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Philos Trans R Soc Lond B Biol Sci. Aug 29, 2004; 359(1448): 1269-75; discussion 1275, 1323-8.
PMCID: PMC1693409

The effect of water on protein dynamics.


Neutron diffraction and spectroscopy were applied to describe the hydration and dynamics of a soluble protein and a natural membrane from extreme halophilic Archaea. The quantitative dependence of protein motions on water activity was clearly illustrated, and it was established that a minimum hydration shell is required for the systems to access their functional resilience, i.e. a dynamics state that allows biological activity.

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  • Andreani C, Filabozzi A, Menzinger F, Desideri A, Deriu A, Di Cola D. Dynamics of hydrogen atoms in superoxide dismutase by quasielastic neutron scattering. Biophys J. 1995 Jun;68(6):2519–2523. [PMC free article] [PubMed]
  • Bicout DJ, Zaccai G. Protein flexibility from the dynamical transition: a force constant analysis. Biophys J. 2001 Mar;80(3):1115–1123. [PMC free article] [PubMed]
  • Bon C, Lehmann MS, Wilkinson C. Quasi-Laue neutron-diffraction study of the water arrangement in crystals of triclinic hen egg-white lysozyme. Acta Crystallogr D Biol Crystallogr. 1999 May;55(Pt 5):978–987. [PubMed]
  • Bon C, Dianoux AJ, Ferrand M, Lehmann MS. A model for water motion in crystals of lysozyme based on an incoherent quasielastic neutron-scattering study. Biophys J. 2002 Sep;83(3):1578–1588. [PMC free article] [PubMed]
  • Bonneté F, Madern D, Zaccaï G. Stability against denaturation mechanisms in halophilic malate dehydrogenase "adapt" to solvent conditions. J Mol Biol. 1994 Dec 9;244(4):436–447. [PubMed]
  • Cordone L, Ferrand M, Vitrano E, Zaccai G. Harmonic behavior of trehalose-coated carbon-monoxy-myoglobin at high temperature. Biophys J. 1999 Feb;76(2):1043–1047. [PMC free article] [PubMed]
  • Daniel RM, Finney JL, Réat V, Dunn R, Ferrand M, Smith JC. Enzyme dynamics and activity: time-scale dependence of dynamical transitions in glutamate dehydrogenase solution. Biophys J. 1999 Oct;77(4):2184–2190. [PMC free article] [PubMed]
  • Doster W, Cusack S, Petry W. Dynamical transition of myoglobin revealed by inelastic neutron scattering. Nature. 1989 Feb 23;337(6209):754–756. [PubMed]
  • Ferrand M, Dianoux AJ, Petry W, Zaccaï G. Thermal motions and function of bacteriorhodopsin in purple membranes: effects of temperature and hydration studied by neutron scattering. Proc Natl Acad Sci U S A. 1993 Oct 15;90(20):9668–9672. [PMC free article] [PubMed]
  • Fitter J. The temperature dependence of internal molecular motions in hydrated and dry alpha-amylase: the role of hydration water in the dynamical transition of proteins. Biophys J. 1999 Feb;76(2):1034–1042. [PMC free article] [PubMed]
  • Gabel Frank, Bicout Dominique, Lehnert Ursula, Tehei Moeava, Weik Martin, Zaccai Giuseppe. Protein dynamics studied by neutron scattering. Q Rev Biophys. 2002 Nov;35(4):327–367. [PubMed]
  • Grigorieff N, Ceska TA, Downing KH, Baldwin JM, Henderson R. Electron-crystallographic refinement of the structure of bacteriorhodopsin. J Mol Biol. 1996 Jun 14;259(3):393–421. [PubMed]
  • Irimia Adriana, Ebel Christine, Madern Dominique, Richard Stéphane B, Cosenza Lawrence W, Zaccaï Giuseppe, Vellieux Frédéric M D. The Oligomeric states of Haloarcula marismortui malate dehydrogenase are modulated by solvent components as shown by crystallographic and biochemical studies. J Mol Biol. 2003 Feb 21;326(3):859–873. [PubMed]
  • Lehnert U, Réat V, Weik M, Zaccaï G, Pfister C. Thermal motions in bacteriorhodopsin at different hydration levels studied by neutron scattering: correlation with kinetics and light-induced conformational changes. Biophys J. 1998 Oct;75(4):1945–1952. [PMC free article] [PubMed]
  • Madern D, Ebel C, Zaccai G. Halophilic adaptation of enzymes. Extremophiles. 2000 Apr;4(2):91–98. [PubMed]
  • Merzel Franci, Smith Jeremy C. Is the first hydration shell of lysozyme of higher density than bulk water? Proc Natl Acad Sci U S A. 2002 Apr 16;99(8):5378–5383. [PMC free article] [PubMed]
  • Oesterhelt D. The structure and mechanism of the family of retinal proteins from halophilic archaea. Curr Opin Struct Biol. 1998 Aug;8(4):489–500. [PubMed]
  • Paciaroni A, Stroppolo ME, Arcangeli C, Bizzarri AR, Desideri A, Cannistraro S. Incoherent neutron scattering of copper azurin: a comparison with molecular dynamics simulation results. Eur Biophys J. 1999;28(6):447–456. [PubMed]
  • Papadopoulos G, Dencher NA, Zaccai G, Büldt G. Water molecules and exchangeable hydrogen ions at the active centre of bacteriorhodopsin localized by neutron diffraction. Elements of the proton pathway? J Mol Biol. 1990 Jul 5;214(1):15–19. [PubMed]
  • Parak F, Knapp EW, Kucheida D. Protein dynamics. Mössbauer spectroscopy on deoxymyoglobin crystals. J Mol Biol. 1982 Oct 15;161(1):177–194. [PubMed]
  • Réat V, Dunn R, Ferrand M, Finney JL, Daniel RM, Smith JC. Solvent dependence of dynamic transitions in protein solutions. Proc Natl Acad Sci U S A. 2000 Aug 29;97(18):9961–9966. [PMC free article] [PubMed]
  • Svergun DI, Richard S, Koch MH, Sayers Z, Kuprin S, Zaccai G. Protein hydration in solution: experimental observation by x-ray and neutron scattering. Proc Natl Acad Sci U S A. 1998 Mar 3;95(5):2267–2272. [PMC free article] [PubMed]
  • Tehei Moeava, Franzetti Bruno, Maurel Marie-Christine, Vergne Jacques, Hountondji Codjo, Zaccai Giuseppe. The search for traces of life: the protective effect of salt on biological macromolecules. Extremophiles. 2002 Oct;6(5):427–430. [PubMed]
  • Tehei Moeava, Franzetti Bruno, Madern Dominique, Ginzburg Margaret, Ginzburg Ben Z, Giudici-Orticoni Marie-Thérèse, Bruschi Mireille, Zaccai Giuseppe. Adaptation to extreme environments: macromolecular dynamics in bacteria compared in vivo by neutron scattering. EMBO Rep. 2004 Jan;5(1):66–70. [PMC free article] [PubMed]
  • Tournier Alexander L, Xu Jiancong, Smith Jeremy C. Translational hydration water dynamics drives the protein glass transition. Biophys J. 2003 Sep;85(3):1871–1875. [PMC free article] [PubMed]
  • Zaccai G. Structure and hydration of purple membranes in different conditions. J Mol Biol. 1987 Apr 5;194(3):569–572. [PubMed]
  • Zaccai G. How soft is a protein? A protein dynamics force constant measured by neutron scattering. Science. 2000 Jun 2;288(5471):1604–1607. [PubMed]
  • Zaccai G, Gilmore DJ. Areas of hydration in the purple membrane of Halobacterium halobium: a neutron diffraction study. J Mol Biol. 1979 Aug 5;132(2):181–191. [PubMed]

Articles from Philosophical Transactions of the Royal Society B: Biological Sciences are provided here courtesy of The Royal Society


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