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Antimicrob Agents Chemother. Sep 1989; 33(9): 1588–1591.
PMCID: PMC172707

In vitro susceptibility studies of vancomycin-resistant Enterococcus faecalis.

Abstract

Vancomycin resistance exhibited by Enterococcus faecalis isolates V583, V586, and V587 is described. The vancomycin MICs ranged from 32 to 64 micrograms/ml. Although resistant to vancomycin, the isolates were susceptible to teicoplanin (MIC, less than or equal to 0.5 micrograms/ml). Such a glycopeptide susceptibility profile has not been previously described for E. faecalis. Time kill studies showed that vancomycin resistance adversely affected the synergistic activity that vancomycin and aminoglycoside combinations usually demonstrate against enterococci. However, the ability to detect vancomycin resistance varied with the susceptibility testing method used. Whereas broth microdilution, broth macrodilution, and agar dilution methods detected resistance, disk-agar diffusion and the AutoMicrobic system Gram-Positive GPS-A susceptibility card (Vitek Systems Inc., Hazelwood, Mo.) did not. To detect vancomycin resistance reliably and establish the incidence of such E. faecalis isolates, adjustments in some susceptibility testing methods may be necessary.

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Selected References

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  • Barry AL, Thornsberry C, Jones RN. Evaluation of teicoplanin and vancomycin disk susceptibility tests. J Clin Microbiol. 1986 Jan;23(1):100–103. [PMC free article] [PubMed]
  • Eliopoulos GM, Thauvin C, Gerson B, Moellering RC., Jr In vitro activity and mechanism of action of A21978C1, a novel cyclic lipopeptide antibiotic. Antimicrob Agents Chemother. 1985 Mar;27(3):357–362. [PMC free article] [PubMed]
  • Facklam RR, Collins MD. Identification of Enterococcus species isolated from human infections by a conventional test scheme. J Clin Microbiol. 1989 Apr;27(4):731–734. [PMC free article] [PubMed]
  • Facklam R, Hollis D, Collins MD. Identification of gram-positive coccal and coccobacillary vancomycin-resistant bacteria. J Clin Microbiol. 1989 Apr;27(4):724–730. [PMC free article] [PubMed]
  • Faruki H, Niles AC, Heeren RL, Murray PR. Effect of calcium on in vitro activity of LY146032 against Clostridium difficile. Antimicrob Agents Chemother. 1987 Mar;31(3):461–462. [PMC free article] [PubMed]
  • Fass RJ, Helsel VL. In vitro activity of LY146032 against staphylococci, streptococci, and enterococci. Antimicrob Agents Chemother. 1986 Nov;30(5):781–784. [PMC free article] [PubMed]
  • Harwick HJ, Kalmanson GM, Guze LB. In vitro activity of ampicillin or vancomycin combined with gentamicin or streptomycin against enterococci. Antimicrob Agents Chemother. 1973 Oct;4(4):383–387. [PMC free article] [PubMed]
  • Kaplan AH, Gilligan PH, Facklam RR. Recovery of resistant enterococci during vancomycin prophylaxis. J Clin Microbiol. 1988 Jun;26(6):1216–1218. [PMC free article] [PubMed]
  • Leclercq R, Derlot E, Duval J, Courvalin P. Plasmid-mediated resistance to vancomycin and teicoplanin in Enterococcus faecium. N Engl J Med. 1988 Jul 21;319(3):157–161. [PubMed]
  • Leclercq R, Derlot E, Weber M, Duval J, Courvalin P. Transferable vancomycin and teicoplanin resistance in Enterococcus faecium. Antimicrob Agents Chemother. 1989 Jan;33(1):10–15. [PMC free article] [PubMed]
  • Moellering RC, Jr, Wennersten C, Weinberg AN. Synergy of penicillin and gentamicin against Enterococci. J Infect Dis. 1971 Dec;124 (Suppl):S207–S209. [PubMed]
  • Parenti F, Beretta G, Berti M, Arioli V. Teichomycins, new antibiotics from Actinoplanes teichomyceticus Nov. Sp. I. Description of the producer strain, fermentation studies and biological properties. J Antibiot (Tokyo) 1978 Apr;31(4):276–283. [PubMed]
  • Sahm DF, Torres C. Effects of medium and inoculum variations on screening for high-level aminoglycoside resistance in Enterococcus faecalis. J Clin Microbiol. 1988 Feb;26(2):250–256. [PMC free article] [PubMed]
  • Sahm DF, Torres C. High-content aminoglycoside disks for determining aminoglycoside-penicillin synergy against Enterococcus faecalis. J Clin Microbiol. 1988 Feb;26(2):257–260. [PMC free article] [PubMed]
  • Shlaes DM, Bouvet A, Devine C, Shlaes JH, al-Obeid S, Williamson R. Inducible, transferable resistance to vancomycin in Enterococcus faecalis A256. Antimicrob Agents Chemother. 1989 Feb;33(2):198–203. [PMC free article] [PubMed]
  • Stratton CW, Liu C, Ratner HB, Weeks LS. Bactericidal activity of deptomycin (LY146032) compared with those of ciprofloxacin, vancomycin, and ampicillin against enterococci as determined by kill-kinetic studies. Antimicrob Agents Chemother. 1987 Jul;31(7):1014–1016. [PMC free article] [PubMed]
  • Toala P, McDonald A, Wilcox C, Finland M. Susceptibility of group D streptococcus (enterococcus) to 21 antibiotics in vitro, with special reference to species differences. Am J Med Sci. 1969 Dec;258(6):416–430. [PubMed]
  • Uttley AH, Collins CH, Naidoo J, George RC. Vancomycin-resistant enterococci. Lancet. 1988 Jan 2;1(8575-6):57–58. [PubMed]
  • Wanger AR, Murray BE. Activity of LY146032 against Enterococci with and without high-level aminoglycoside resistance, including two penicillinase-producing strains. Antimicrob Agents Chemother. 1987 Nov;31(11):1779–1781. [PMC free article] [PubMed]
  • Watanakunakorn C, Bakie C. Synergism of vancomycin-gentamicin and vancomycin-streptomycin against enterococci. Antimicrob Agents Chemother. 1973 Aug;4(2):120–124. [PMC free article] [PubMed]
  • Westenfelder GO, Paterson PY, Reisberg BE, Carlson GM. Vancomycin-streptomycin synergism in enterococcal endocarditis. JAMA. 1973 Jan 1;223(1):37–40. [PubMed]
  • Zervos MJ, Kauffman CA, Therasse PM, Bergman AG, Mikesell TS, Schaberg DR. Nosocomial infection by gentamicin-resistant Streptococcus faecalis. An epidemiologic study. Ann Intern Med. 1987 May;106(5):687–691. [PubMed]

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