• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of transbhomepageaboutsubmitalertseditorial board
Philos Trans R Soc Lond B Biol Sci. Aug 29, 2004; 359(1448): 1269-75; discussion 1275, 1323-8.
PMCID: PMC1693409

The effect of water on protein dynamics.

Abstract

Neutron diffraction and spectroscopy were applied to describe the hydration and dynamics of a soluble protein and a natural membrane from extreme halophilic Archaea. The quantitative dependence of protein motions on water activity was clearly illustrated, and it was established that a minimum hydration shell is required for the systems to access their functional resilience, i.e. a dynamics state that allows biological activity.

Full Text

The Full Text of this article is available as a PDF (372K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Andreani C, Filabozzi A, Menzinger F, Desideri A, Deriu A, Di Cola D. Dynamics of hydrogen atoms in superoxide dismutase by quasielastic neutron scattering. Biophys J. 1995 Jun;68(6):2519–2523. [PMC free article] [PubMed]
  • Bicout DJ, Zaccai G. Protein flexibility from the dynamical transition: a force constant analysis. Biophys J. 2001 Mar;80(3):1115–1123. [PMC free article] [PubMed]
  • Bon C, Lehmann MS, Wilkinson C. Quasi-Laue neutron-diffraction study of the water arrangement in crystals of triclinic hen egg-white lysozyme. Acta Crystallogr D Biol Crystallogr. 1999 May;55(Pt 5):978–987. [PubMed]
  • Bon C, Dianoux AJ, Ferrand M, Lehmann MS. A model for water motion in crystals of lysozyme based on an incoherent quasielastic neutron-scattering study. Biophys J. 2002 Sep;83(3):1578–1588. [PMC free article] [PubMed]
  • Bonneté F, Madern D, Zaccaï G. Stability against denaturation mechanisms in halophilic malate dehydrogenase "adapt" to solvent conditions. J Mol Biol. 1994 Dec 9;244(4):436–447. [PubMed]
  • Cordone L, Ferrand M, Vitrano E, Zaccai G. Harmonic behavior of trehalose-coated carbon-monoxy-myoglobin at high temperature. Biophys J. 1999 Feb;76(2):1043–1047. [PMC free article] [PubMed]
  • Daniel RM, Finney JL, Réat V, Dunn R, Ferrand M, Smith JC. Enzyme dynamics and activity: time-scale dependence of dynamical transitions in glutamate dehydrogenase solution. Biophys J. 1999 Oct;77(4):2184–2190. [PMC free article] [PubMed]
  • Doster W, Cusack S, Petry W. Dynamical transition of myoglobin revealed by inelastic neutron scattering. Nature. 1989 Feb 23;337(6209):754–756. [PubMed]
  • Ferrand M, Dianoux AJ, Petry W, Zaccaï G. Thermal motions and function of bacteriorhodopsin in purple membranes: effects of temperature and hydration studied by neutron scattering. Proc Natl Acad Sci U S A. 1993 Oct 15;90(20):9668–9672. [PMC free article] [PubMed]
  • Fitter J. The temperature dependence of internal molecular motions in hydrated and dry alpha-amylase: the role of hydration water in the dynamical transition of proteins. Biophys J. 1999 Feb;76(2):1034–1042. [PMC free article] [PubMed]
  • Gabel Frank, Bicout Dominique, Lehnert Ursula, Tehei Moeava, Weik Martin, Zaccai Giuseppe. Protein dynamics studied by neutron scattering. Q Rev Biophys. 2002 Nov;35(4):327–367. [PubMed]
  • Grigorieff N, Ceska TA, Downing KH, Baldwin JM, Henderson R. Electron-crystallographic refinement of the structure of bacteriorhodopsin. J Mol Biol. 1996 Jun 14;259(3):393–421. [PubMed]
  • Irimia Adriana, Ebel Christine, Madern Dominique, Richard Stéphane B, Cosenza Lawrence W, Zaccaï Giuseppe, Vellieux Frédéric M D. The Oligomeric states of Haloarcula marismortui malate dehydrogenase are modulated by solvent components as shown by crystallographic and biochemical studies. J Mol Biol. 2003 Feb 21;326(3):859–873. [PubMed]
  • Lehnert U, Réat V, Weik M, Zaccaï G, Pfister C. Thermal motions in bacteriorhodopsin at different hydration levels studied by neutron scattering: correlation with kinetics and light-induced conformational changes. Biophys J. 1998 Oct;75(4):1945–1952. [PMC free article] [PubMed]
  • Madern D, Ebel C, Zaccai G. Halophilic adaptation of enzymes. Extremophiles. 2000 Apr;4(2):91–98. [PubMed]
  • Merzel Franci, Smith Jeremy C. Is the first hydration shell of lysozyme of higher density than bulk water? Proc Natl Acad Sci U S A. 2002 Apr 16;99(8):5378–5383. [PMC free article] [PubMed]
  • Oesterhelt D. The structure and mechanism of the family of retinal proteins from halophilic archaea. Curr Opin Struct Biol. 1998 Aug;8(4):489–500. [PubMed]
  • Paciaroni A, Stroppolo ME, Arcangeli C, Bizzarri AR, Desideri A, Cannistraro S. Incoherent neutron scattering of copper azurin: a comparison with molecular dynamics simulation results. Eur Biophys J. 1999;28(6):447–456. [PubMed]
  • Papadopoulos G, Dencher NA, Zaccai G, Büldt G. Water molecules and exchangeable hydrogen ions at the active centre of bacteriorhodopsin localized by neutron diffraction. Elements of the proton pathway? J Mol Biol. 1990 Jul 5;214(1):15–19. [PubMed]
  • Parak F, Knapp EW, Kucheida D. Protein dynamics. Mössbauer spectroscopy on deoxymyoglobin crystals. J Mol Biol. 1982 Oct 15;161(1):177–194. [PubMed]
  • Réat V, Dunn R, Ferrand M, Finney JL, Daniel RM, Smith JC. Solvent dependence of dynamic transitions in protein solutions. Proc Natl Acad Sci U S A. 2000 Aug 29;97(18):9961–9966. [PMC free article] [PubMed]
  • Svergun DI, Richard S, Koch MH, Sayers Z, Kuprin S, Zaccai G. Protein hydration in solution: experimental observation by x-ray and neutron scattering. Proc Natl Acad Sci U S A. 1998 Mar 3;95(5):2267–2272. [PMC free article] [PubMed]
  • Tehei Moeava, Franzetti Bruno, Maurel Marie-Christine, Vergne Jacques, Hountondji Codjo, Zaccai Giuseppe. The search for traces of life: the protective effect of salt on biological macromolecules. Extremophiles. 2002 Oct;6(5):427–430. [PubMed]
  • Tehei Moeava, Franzetti Bruno, Madern Dominique, Ginzburg Margaret, Ginzburg Ben Z, Giudici-Orticoni Marie-Thérèse, Bruschi Mireille, Zaccai Giuseppe. Adaptation to extreme environments: macromolecular dynamics in bacteria compared in vivo by neutron scattering. EMBO Rep. 2004 Jan;5(1):66–70. [PMC free article] [PubMed]
  • Tournier Alexander L, Xu Jiancong, Smith Jeremy C. Translational hydration water dynamics drives the protein glass transition. Biophys J. 2003 Sep;85(3):1871–1875. [PMC free article] [PubMed]
  • Zaccai G. Structure and hydration of purple membranes in different conditions. J Mol Biol. 1987 Apr 5;194(3):569–572. [PubMed]
  • Zaccai G. How soft is a protein? A protein dynamics force constant measured by neutron scattering. Science. 2000 Jun 2;288(5471):1604–1607. [PubMed]
  • Zaccai G, Gilmore DJ. Areas of hydration in the purple membrane of Halobacterium halobium: a neutron diffraction study. J Mol Biol. 1979 Aug 5;132(2):181–191. [PubMed]

Articles from Philosophical Transactions of the Royal Society B: Biological Sciences are provided here courtesy of The Royal Society

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...