|
|
Clin Exp Immunol. 1971 September; 9(3): 289–304. | PMCID: PMC1713067 |
Lymphopenic immunologic deficiency in identical twins: Lymphocyte allografting and graft-versus-host disease following treatment with albumin-gradient-separated paternal bone marrow cells Rebecca H. Buckley, W. B. Kremer, D. T. Rowlands, Carolyn C. Huntley, D. B. Amos, and A. T. Huang Abstract The clinical, immunologic and pathologic features of the first recorded examples of lymphopenic immunologic deficiency in twins are presented. Eleven-month-old male identical twin infants were found to be severely lymphopenic and lacked demonstrable cell-mediated immunity and antibody formation. Each first-degree relative differed from the probands by one chromosome at the major human histocompatibility locus, HL-A. Because of their rapidly deteriorating clinical conditions, allogeneic bone marrow transplantation was undertaken. Circumvention of graft-versus-host disease was attempted by separation of donor bone marrow cells on a discontinuous-albumin-gradient and administration of only 5×106 immature nucleated marrow cells per kilogram infant body weight. Additionally, immunologic enhancement was attempted by pretreating the infants with human isoantisera to identifiable infant HL-A antigens not present in the marrow donor. Paternal lymphocyte allografting occurred, as demonstrated by lymphocyte cytotoxicity testing, but both infants succumbed to graft-versus-host disease at the end of 3 weeks. Full text Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (3.5M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References. Images in this article Click on the image to see a larger version. These references are in PubMed. This may not be the complete list of references from this article. - Amos DB, Bach FH. Phenotypic expressions of the major histocompatibility locus in man (HL-A): leukocyte antigens and mixed leukocyte culture reactivity. J Exp Med. 1968 Oct 1;128(4):623–637. [PubMed]
- BATCHELOR JR, HOWARD JG. SYNERGIC AND ANTAGONISTIC EFFECTS OF ISOANTIBODY UPON GRAFT-VERSUS-HOST DISEASE. Transplantation. 1965 Mar;3:161–169. [PubMed]
- Bortin MM. A compendium of reported human bone marrow transplants. Transplantation. 1970 Jun;9(6):571–587. [PubMed]
- BOYDEN SV. The adsorption of proteins on erythrocytes treated with tannic acid and subsequent hemagglutination by antiprotein sera. J Exp Med. 1951 Feb;93(2):107–120. [PubMed]
- Buckley RH, Dees SC, O'Fallon WM. Serum immunoglobulins. I. Levels in normal children and in uncomplicated childhood allergy. Pediatrics. 1968 Mar;41(3):600–611. [PubMed]
- De Koning J, Van Bekkum DW, Dicke KA, Dooren LJ, Rádl J, Van Rood JJ. Transplantation of bone-marrow cells and fetal thymus in an infant with lymphopenic immunological deficiency. Lancet. 1969 Jun 21;1(7608):1223–1227. [PubMed]
- van Dicke KA Hooft JI, van Bekkum DW. The selective elimination of immunologically competent cells from bone marrow and lymphatic cell mixtures. II. Mouse spleen cell fractionation on a discontinuous albumin gradient. Transplantation. 1968 Jul;6(4):562–570. [PubMed]
- FORD CE, MICKLEM HS. The thymus and lymph-nodes in radiation chimaeras. Lancet. 1963 Feb 16;1(7277):359–362. [PubMed]
- French ME, Batchelor JR. Immunological enhancement of rat kidney grafts. Lancet. 1969 Nov 22;2(7630):1103–1106. [PubMed]
- FUDENBERG HH, HEREMANS JF, FRANKLIN EC. A hypothesis for the genetic control of synthesis of the gamma-globulins. Ann Inst Pasteur (Paris). 1963 Feb;104:155–168. [PubMed]
- Gatti RA, Meuwissen HJ, Allen HD, Hong R, Good RA. Immunological reconstitution of sex-linked lymphopenic immunological deficiency. Lancet. 1968 Dec 28;2(7583):1366–1369. [PubMed]
- Graw RG, Jr, Rogentine GN, Jr, Leventhal BG, Halterman RH, Berard C, Herzig GP, Yankee RA, Whang-Peng J, Krüger G, Henderson ES. Graft-versus-host reaction complicating HL-A matched bone-marrow transplantation. Lancet. 1970 Nov 21;2(7682):1053–1055. [PubMed]
- Hoyer JR, Cooper MD, Gabrielsen AE, Good RA. Lymphopenic forms of congenital immunologic deficiency diseases. Medicine (Baltimore). 1968 May;47(3):201–226. [PubMed]
- Kretschmer R, Jeannet M, Mereu TR, Kretschmer K, Winn H, Rosen FS. Hereditary thymic dysplasia: a graft-versus-host reaction induced by bone marrow cells with a partial 4a series histoincompatibility. Pediatr Res. 1969 Jan;3(1):34–40. [PubMed]
- Lucas ZJ. Pyrimidine nucleotide synthesis: regulatory control during transformation of lymphocytes in vitro. Science. 1967 Jun 2;156(779):1237–1240. [PubMed]
- McGregor DD. Bone marrow origin of immunologically competent lymphocytes in the rat. J Exp Med. 1968 May 1;127(5):953–966. [PubMed]
- Meuwissen HJ, Bach FH, Hong R, Good RA. Lymphocyte studies in congenital thymic dysplasia: The one-way stimulation test. J Pediatr. 1968 Feb;72(2):177–185. [PubMed]
- Miller ME, Schieken RM. Thymic dysplasia. A separable entity from "swiss agammaglobulinemia". Am J Med Sci. 1967 Jun;253(6):741–750. [PubMed]
- Rowe DS. Radioactive single radial diffusion: a method for increasing the sensitivity of immunochemical quantification of proteins in agar gel. Bull World Health Organ. 1969 Apr;40(4):613–616. [PubMed]
- Safford JW, Jr, Tokuda S. Suppression of the graft-versus-host reaction by passive immunization of donor against recipient antigens. Proc Soc Exp Biol Med. 1970 Feb;133(2):651–654. [PubMed]
- Storb R, Epstein RB, Graham TC, Thomas ED. Methotrexate regimens for control of graft-versus-host disease in dogs with allogeneic marrow grafts. Transplantation. 1970 Mar;9(3):240–246. [PubMed]
- Stuart FP, Saitoh T, Fitch FW. Rejection of renal allografts: specific immunologic suppression. Science. 1968 Jun 28;160(835):1463–1465. [PubMed]
- Zimmerman B, Feldman JD. Enhancing antibody. I. Bioassay for normal skin grafts. J Immunol. 1969 Feb;102(2):507–512. [PubMed]
|
