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Appl Environ Microbiol. Feb 1977; 33(2): 400–405.
PMCID: PMC170697

Isolation and molecular size of Clostridium botulinum type C toxin.


A procedure is described for the purification of hemagglutinin-free Clostridium botulinum type C toxin. The toxin was purified approximately 1,000-fold from the original culture supernatant in an overall yield of 60% to a final specific toxicity of 4.4 x 10(7) minimal lethal doses/mg of protein. The toxin had a molecular weight of 141,000 and consisted of a heavy and a light chain. The molecular weights of the subunits were approximately 98,000 and 53,000. When comparing the molecular size and composition of type C toxin to that of botulinum toxins of different types, some common features may be suggested; i.e., the toxin has a molecular weight between 141,000 to 160,000 and is comprised of a heavy and a light chain linked by disulfide bonds (or bond).

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Selected References

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  • Beers WH, Reich E. Isolation and characterization of Clostridium botulinum type B toxin. J Biol Chem. 1969 Aug 25;244(16):4473–4479. [PubMed]
  • Boroff DA, Fleck U. Statistical analysis of a rapid in vivo method for the titration of the toxin of Clostridium botulinum. J Bacteriol. 1966 Nov;92(5):1580–1581. [PMC free article] [PubMed]
  • DasGupta BR, Boroff DA. Separation of toxin and hemagglutinin from crystalline toxin of Clostridium botulinum type A by anion exchange chromatography and determination of their dimensions by gel filtration. J Biol Chem. 1968 Mar 10;243(5):1065–1072. [PubMed]
  • DasGupta BR, Sugiyama H. A common subunit structure in Clostridium botulinum type A, B and E toxins. Biochem Biophys Res Commun. 1972 Jul 11;48(1):108–112. [PubMed]
  • Dasgupta BR, Berry LJ, Boroff DA. Purification of Clostridium botulinum type A toxin. Biochim Biophys Acta. 1970 Aug 21;214(2):343–349. [PubMed]
  • Dasgupta BR, Boroff DA, Rothstein E. Chromatographic fractionation of the crystalline toxin of Clostridium botulinum type A. Biochem Biophys Res Commun. 1966 Mar 22;22(6):750–756. [PubMed]
  • DasGupta BR, Boroff DA, Cheong K. Isolation of chromatographically pure toxin of Clostridium botulinum type B. Biochem Biophys Res Commun. 1968 Sep 30;32(6):1057–1063. [PubMed]
  • Kitamura M, Sakaguchi S, Sakaguchi G. Purification and some properties of Clostridium botulinum type-E toxin. Biochim Biophys Acta. 1968 Oct 21;168(2):207–217. [PubMed]
  • Kitamura M, Sakaguchi S, Sakaguchi G. Significance of 12S toxin of Clostridium botulinum type E. J Bacteriol. 1969 Jun;98(3):1173–1178. [PMC free article] [PubMed]
  • Kitamura M, Sakaguchi G. Dissociation and reconstitution of 12-S toxin of Clostridium botulinum type E. Biochim Biophys Acta. 1969 Dec 23;194(2):564–571. [PubMed]
  • Knox J, Brown WP, Spero L. The role of sulfhydryl groups in the activity of type A botulinum toxin. Biochim Biophys Acta. 1970 Aug 21;214(2):350–354. [PubMed]
  • Lamanna C, McElroy OE, Eklund HW. The Purification and Crystallization of Clostridium botulinum Type A Toxin. Science. 1946 May 17;103(2681):613–614. [PubMed]
  • Livingston DM. Immunoaffinity chromatography of proteins. Methods Enzymol. 1974;34:723–731. [PubMed]
  • Oishi I, Sakaguchi G. Purification of Clostridium botuliunum type F progenitor toxin. Appl Microbiol. 1974 Dec;28(6):923–928. [PMC free article] [PubMed]
  • Ohishi I, Sakaguchi G. Molecular construction of Clostridium botulinum type F progenitor toxin. Appl Microbiol. 1975 Apr;29(4):444–447. [PMC free article] [PubMed]
  • STERNE M, WENTZEL LM. A new method for the large-scale production of high-titre botulinum formol-toxoid types C and D. J Immunol. 1950 Aug;65(2):175–183. [PubMed]
  • Syuto B, Kubo S. Purification and crystallization of Clostridium botulinum type C toxin. Jpn J Vet Res. 1972 Jun;20(1):19–30. [PubMed]
  • WAGMAN J. Isolation and sedimentation study of low molecular weight forms of type A Botulins toxin. Arch Biochem Biophys. 1954 May;50(1):104–112. [PubMed]
  • Weber K, Pringle JR, Osborn M. Measurement of molecular weights by electrophoresis on SDS-acrylamide gel. Methods Enzymol. 1972;26:3–27. [PubMed]
  • Yang KH, Sugiyama H. Purification and properties of Clostridium botulinum type F toxin. Appl Microbiol. 1975 May;29(5):598–603. [PMC free article] [PubMed]
  • YPHANSTIS DA. Rapid determination of molecular weights of peptides and preteins. Ann N Y Acad Sci. 1960 Aug 31;88:586–601. [PubMed]

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