholz MJ, Herman SA, Stamm WE. Diagnosis of Chlamydia trachomatis urethritis in men by polymerase chain reaction assay of first-catch urine. J Clin Microbiol. 1993 Nov;31(11):3013–3016. [PMC free article] [PubMed]
  • Bauwens JE, Clark AM, Stamm WE. Diagnosis of Chlamydia trachomatis endocervical infections by a commercial polymerase chain reaction assay. J Clin Microbiol. 1993 Nov;31(11):3023–3027. [PMC free article] [PubMed]
  • Beebe JL, Rau MP, Albrecht KD. Confirmatory testing of Chlamydia trachomatis Syva enzyme immunoassay gray zone specimens by Syva direct fluorescent antibody test. Sex Transm Dis. 1993 May-Jun;20(3):140–142. [PubMed]
  • Bell TA, Kuo CC, Stamm WE, Tam MR, Stephens RS, Holmes KK, Grayston JT. Direct fluorescent monoclonal antibody stain for rapid detection of infant Chlamydia trachomatis infections. Pediatrics. 1984 Aug;74(2):224–228. [PubMed]
  • Bell TA, Stamm WE, Kuo CC, Wang SP, Holmes KK, Grayston JT. Delayed appearance of Chlamydia trachomatis infections acquired at birth. Pediatr Infect Dis J. 1987 Oct;6(10):928–931. [PubMed]
  • Berg AO, Heidrich FE, Fihn SD, Bergman JJ, Wood RW, Stamm WE, Holmes KK. Establishing the cause of genitourinary symptoms in women in a family practice. Comparison of clinical examination and comprehensive microbiology. JAMA. 1984 Feb 3;251(5):620–625. [PubMed]
  • Berger RE, Alexander ER, Harnisch JP, Paulsen CA, Monda GD, Ansell J, Holmes KK. Etiology, manifestations and therapy of acute epididymitis: prospective study of 50 cases. J Urol. 1979 Jun;121(6):750–754. [PubMed]
  • Bianchi A, Scieux C, Brunat N, Vexiau D, Kermanach M, Pezin P, Janier M, Morel P, Lagrange PH. An evaluation of the polymerase chain reaction amplicor Chlamydia trachomatis in male urine and female urogenital specimens. Sex Transm Dis. 1994 Jul-Aug;21(4):196–200. [PubMed]
  • Biro FM, Reising SF, Doughman JA, Kollar LM, Rosenthal SL. A comparison of diagnostic methods in adolescent girls with and without symptoms of Chlamydia urogenital infection. Pediatrics. 1994 Mar;93(3):476–480. [PubMed]
  • Blanding J, Hirsch L, Stranton N, Wright T, Aarnaes S, de la Maza L, Peterson EM. Comparison of the Clearview Chlamydia, the PACE 2 assay, and culture for detection of Chlamydia trachomatis from cervical specimens in a low-prevalence population. J Clin Microbiol. 1993 Jun;31(6):1622–1625. [PMC free article] [PubMed]
  • Bleker OP, Smalbraak DJ, Schutte MF. Bartholin's abscess: the role of Chlamydia trachomatis. Genitourin Med. 1990 Feb;66(1):24–25. [PMC free article] [PubMed]
  • Blythe MJ, Katz BP, Batteiger BE, Ganser JA, Jones RB. Recurrent genitourinary chlamydial infections in sexually active female adolescents. J Pediatr. 1992 Sep;121(3):487–493. [PubMed]
  • BOVARNICK MR, MILLER JC, SNYDER JC. The influence of certain salts, amino acids, sugars, and proteins on the stability of rickettsiae. J Bacteriol. 1950 Apr;59(4):509–522. [PMC free article] [PubMed]
  • Cates W, Jr, Wasserheit JN. Genital chlamydial infections: epidemiology and reproductive sequelae. Am J Obstet Gynecol. 1991 Jun;164(6 Pt 2):1771–1781. [PubMed]
  • Chan EL, Brandt K, Horsman GB. A 1-year evaluation of Syva MicroTrak Chlamydia enzyme immunoassay with selective confirmation by direct fluorescent-antibody assay in a high-volume laboratory. J Clin Microbiol. 1994 Sep;32(9):2208–2211. [PMC free article] [PubMed]
  • Chan SW. Evaluation of Pharmacia Chlamydia EIA for diagnosis of genital infections caused by Chlamydia trachomatis. Pathology. 1993 Jan;25(1):68–70. [PubMed]
  • Chernesky M, Castriciano S, Sellors J, Stewart I, Cunningham I, Landis S, Seidelman W, Grant L, Devlin C, Mahony J. Detection of Chlamydia trachomatis antigens in urine as an alternative to swabs and cultures. J Infect Dis. 1990 Jan;161(1):124–126. [PubMed]
  • Chernesky MA, Jang D, Lee H, Burczak JD, Hu H, Sellors J, Tomazic-Allen SJ, Mahony JB. Diagnosis of Chlamydia trachomatis infections in men and women by testing first-void urine by ligase chain reaction. J Clin Microbiol. 1994 Nov;32(11):2682–2685. [PMC free article] [PubMed]
  • Chernesky M, Jang D, Sellors J, Castriciano S, Pickard L, Luinstra K, Mahony J. Confirmatory testing demonstrates that false-positive rates in the chlamydiazyme assay are influenced by gender and genital specimen type. Sex Transm Dis. 1993 Nov-Dec;20(6):301–306. [PubMed]
  • Chernesky MA, Jang D, Sellors J, Coleman P, Bodner J, Hrusovsky I, Chong S, Mahony JB. Detection of Chlamydia trachomatis antigens in male urethral swabs and urines with a microparticle enzyme immunoassay. Sex Transm Dis. 1995 Jan-Feb;22(1):55–59. [PubMed]
  • Chernesky MA, Lee H, Schachter J, Burczak JD, Stamm WE, McCormack WM, Quinn TC. Diagnosis of Chlamydia trachomatis urethral infection in symptomatic and asymptomatic men by testing first-void urine in a ligase chain reaction assay. J Infect Dis. 1994 Nov;170(5):1308–1311. [PubMed]
  • Chernesky MA, Mahony JB, Castriciano S, Mores M, Stewart IO, Landis SJ, Seidelman W, Sargeant EJ, Leman C. Detection of Chlamydia trachomatis antigens by enzyme immunoassay and immunofluorescence in genital specimens from symptomatic and asymptomatic men and women. J Infect Dis. 1986 Jul;154(1):141–148. [PubMed]
  • Clad A, Freidank H, Plünnecke J, Jung B, Petersen EE. Chlamydia trachomatis species specific serology: ImmunoComb Chlamydia bivalent versus microimmunofluorescence (MIF). Infection. 1994 May-Jun;22(3):165–173. [PubMed]
  • Claesson BA, Trollfors B, Brolin I, Granström M, Henrichsen J, Jodal U, Juto P, Kallings I, Kanclerski K, Lagergård T, et al. Etiology of community-acquired pneumonia in children based on antibody responses to bacterial and viral antigens. Pediatr Infect Dis J. 1989 Dec;8(12):856–862. [PubMed]
  • Clark A, Stamm WE, Gaydos C, Welsh L, Quinn TC, Schachter J, Moncada J. Multicenter evaluation of the AntigEnz Chlamydia enzyme immunoassay for diagnosis of Chlamydia trachomatis genital infection. J Clin Microbiol. 1992 Nov;30(11):2762–2764. [PMC free article] [PubMed]
  • Clarke LM, Sierra MF, Daidone BJ, Lopez N, Covino JM, McCormack WM. Comparison of the Syva MicroTrak enzyme immunoassay and Gen-Probe PACE 2 with cell culture for diagnosis of cervical Chlamydia trachomatis infection in a high-prevalence female population. J Clin Microbiol. 1993 Apr;31(4):968–971. [PMC free article] [PubMed]
  • Cottingham J, Hunter D. Chlamydia trachomatis and oral contraceptive use: a quantitative review. Genitourin Med. 1992 Aug;68(4):209–216. [PMC free article] [PubMed]
  • Crowley T, Milne D, Arumainayagam JT, Paul ID, Caul EO. The laboratory diagnosis of male Chlamydia trachomatis infections--a time for change? J Infect. 1992 Jul;25 (Suppl 1):69–75. [PubMed]
  • Demaio J, Boyd RS, Rensi R, Clark A. False-positive Chlamydiazyme results during urine sediment analysis due to bacterial urinary tract infections. J Clin Microbiol. 1991 Jul;29(7):1436–1438. [PMC free article] [PubMed]
  • Dille BJ, Butzen CC, Birkenmeyer LG. Amplification of Chlamydia trachomatis DNA by ligase chain reaction. J Clin Microbiol. 1993 Mar;31(3):729–731. [PMC free article] [PubMed]
  • Domeika M, Bassiri M, Mårdh PA. Diagnosis of genital Chlamydia trachomatis infections in asymptomatic males by testing urine by PCR. J Clin Microbiol. 1994 Oct;32(10):2350–2352. [PMC free article] [PubMed]
  • Dunlop EM, Goh BT, Darougar S, Woodland R. Triple-culture tests for diagnosis of chlamydial infection of the female genital tract. Sex Transm Dis. 1985 Apr-Jun;12(2):68–71. [PubMed]
  • Ehret JM, Leszcynski JC, Douglas JM, Genova SL, Chernesky MA, Moncada J, Schachter J. Evaluation of chlamydiazyme enzyme immunoassay for detection of Chlamydia trachomatis in urine specimens from men. J Clin Microbiol. 1993 Oct;31(10):2702–2705. [PMC free article] [PubMed]
  • Embil JA, Thiébaux HJ, Manuel FR, Pereira LH, MacDonald SW. Sequential cervical specimens and the isolation of Chlamydia trachomatis: factors affecting detection. Sex Transm Dis. 1983 Apr-Jun;10(2):62–66. [PubMed]
  • Ferris DG, Martin WH. A comparison of three rapid chlamydial tests in pregnant and nonpregnant women. J Fam Pract. 1992 May;34(5):593–597. [PubMed]
  • Friis B, Kuo CC, Wang SP, Mordhorst CH, Grayston JT. Rapid diagnosis of Chlamydia trachomatis pneumonia in infants. Acta Pathol Microbiol Immunol Scand B. 1984 Jun;92(3):139–143. [PubMed]
  • Gaydos CA, Reichart CA, Long JM, Welsh LE, Neumann TM, Hook EW, 3rd, Quinn TC. Evaluation of Syva enzyme immunoassay for detection of Chlamydia trachomatis in genital specimens. J Clin Microbiol. 1990 Jul;28(7):1541–1544. [PMC free article] [PubMed]
  • Genç M, Domeika M, Mårdh PA. Cost-effectiveness of screening for Chlamydia using DNA amplification. JAMA. 1994 Jun 8;271(22):1741–1741. [PubMed]
  • Genç M, Ruusuvaara L, Mårdh PA. An economic evaluation of screening for Chlamydia trachomatis in adolescent males. JAMA. 1993 Nov 3;270(17):2057–2064. [PubMed]
  • Genç M, Stary A, Bergman S, Mårdh PA. Detection of Chlamydia trachomatis in first-void urine collected from men and women attending a venereal clinic. APMIS. 1991 May;99(5):455–459. [PubMed]
  • Gordon FB, Harper IA, Quan AL, Treharne JD, Dwyer RS, Garland JA. Detection of Chlamydia (Bedsonia) in certain infections of man. I. Laboratory procedures: comparison of yolk sac and cell culture for detection and isolation. J Infect Dis. 1969 Oct;120(4):451–462. [PubMed]
  • Grayston JT. Infections caused by Chlamydia pneumoniae strain TWAR. Clin Infect Dis. 1992 Nov;15(5):757–761. [PubMed]
  • Grossman JH, 3rd, Rivlin ME, Morrison JC. Diagnosis of chlamydial infection in pregnant women using the Testpack Chlamydia diagnostic kit. Obstet Gynecol. 1991 May;77(5):801–803. [PubMed]
  • Hallander H, Jonsson P, Gästrin B. Value of confirmation of Chlamydiazyme enzyme immunoassay results in the detection of Chlamydia trachomatis. Eur J Clin Microbiol Infect Dis. 1992 Jun;11(6):550–552. [PubMed]
  • Hammerschlag MR. Chlamydia trachomatis in children. Pediatr Ann. 1994 Jul;23(7):349–353. [PubMed]
  • Hammerschlag MR, Cummings C, Roblin PM, Williams TH, Delke I. Efficacy of neonatal ocular prophylaxis for the prevention of chlamydial and gonococcal conjunctivitis. N Engl J Med. 1989 Mar 23;320(12):769–772. [PubMed]
  • Hammerschlag MR, Doraiswamy B, Alexander ER, Cox P, Price W, Gleyzer A. Are rectogenital chlamydial infections a marker of sexual abuse in children? Pediatr Infect Dis. 1984 Mar-Apr;3(2):100–104. [PubMed]
  • Han Y, Morse DL, Lawrence CE, Murphy D, Hipp S. Risk profile for Chlamydia infection in women from public health clinics in New York State. J Community Health. 1993 Feb;18(1):1–9. [PubMed]
  • Hardy PH, Hardy JB, Nell EE, Graham DA, Spence MR, Rosenbaum RC. Prevalence of six sexually transmitted disease agents among pregnant inner-city adolescents and pregnancy outcome. Lancet. 1984 Aug 11;2(8398):333–337. [PubMed]
  • Harrison HR, Alexander ER, Weinstein L, Lewis M, Nash M, Sim DA. Cervical Chlamydia trachomatis and mycoplasmal infections in pregnancy. Epidemiology and outcomes. JAMA. 1983 Oct 7;250(13):1721–1727. [PubMed]
  • Harrison HR, Costin M, Meder JB, Bownds LM, Sim DA, Lewis M, Alexander ER. Cervical Chlamydia trachomatis infection in university women: relationship to history, contraception, ectopy, and cervicitis. Am J Obstet Gynecol. 1985 Oct 1;153(3):244–251. [PubMed]
  • Hay PE, Thomas BJ, Horner PJ, MacLeod E, Renton AM, Taylor-Robinson D. Chlamydia trachomatis in women: the more you look, the more you find. Genitourin Med. 1994 Apr;70(2):97–100. [PMC free article] [PubMed]
  • Herbrink P, Zuyderwijk-Zwinkels M, Niesters B, Wagenvoort J, Van Dijk W. Comparison of different culture media for isolation of Chlamydia trachomatis by cell culture on HeLa cells. Eur J Clin Microbiol Infect Dis. 1991 Aug;10(8):656–659. [PubMed]
  • Hillis SD, Nakashima A, Marchbanks PA, Addiss DG, Davis JP. Risk factors for recurrent Chlamydia trachomatis infections in women. Am J Obstet Gynecol. 1994 Mar;170(3):801–806. [PubMed]
  • Hobson D, Karayiannis P, Byng RE, Rees E, Tait IA, Davies JA. Quantitative aspects of chlamydial infection of the cervix. Br J Vener Dis. 1980 Jun;56(3):156–162. [PMC free article] [PubMed]
  • Holmes KK, Handsfield HH, Wang SP, Wentworth BB, Turck M, Anderson JB, Alexander ER. Etiology of nongonococcal urethritis. N Engl J Med. 1975 Jun 5;292(23):1199–1205. [PubMed]
  • Hosein IK, Kaunitz AM, Craft SJ. Detection of cervical Chlamydia trachomatis and Neisseria gonorrhoeae with deoxyribonucleic acid probe assays in obstetric patients. Am J Obstet Gynecol. 1992 Sep;167(3):588–591. [PubMed]
  • Howard C, Friedman DL, Leete JK, Christensen ML. Correlation of the percent of positive Chlamydia trachomatis direct fluorescent antibody detection tests with the adequacy of specimen collection. Diagn Microbiol Infect Dis. 1991 May-Jun;14(3):233–237. [PubMed]
  • Ingram DL, Runyan DK, Collins AD, White ST, Durfee MF, Pearson AW, Occhiuti AR. Vaginal Chlamydia trachomatis infection in children with sexual contact. Pediatr Infect Dis. 1984 Mar-Apr;3(2):97–99. [PubMed]
  • Ingram DL, White ST, Occhiuti AR, Lyna PR. Childhood vaginal infections: association of Chlamydia trachomatis with sexual contact. Pediatr Infect Dis. 1986 Mar-Apr;5(2):226–229. [PubMed]
  • Iwen PC, Blair TM, Woods GL. Comparison of the Gen-Probe PACE 2 system, direct fluorescent-antibody, and cell culture for detecting Chlamydia trachomatis in cervical specimens. Am J Clin Pathol. 1991 Apr;95(4):578–582. [PubMed]
  • Jang D, Sellors JW, Mahony JB, Pickard L, Chernesky MA. Effects of broadening the gold standard on the performance of a chemiluminometric immunoassay to detect Chlamydia trachomatis antigens in centrifuged first void urine and urethral swab samples from men. Sex Transm Dis. 1992 Nov-Dec;19(6):315–319. [PubMed]
  • Jaschek G, Gaydos CA, Welsh LE, Quinn TC. Direct detection of Chlamydia trachomatis in urine specimens from symptomatic and asymptomatic men by using a rapid polymerase chain reaction assay. J Clin Microbiol. 1993 May;31(5):1209–1212. [PMC free article] [PubMed]
  • Johnson J, Neas B, Parker DE, Fortenberry JD, Cowan LD. Screening for urethral infection in adolescent and young adult males. J Adolesc Health. 1993 Jul;14(5):356–361. [PubMed]
  • Johnston SL, Siegel C. Comparison of Buffalo green monkey kidney cells and McCoy cells for the isolation of Chlamydia trachomatis in shell vial centrifugation culture. Diagn Microbiol Infect Dis. 1992 May-Jun;15(4):355–357. [PubMed]
  • Jones RB, Katz BP, van der Pol B, Caine VA, Batteiger BE, Newhall WJ. Effect of blind passage and multiple sampling on recovery of Chlamydia trachomatis from urogenital specimens. J Clin Microbiol. 1986 Dec;24(6):1029–1033. [PMC free article] [PubMed]
  • Jones RB, Rabinovitch RA, Katz BP, Batteiger BE, Quinn TS, Terho P, Lapworth MA. Chlamydia trachomatis in the pharynx and rectum of heterosexual patients at risk for genital infection. Ann Intern Med. 1985 Jun;102(6):757–762. [PubMed]
  • Judson FN, Ehret J. Laboratory diagnosis of sexually transmitted infections. Pediatr Ann. 1994 Jul;23(7):361–369. [PubMed]
  • Karimi ST, Schloemer RH, Wilde CE., 3rd Accumulation of chlamydial lipopolysaccharide antigen in the plasma membranes of infected cells. Infect Immun. 1989 Jun;57(6):1780–1785. [PMC free article] [PubMed]
  • Kellogg JA, Seiple JW, Hick ME. Cross-reaction of clinical isolates of bacteria and yeasts with the chlamydiazyme test for chlamydial antigen, before and after use of a blocking reagent. Am J Clin Pathol. 1992 Mar;97(3):309–312. [PubMed]
  • Kellogg JA, Seiple JW, Klinedinst JL, Levisky JS. Impact of endocervical specimen quality on apparent prevalence of Chlamydia trachomatis infections diagnosed using an enzyme-linked immunosorbent assay method. Arch Pathol Lab Med. 1991 Dec;115(12):1223–1227. [PubMed]
  • Kellogg JA, Seiple JW, Klinedinst JL, Levisky JS. Comparison of cytobrushes with swabs for recovery of endocervical cells and for Chlamydiazyme detection of Chlamydia trachomatis. J Clin Microbiol. 1992 Nov;30(11):2988–2990. [PMC free article] [PubMed]
  • Kellogg JA, Seiple JW, Stroll ES. Direct fluorescent-antibody confirmation of chlamydial antigen below the detection threshold of the chlamydiazyme enzyme-linked immunosorbent assay. J Clin Microbiol. 1993 Jun;31(6):1646–1647. [PMC free article] [PubMed]
  • Kellogg JA, Vanderhoff BT, Seiple JW, Hick ME. Comparison of first-voided urine specimens with endocervical swab specimens for enzyme-linked immunosorbent assay detection of Chlamydia trachomatis in women. Arch Fam Med. 1994 Aug;3(8):672–675. [PubMed]
  • Kluytmans JA, Goessens WH, Mouton JW, van Rijsoort-Vos JH, Niesters HG, Quint WG, Habbema L, Stolz E, Wagenvoort JH. Evaluation of Clearview and Magic Lite tests, polymerase chain reaction, and cell culture for detection of Chlamydia trachomatis in urogenital specimens. J Clin Microbiol. 1993 Dec;31(12):3204–3210. [PMC free article] [PubMed]
  • Kluytmans JA, Goessens WH, van Rijsoort-Vos JH, Niesters HG, Stolz E. Improved performance of PACE 2 with modified collection system in combination with probe competition assay for detection of Chlamydia trachomatis in urethral specimens from males. J Clin Microbiol. 1994 Feb;32(2):568–570. [PMC free article] [PubMed]
  • Kluytmans JA, Niesters HG, Mouton JW, Quint WG, Ijpelaar JA, Van Rijsoort-Vos JH, Habbema L, Stolz E, Michel MF, Wagenvoort JH. Performance of a nonisotopic DNA probe for detection of Chlamydia trachomatis in urogenital specimens. J Clin Microbiol. 1991 Dec;29(12):2685–2689. [PMC free article] [PubMed]
  • Kluytmans JA, van der Willigen AH, van Heyst BY, van der Meyden WI, Stolz E, Wagenvoort JH. Evaluation of an enzyme immunoassay for detection of Chlamydia trachomatis in urogenital specimens. Int J STD AIDS. 1990 Jan;1(1):49–52. [PubMed]
  • Kok TW, Payne LE, Bailey SE, Waddell RG. Urine and the laboratory diagnosis of Chlamydia trachomatis in males. Genitourin Med. 1993 Feb;69(1):51–53. [PMC free article] [PubMed]
  • Krech T, Bleckmann M, Paatz R. Comparison of buffalo green monkey cells and McCoy cells for isolation of Chlamydia trachomatis in a microtiter system. J Clin Microbiol. 1989 Oct;27(10):2364–2365. [PMC free article] [PubMed]
  • Krul K. Closing in on Chlamydia. CAP Today. 1995 May;9(5):1–20. [PubMed]
  • Kuo C, Wang S, Wentworth BB, Grayston JT. Primary isolation of TRIC organisms in HeLa 229 cells treated with DEAE-dextran. J Infect Dis. 1972 Jun;125(6):665–668. [PubMed]
  • Laga M, Manoka A, Kivuvu M, Malele B, Tuliza M, Nzila N, Goeman J, Behets F, Batter V, Alary M, et al. Non-ulcerative sexually transmitted diseases as risk factors for HIV-1 transmission in women: results from a cohort study. AIDS. 1993 Jan;7(1):95–102. [PubMed]
  • Lampe MF, Suchland RJ, Stamm WE. Nucleotide sequence of the variable domains within the major outer membrane protein gene from serovariants of Chlamydia trachomatis. Infect Immun. 1993 Jan;61(1):213–219. [PMC free article] [PubMed]
  • Lee HH, Chernesky MA, Schachter J, Burczak JD, Andrews WW, Muldoon S, Leckie G, Stamm WE. Diagnosis of Chlamydia trachomatis genitourinary infection in women by ligase chain reaction assay of urine. Lancet. 1995 Jan 28;345(8944):213–216. [PubMed]
  • Lees MI, Newnan DM, Garland SM. Comparison of a DNA probe assay with culture for the detection of Chlamydia trachomatis. J Med Microbiol. 1991 Sep;35(3):159–161. [PubMed]
  • Leonardi GP, Seitz M, Edstrom R, Cruz J, Costello P, Szabo K. Evaluation of three immunoassays for detection of Chlamydia trachomatis in urine specimens from asymptomatic males. J Clin Microbiol. 1992 Nov;30(11):2793–2796. [PMC free article] [PubMed]
  • Limberger RJ, Biega R, Evancoe A, McCarthy L, Slivienski L, Kirkwood M. Evaluation of culture and the Gen-Probe PACE 2 assay for detection of Neisseria gonorrhoeae and Chlamydia trachomatis in endocervical specimens transported to a state health laboratory. J Clin Microbiol. 1992 May;30(5):1162–1166. [PMC free article] [PubMed]
  • Lin JS, Jones WE, Yan L, Wirthwein KA, Flaherty EE, Haivanis RM, Rice PA. Underdiagnosis of Chlamydia trachomatis infection. Diagnostic limitations in patients with low-level infection. Sex Transm Dis. 1992 Sep-Oct;19(5):259–265. [PubMed]
  • Lipkin ES, Moncada JV, Shafer MA, Wilson TE, Schachter J. Comparison of monoclonal antibody staining and culture in diagnosing cervical chlamydial infection. J Clin Microbiol. 1986 Jan;23(1):114–117. [PMC free article] [PubMed]
  • Loeffelholz MJ, Lewinski CA, Silver SR, Purohit AP, Herman SA, Buonagurio DA, Dragon EA. Detection of Chlamydia trachomatis in endocervical specimens by polymerase chain reaction. J Clin Microbiol. 1992 Nov;30(11):2847–2851. [PMC free article] [PubMed]
  • Maass M, Dalhoff K. Comparison of sample preparation methods for detection of Chlamydia pneumoniae in bronchoalveolar lavage fluid by PCR. J Clin Microbiol. 1994 Oct;32(10):2616–2619. [PMC free article] [PubMed]
  • Magder LS, Harrison HR, Ehret JM, Anderson TS, Judson FN. Factors related to genital Chlamydia trachomatis and its diagnosis by culture in a sexually transmitted disease clinic. Am J Epidemiol. 1988 Aug;128(2):298–308. [PubMed]
  • Magder LS, Klontz KC, Bush LH, Barnes RC. Effect of patient characteristics on performance of an enzyme immunoassay for detecting cervical Chlamydia trachomatis infection. J Clin Microbiol. 1990 Apr;28(4):781–784. [PMC free article] [PubMed]
  • Mahony JB, Chernesky MA. Effect of swab type and storage temperature on the isolation of Chlamydia trachomatis from clinical specimens. J Clin Microbiol. 1985 Nov;22(5):865–867. [PMC free article] [PubMed]
  • Tyndall MW, Nasio J, Maitha G, Ndinya-Achola JO, Plummer FA, Sellors JW, Luinstra KE, Jang D, Mahony JB, Chernesky MA. Leukocyte esterase urine strips for the screening of men with urethritis--use in developing countries. Genitourin Med. 1994 Feb;70(1):3–6. [PMC free article] [PubMed]
  • Mahony JB, Chernesky MA, Bromberg K, Schachter J. Accuracy of immunoglobulin M immunoassay for diagnosis of chlamydial infections in infants and adults. J Clin Microbiol. 1986 Nov;24(5):731–735. [PMC free article] [PubMed]
  • Mahony JB, Luinstra KE, Sellors JW, Chernesky MA. Comparison of plasmid- and chromosome-based polymerase chain reaction assays for detecting Chlamydia trachomatis nucleic acids. J Clin Microbiol. 1993 Jul;31(7):1753–1758. [PMC free article] [PubMed]
  • Mahony JB, Luinstra KE, Sellors JW, Pickard L, Chong S, Jang D, Chernesky MA. Role of confirmatory PCRs in determining performance of Chlamydia Amplicor PCR with endocervical specimens from women with a low prevalence of infection. J Clin Microbiol. 1994 Oct;32(10):2490–2493. [PMC free article] [PubMed]
  • Mårdh PA, Zeeberg B. Toxic effect of sampling swabs and transportation test tubes on the formation of intracytoplasmic inclusions of Chlamydia trachomatis in McCoy cell cultures. Br J Vener Dis. 1981 Aug;57(4):268–272. [PMC free article] [PubMed]
  • Martin DH. Chlamydial infections. Med Clin North Am. 1990 Nov;74(6):1367–1387. [PubMed]
  • Martin DH, Koutsky L, Eschenbach DA, Daling JR, Alexander ER, Benedetti JK, Holmes KK. Prematurity and perinatal mortality in pregnancies complicated by maternal Chlamydia trachomatis infections. JAMA. 1982 Mar 19;247(11):1585–1588. [PubMed]
  • Matthews RS, Pandit PG, Bonigal SD, Wise R, Radcliffe KW. Evaluation of an enzyme-linked immunoassay and confirmatory test for the detection of Chlamydia trachomatis in male urine samples. Genitourin Med. 1993 Feb;69(1):47–50. [PMC free article] [PubMed]
  • McComb DE, Puzniak CI. Micro cell culture method for isolation of Chlamydia trachomatis. Appl Microbiol. 1974 Oct;28(4):727–729. [PMC free article] [PubMed]
  • McNagny SE, Parker RM, Zenilman JM, Lewis JS. Urinary leukocyte esterase test: a screening method for the detection of asymptomatic chlamydial and gonococcal infections in men. J Infect Dis. 1992 Mar;165(3):573–576. [PubMed]
  • EDDIE B, MEYER KF. The influence of tetracycline compounds on the development of antibodies in psittacosis. Am Rev Tuberc. 1956 Oct;74(4):566–571. [PubMed]
  • Mills RD, Young A, Cain K, Blair TM, Sitorius MA, Woods GL. Chlamydiazyme plus blocking assay to detect Chlamydia trachomatis in endocervical specimens. Am J Clin Pathol. 1992 Feb;97(2):209–212. [PubMed]
  • Moncada J, Schachter J, Bolan G, Engelman J, Howard L, Mushahwar I, Ridgway G, Mumtaz G, Stamm W, Clark A. Confirmatory assay increases specificity of the chlamydiazyme test for Chlamydia trachomatis infection of the cervix. J Clin Microbiol. 1990 Aug;28(8):1770–1773. [PMC free article] [PubMed]
  • Moncada J, Schachter J, Bolan G, Nathan J, Shafer MA, Clark A, Schwebke J, Stamm W, Mroczkowski T, Seliborska Z, et al. Evaluation of Syva's enzyme immunoassay for the detection of Chlamydia trachomatis in urogenital specimens. Diagn Microbiol Infect Dis. 1992 Nov-Dec;15(8):663–668. [PubMed]
  • Moncada J, Schachter J, Shafer MA, Williams E, Gourlay L, Lavin B, Bolan G. Detection of Chlamydia trachomatis in first catch urine samples from symptomatic and asymptomatic males. Sex Transm Dis. 1994 Jan-Feb;21(1):8–12. [PubMed]
  • Moncada J, Schachter J, Shipp M, Bolan G, Wilber J. Cytobrush in collection of cervical specimens for detection of Chlamydia trachomatis. J Clin Microbiol. 1989 Aug;27(8):1863–1866. [PMC free article] [PubMed]
  • Morris RE, Legault J, Baker C. Prevalence of isolated urethral asymptomatic Chlamydia trachomatis infection in the absence of cervical infection in incarcerated adolescent girls. Sex Transm Dis. 1993 Jul-Aug;20(4):198–200. [PubMed]
  • Moss TR, Darougar S, Woodland RM, Nathan M, Dines RJ, Cathrine V. Antibodies to Chlamydia species in patients attending a genitourinary clinic and the impact of antibodies to C. pneumoniae and C. psittaci on the sensitivity and the specificity of C. trachomatis serology tests. Sex Transm Dis. 1993 Mar-Apr;20(2):61–65. [PubMed]
  • Mullis KB, Faloona FA. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods Enzymol. 1987;155:335–350. [PubMed]
  • Mumtaz G, Ridgway GL, Clark S, Allason-Jones E. Evaluation of an enzyme immunoassay (Chlamydiazyme) with confirmatory test for the detection of chlamydial antigen in urine from men. Int J STD AIDS. 1991 Sep-Oct;2(5):359–361. [PubMed]
  • Olsen MA, Sambol AR, Bohnert VA. Comparison of the Syva Microtrak enzyme immunoassay and Abbott Chlamydiazyme in the detection of chlamydial infections in women. Arch Pathol Lab Med. 1995 Feb;119(2):153–156. [PubMed]
  • Ossewaarde JM, Rieffe M. Storage conditions of Chlamydia trachomatis antigens. Eur J Clin Microbiol Infect Dis. 1989 Jul;8(7):658–660. [PubMed]
  • Ossewaarde JM, Rieffe M, Rozenberg-Arska M, Ossenkoppele PM, Nawrocki RP, van Loon AM. Development and clinical evaluation of a polymerase chain reaction test for detection of Chlamydia trachomatis. J Clin Microbiol. 1992 Aug;30(8):2122–2128. [PMC free article] [PubMed]
  • Paavonen J, Vesterinen E. Chlamydia trachomatis in cervicitis and urethritis in women. Scand J Infect Dis Suppl. 1982;32:45–54. [PubMed]
  • Paisley JW, Lauer BA, Melinkovich P, Gitterman BA, Feiten DJ, Berman S. Rapid diagnosis of Chlamydia trachomatis pneumonia in infants by direct immunofluorescence microscopy of nasopharyngeal secretions. J Pediatr. 1986 Oct;109(4):653–655. [PubMed]
  • Patrick DM, Rekart ML, Knowles L. Unsatisfactory performance of the leukocyte esterase test of first voided urine for rapid diagnosis of urethritis. Genitourin Med. 1994 Jun;70(3):187–190. [PMC free article] [PubMed]
  • Pearlman MD, McNeeley SG. A review of the microbiology, immunology, and clinical implications of Chlamydia trachomatis infections. Obstet Gynecol Surv. 1992 Jul;47(7):448–461. [PubMed]
  • Peeling RW, Brunham RC. Molecular techniques for the laboratory identification of Chlamydia trachomatis. J Int Fed Clin Chem. 1994 Jun;6(3):78–82. [PubMed]
  • Peterson EM, Markoff BA, Schachter J, de la Maza LM. The 7.5-kb plasmid present in Chlamydia trachomatis is not essential for the growth of this microorganism. Plasmid. 1990 Mar;23(2):144–148. [PubMed]
  • Phillips RS, Aronson MD, Taylor WC, Safran C. Should tests for Chlamydia trachomatis cervical infection be done during routine gynecologic visits? An analysis of the costs of alternative strategies. Ann Intern Med. 1987 Aug;107(2):188–194. [PubMed]
  • Phillips RS, Hanff PA, Kauffman RS, Aronson MD. Use of a direct fluorescent antibody test for detecting Chlamydia trachomatis cervical infection in women seeking routine gynecologic care. J Infect Dis. 1987 Oct;156(4):575–581. [PubMed]
  • Pothier P, Kazmierczak A. Comparison of cell culture with two direct Chlamydia tests using immunofluorescence or enzyme-linked immunosorbent assay. Eur J Clin Microbiol. 1986 Oct;5(5):569–572. [PubMed]
  • Preece PM, Anderson JM, Thompson RG. Chlamydia trachomatis infection in infants: a prospective study. Arch Dis Child. 1989 Apr;64(4):525–529. [PMC free article] [PubMed]
  • Quinn TC, Gupta PK, Burkman RT, Kappus EW, Barbacci M, Spence MR. Detection of Chlamydia trachomatis cervical infection: a comparison of Papanicolaou and immunofluorescent staining with cell culture. Am J Obstet Gynecol. 1987 Aug;157(2):394–399. [PubMed]
  • Rapoza PA, Quinn TC, Kiessling LA, Green WR, Taylor HR. Assessment of neonatal conjunctivitis with a direct immunofluorescent monoclonal antibody stain for Chlamydia. JAMA. 1986 Jun 27;255(24):3369–3373. [PubMed]
  • Reeve P, Owen J, Oriel JD. Laboratory procedures for the isolation of chlamydia trachomatis from the human genital tract. J Clin Pathol. 1975 Nov;28(11):910–914. [PMC free article] [PubMed]
  • Rietmeijer CA, Judson FN, Van Hensbroek MB, Ehret JM, Douglas JM., Jr Unsuspected Chlamydia trachomatis infection in heterosexual men attending a sexually transmitted diseases clinic: evaluation of risk factors and screening methods. Sex Transm Dis. 1991 Jan-Mar;18(1):28–35. [PubMed]
  • Rompalo AM, Roberts P, Johnson K, Stamm WE. Empirical therapy for the management of acute proctitis in homosexual men. JAMA. 1988 Jul 15;260(3):348–353. [PubMed]
  • Rompalo AM, Suchland RJ, Price CB, Stamm WE. Rapid diagnosis of Chlamydia trachomatis rectal infection by direct immunofluorescence staining. J Infect Dis. 1987 May;155(5):1075–1076. [PubMed]
  • Roosendaal R, Walboomers JM, Veltman OR, Melgers I, Burger C, Bleker OP, MacClaren DM, Meijer CJ, van den Brule AJ. Comparison of different primer sets for detection of Chlamydia trachomatis by the polymerase chain reaction. J Med Microbiol. 1993 Jun;38(6):426–433. [PubMed]
  • Rota TR, Nichols RL. Infection of cell cultures by trachoma agent: enhancement by DEAE-dextran. J Infect Dis. 1971 Oct;124(4):419–421. [PubMed]
  • Rota TR, Nichols RL. Chlamydin trachomatis in cell culture. I. Comparison of efficiencies of infection in several chemically defined media, at various pH and temperature values, and after exposure to diethylaminoethyl-dextran. Appl Microbiol. 1973 Oct;26(4):560–565. [PMC free article] [PubMed]
  • Ryan GM, Jr, Abdella TN, McNeeley SG, Baselski VS, Drummond DE. Chlamydia trachomatis infection in pregnancy and effect of treatment on outcome. Am J Obstet Gynecol. 1990 Jan;162(1):34–39. [PubMed]
  • Saiki RK, Scharf S, Faloona F, Mullis KB, Horn GT, Erlich HA, Arnheim N. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science. 1985 Dec 20;230(4732):1350–1354. [PubMed]
  • Sanders JW, Hook EW, 3rd, Welsh LE, Shepherd ME, Quinn TC. Evaluation of an enzyme immunoassay for detection of Chlamydia trachomatis in urine of asymptomatic men. J Clin Microbiol. 1994 Jan;32(1):24–27. [PMC free article] [PubMed]
  • Schachter J. Why we need a program for the control of Chlamydia trachomatis. N Engl J Med. 1989 Mar 23;320(12):802–804. [PubMed]
  • Schachter J, Grossman M, Sweet RL, Holt J, Jordan C, Bishop E. Prospective study of perinatal transmission of Chlamydia trachomatis. JAMA. 1986 Jun 27;255(24):3374–3377. [PubMed]
  • Schachter J, Stamm WE, Chernesky MA, Hook EW, 3rd, Jones RB, Judson FN, Kellogg JA, LeBar B, Mårdh PA, McCormack WM, et al. Nonculture tests for genital tract chlamydial infection. What does the package insert mean, and will it mean the same thing tomorrow? Sex Transm Dis. 1992 Sep-Oct;19(5):243–244. [PubMed]
  • Schachter J, Stamm WE, Quinn TC, Andrews WW, Burczak JD, Lee HH. Ligase chain reaction to detect Chlamydia trachomatis infection of the cervix. J Clin Microbiol. 1994 Oct;32(10):2540–2543. [PMC free article] [PubMed]
  • Schachter J, Stoner E, Moncada J. Screening for chlamydial infections in women attending family planning clinics. West J Med. 1983 Mar;138(3):375–379. [PMC free article] [PubMed]
  • Schoenwald E, Schmidt BL, Steinmetz G, Hosmann J, Pohla-Gubo G, Luger A, Gasser G. Diagnosis of Chlamydia trachomatis infection--culture versus serology. Eur J Epidemiol. 1988 Mar;4(1):75–82. [PubMed]
  • Schubiner HH, LeBar WD, Joseph S, Taylor C, Jemal C. Evaluation of two rapid tests for the diagnosis of Chlamydia trachomatis genital infections. Eur J Clin Microbiol Infect Dis. 1992 Jun;11(6):553–556. [PubMed]
  • Schwebke JR, Stamm WE, Handsfield HH. Use of sequential enzyme immunoassay and direct fluorescent antibody tests for detection of Chlamydia trachomatis infections in women. J Clin Microbiol. 1990 Nov;28(11):2473–2476. [PMC free article] [PubMed]
  • Sellors J, Chernesky M, Pickard L, Jang D, Walter S, Krepel J, Mahony J. Effect of time elapsed since previous voiding on the detection of Chlamydia trachomatis antigens in urine. Eur J Clin Microbiol Infect Dis. 1993 Apr;12(4):285–289. [PubMed]
  • Sellors JW, Mahony JB, Jang D, Pickard L, Goldsmith CH, Gafni A, Chernesky MA. Comparison of cervical, urethral, and urine specimens for the detection of Chlamydia trachomatis in women. J Infect Dis. 1991 Jul;164(1):205–208. [PubMed]
  • Sellors JW, Mahony JB, Pickard L, Jang D, Groves D, Luinstra KE, Chernesky MA. Screening urine with a leukocyte esterase strip and subsequent chlamydial testing of asymptomatic men attending primary care practitioners. Sex Transm Dis. 1993 May-Jun;20(3):152–157. [PubMed]
  • Sellors JW, Pickard L, Gafni A, Goldsmith CH, Jang D, Mahony JB, Chernesky MA. Effectiveness and efficiency of selective vs universal screening for chlamydial infection in sexually active young women. Arch Intern Med. 1992 Sep;152(9):1837–1844. [PubMed]
  • Shah JS, Liu J, Smith J, Popoff S, Radcliffe G, O'Brien WJ, Serpe G, Olive DM, King W. Novel, ultrasensitive, Q-beta replicase-amplified hybridization assay for detection of Chlamydia trachomatis. J Clin Microbiol. 1994 Nov;32(11):2718–2724. [PMC free article] [PubMed]
  • Skulnick M, Chua R, Simor AE, Low DE, Khosid HE, Fraser S, Lyons E, Legere EA, Kitching DA. Use of the polymerase chain reaction for the detection of Chlamydia trachomatis from endocervical and urine specimens in an asymptomatic low-prevalence population of women. Diagn Microbiol Infect Dis. 1994 Dec;20(4):195–201. [PubMed]
  • Smith JW, Rogers RE, Katz BP, Brickler JF, Lineback PL, Van der Pol B, Jones RB. Diagnosis of chlamydial infection in women attending antenatal and gynecologic clinics. J Clin Microbiol. 1987 May;25(5):868–872. [PMC free article] [PubMed]
  • Stamm WE. Diagnosis of Chlamydia trachomatis genitourinary infections. Ann Intern Med. 1988 May;108(5):710–717. [PubMed]
  • Stamm WE, Cole B. Asymptomatic Chlamydia trachomatis urethritis in men. Sex Transm Dis. 1986 Jul-Sep;13(3):163–165. [PubMed]
  • Stamm WE, Harrison HR, Alexander ER, Cles LD, Spence MR, Quinn TC. Diagnosis of Chlamydia trachomatis infections by direct immunofluorescence staining of genital secretions. A multicenter trial. Ann Intern Med. 1984 Nov;101(5):638–641. [PubMed]
  • Stamm WE, Koutsky LA, Benedetti JK, Jourden JL, Brunham RC, Holmes KK. Chlamydia trachomatis urethral infections in men. Prevalence, risk factors, and clinical manifestations. Ann Intern Med. 1984 Jan;100(1):47–51. [PubMed]
  • Stamm WE, Tam M, Koester M, Cles L. Detection of Chlamydia trachomatis inclusions in Mccoy cell cultures with fluorescein-conjugated monoclonal antibodies. J Clin Microbiol. 1983 Apr;17(4):666–668. [PMC free article] [PubMed]
  • Stary A, Genç M, Heller-Vitouch C, Mårdh PA. Chlamydial antigen detection in urine samples by immunofluorescence tests. Infection. 1992 Mar-Apr;20(2):101–104. [PubMed]
  • Stary A, Teodorowicz L, Hörting-Müller I, Nerad S, Storch M. Evaluation of the Gen-Probe PACE 2 and the Microtrak enzyme immunoassay for diagnosis of Chlamydia trachomatis in urogenital samples. Sex Transm Dis. 1994 Jan-Feb;21(1):26–30. [PubMed]
  • Steingrímsson O, Olafsson JH, Sigvaldadóttir E, Pálsdóttir R. Clinical evaluation of an automated enzyme-linked fluorescent assay for the detection of chlamydial antigen in specimens from high-risk patients. Diagn Microbiol Infect Dis. 1994 Feb;18(2):101–104. [PubMed]
  • Stephens RS, Kuo CC, Tam MR. Sensitivity of immunofluorescence with monoclonal antibodies for detection of Chlamydia trachomatis inclusions in cell culture. J Clin Microbiol. 1982 Jul;16(1):4–7. [PMC free article] [PubMed]
  • Suchland RJ, Stamm WE. Simplified microtiter cell culture method for rapid immunotyping of Chlamydia trachomatis. J Clin Microbiol. 1991 Jul;29(7):1333–1338. [PMC free article] [PubMed]
  • Svensson L, Weström L, Ripa KT, Mårdh PA. Differences in some clinical and laboratory parameters in acute salpingitis related to culture and serologic findings. Am J Obstet Gynecol. 1980 Dec 1;138(7 Pt 2):1017–1021. [PubMed]
  • Sweet RL, Landers DV, Walker C, Schachter J. Chlamydia trachomatis infection and pregnancy outcome. Am J Obstet Gynecol. 1987 Apr;156(4):824–833. [PubMed]
  • Talbot H, Romanowski B. Factors affecting urine EIA sensitivity in the detection of Chlamydia trachomatis in men. Genitourin Med. 1994 Apr;70(2):101–104. [PMC free article] [PubMed]
  • Tam MR, Stamm WE, Handsfield HH, Stephens R, Kuo CC, Holmes KK, Ditzenberger K, Krieger M, Nowinski RC. Culture-independent diagnosis of Chlamydia trachomatis using monoclonal antibodies. N Engl J Med. 1984 May 3;310(18):1146–1150. [PubMed]
  • Thomas B, Gilchrist C, Taylor-Robinson D. Detection of Chlamydia trachomatis by direct immunofluorescence improved by centrifugation of specimens. Eur J Clin Microbiol Infect Dis. 1991 Aug;10(8):659–662. [PubMed]
  • Thomas BJ, MacLeod EJ, Hay PE, Horner PJ, Taylor-Robinson D. Limited value of two widely used enzyme immunoassays for detection of Chlamydia trachomatis in women. Eur J Clin Microbiol Infect Dis. 1994 Aug;13(8):651–655. [PubMed]
  • Thomas BJ, MacLeod EJ, Taylor-Robinson D. Evaluation of sensitivity of 10 diagnostic assays for Chlamydia trachomatis by use of a simple laboratory procedure. J Clin Pathol. 1993 Oct;46(10):912–914. [PMC free article] [PubMed]
  • Trachtenberg AI, Washington AE, Halldorson S. A cost-based decision analysis for Chlamydia screening in California family planning clinics. Obstet Gynecol. 1988 Jan;71(1):101–108. [PubMed]
  • Vandenvelde C, Scheen R, Defoor M, Duys M, Dumon J, Van Beers D. Suppression of the inhibitory effect of denatured albumin on the polymerase chain reaction by sodium octanoate: application to routine clinical detection of hepatitis B virus at its infectivity threshold in serum. J Virol Methods. 1993 May;42(2-3):251–263. [PubMed]
  • Van Dyck E, Samb N, Sarr AD, Van de Velden L, Moran J, Mboup S, Ndoye I, Lamboray JL, Meheus A, Piot P. Accuracy of two enzyme immunoassays and cell culture in the detection of Chlamydia trachomatis in low and high risk populations in Senegal. Eur J Clin Microbiol Infect Dis. 1992 Jun;11(6):527–534. [PubMed]
  • van Gemen B, Kievits T, Schukkink R, van Strijp D, Malek LT, Sooknanan R, Huisman HG, Lens P. Quantification of HIV-1 RNA in plasma using NASBA during HIV-1 primary infection. J Virol Methods. 1993 Jul;43(2):177–187. [PubMed]
  • van Gemen B, van Beuningen R, Nabbe A, van Strijp D, Jurriaans S, Lens P, Kievits T. A one-tube quantitative HIV-1 RNA NASBA nucleic acid amplification assay using electrochemiluminescent (ECL) labelled probes. J Virol Methods. 1994 Sep;49(2):157–167. [PubMed]
  • Walker GT, Fraiser MS, Schram JL, Little MC, Nadeau JG, Malinowski DP. Strand displacement amplification--an isothermal, in vitro DNA amplification technique. Nucleic Acids Res. 1992 Apr 11;20(7):1691–1696. [PMC free article] [PubMed]
  • Wang SP, Grayston JT. Immunologic relationship between genital TRIC, lymphogranuloma venereum, and related organisms in a new microtiter indirect immunofluorescence test. Am J Ophthalmol. 1970 Sep;70(3):367–374. [PubMed]
  • Wang SP, Grayston JT, Alexander ER, Holmes KK. Simplified microimmunofluorescence test with trachoma-lymphogranuloma venereum (Chlamydia trachomatis) antigens for use as a screening test for antibody. J Clin Microbiol. 1975 Mar;1(3):250–255. [PMC free article] [PubMed]
  • Warford AL, Carter TL, Levy RA, Rekrut KA. Comparison of sonicated and nonsonicated specimens for the isolation of Chlamydia trachomatis. Am J Clin Pathol. 1985 May;83(5):625–629. [PubMed]
  • Warren R, Dwyer B, Plackett M, Pettit K, Rizvi N, Baker AM. Comparative evaluation of detection assays for Chlamydia trachomatis. J Clin Microbiol. 1993 Jun;31(6):1663–1666. [PMC free article] [PubMed]
  • Washington AE, Johnson RE, Sanders LL., Jr Chlamydia trachomatis infections in the United States. What are they costing us? JAMA. 1987 Apr 17;257(15):2070–2072. [PubMed]
  • Weinstock H, Dean D, Bolan G. Chlamydia trachomatis infections. Infect Dis Clin North Am. 1994 Dec;8(4):797–819. [PubMed]
  • Weiss SG, Newcomb RW, Beem MO. Pulmonary assessment of children after chlamydial pneumonia of infancy. J Pediatr. 1986 May;108(5 Pt 1):659–664. [PubMed]
  • Werner MJ, Biro FM. Urinary leukocyte esterase screening for asymptomatic sexually transmitted disease in adolescent males. J Adolesc Health. 1991 Jun;12(4):326–328. [PubMed]
  • Weström L, Bengtsson LP, Mårdh PA. Incidence, trends, and risks of ectopic pregnancy in a population of women. Br Med J (Clin Res Ed) 1981 Jan 3;282(6257):15–18. [PMC free article] [PubMed]
  • Wiesenfeld HC, Uhrin M, Dixon BW, Sweet RL. Diagnosis of male Chlamydia trachomatis urethritis by polymerase chain reaction. Sex Transm Dis. 1994 Sep-Oct;21(5):268–271. [PubMed]
  • Wong KH, Skelton SK, Chan YK. Efficient culture of Chlamydia pneumoniae with cell lines derived from the human respiratory tract. J Clin Microbiol. 1992 Jul;30(7):1625–1630. [PMC free article] [PubMed]
  • Woods GL, Bryan JA. Detection of Chlamydia trachomatis by direct fluorescent antibody staining. Results of the College of American Pathologists Proficiency Testing Program, 1986-1992. Arch Pathol Lab Med. 1994 May;118(5):483–488. [PubMed]
  • Workowski KA, Lampe MF, Wong KG, Watts MB, Stamm WE. Long-term eradication of Chlamydia trachomatis genital infection after antimicrobial therapy. Evidence against persistent infection. JAMA. 1993 Nov 3;270(17):2071–2075. [PubMed]
  • Wu CH, Lee MF, Yin SC, Yang DM, Cheng SF. Comparison of polymerase chain reaction, monoclonal antibody based enzyme immunoassay, and cell culture for detection of Chlamydia trachomatis in genital specimens. Sex Transm Dis. 1992 Jul-Aug;19(4):193–197. [PubMed]
  • Yang B, Yolken R, Viscidi R. Quantitative polymerase chain reaction by monitoring enzymatic activity of DNA polymerase. Anal Biochem. 1993 Jan;208(1):110–116. [PubMed]
  • Yang LI, Panke ES, Leist PA, Fry RJ, Lee RF. Detection of Chlamydia trachomatis endocervical infection in asymptomatic and symptomatic women: comparison of deoxyribonucleic acid probe test with tissue culture. Am J Obstet Gynecol. 1991 Nov;165(5 Pt 1):1444–1453. [PubMed]
  • Yoder BL, Stamm WE, Koester CM, Alexander ER. Microtest procedure for isolation of Chlamydia trachomatis. J Clin Microbiol. 1981 Jun;13(6):1036–1039. [PMC free article] [PubMed]
  • Yong EC, Chinn JS, Caldwell HD, Kuo CC. Reticulate bodies as single antigen in Chlamydia trachomatis serology with microimmunofluorescence. J Clin Microbiol. 1979 Sep;10(3):351–356. [PMC free article] [PubMed]
  • Zeeberg B, Thelin I, Schalén C. Chlamydia trachomatis antigen detection by Chlamydiazyme combined with Chlamydia Blocking Reagent verification. Int J STD AIDS. 1992 Sep-Oct;3(5):355–359. [PubMed]
  • Zelin JM, Robinson AJ, Ridgway GL, Allason-Jones E, Williams P. Chlamydial urethritis in heterosexual men attending a genitourinary medicine clinic: prevalence, symptoms, condom usage and partner change. Int J STD AIDS. 1995 Jan-Feb;6(1):27–30. [PubMed]

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