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Proc Biol Sci. Feb 7, 2003; 270(1512): 313–321.
PMCID: PMC1691236

Biological identifications through DNA barcodes.


Although much biological research depends upon species diagnoses, taxonomic expertise is collapsing. We are convinced that the sole prospect for a sustainable identification capability lies in the construction of systems that employ DNA sequences as taxon 'barcodes'. We establish that the mitochondrial gene cytochrome c oxidase I (COI) can serve as the core of a global bioidentification system for animals. First, we demonstrate that COI profiles, derived from the low-density sampling of higher taxonomic categories, ordinarily assign newly analysed taxa to the appropriate phylum or order. Second, we demonstrate that species-level assignments can be obtained by creating comprehensive COI profiles. A model COI profile, based upon the analysis of a single individual from each of 200 closely allied species of lepidopterans, was 100% successful in correctly identifying subsequent specimens. When fully developed, a COI identification system will provide a reliable, cost-effective and accessible solution to the current problem of species identification. Its assembly will also generate important new insights into the diversification of life and the rules of molecular evolution.

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Selected References

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  • Avise JC, Walker D. Species realities and numbers in sexual vertebrates: perspectives from an asexually transmitted genome. Proc Natl Acad Sci U S A. 1999 Feb 2;96(3):992–995. [PMC free article] [PubMed]
  • Cox AJ, Hebert PD. Colonization, extinction, and phylogeographic patterning in a freshwater crustacean. Mol Ecol. 2001 Feb;10(2):371–386. [PubMed]
  • Doyle JJ, Gaut BS. Evolution of genes and taxa: a primer. Plant Mol Biol. 2000 Jan;42(1):1–23. [PubMed]
  • Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol Mar Biol Biotechnol. 1994 Oct;3(5):294–299. [PubMed]
  • Godfray HC, Lewis T, Memmott J. Studying insect diversity in the tropics. Philos Trans R Soc Lond B Biol Sci. 1999 Nov 29;354(1391):1811–1824. [PMC free article] [PubMed]
  • Hamels S, Gala JL, Dufour S, Vannuffel P, Zammatteo N, Remacle J. Consensus PCR and microarray for diagnosis of the genus Staphylococcus, species, and methicillin resistance. Biotechniques. 2001 Dec;31(6):1364–1372. [PubMed]
  • Knowlton N, Weigt LA. New dates and new rates for divergence across the Isthmus of Panama. Proc Biol Sci. 1998 Dec 7;265(1412):2257–2257. [PMC free article]
  • Kumar S, Gadagkar SR. Efficiency of the neighbor-joining method in reconstructing deep and shallow evolutionary relationships in large phylogenies. J Mol Evol. 2000 Dec;51(6):544–553. [PubMed]
  • Kurtzman CP. Molecular taxonomy of the yeasts. Yeast. 1994 Dec;10(13):1727–1740. [PubMed]
  • Lynch M, Jarrell PE. A method for calibrating molecular clocks and its application to animal mitochondrial DNA. Genetics. 1993 Dec;135(4):1197–1208. [PMC free article] [PubMed]
  • Mardulyn P, Whitfield JB. Phylogenetic signal in the COI, 16S, and 28S genes for inferring relationships among genera of Microgastrinae (Hymenoptera; Braconidae): evidence of a high diversification rate in this group of parasitoids. Mol Phylogenet Evol. 1999 Aug;12(3):282–294. [PubMed]
  • Novotny Vojtech, Basset Yves, Miller Scott E, Weiblen George D, Bremer Birgitta, Cizek Lukas, Drozd Pavel. Low host specificity of herbivorous insects in a tropical forest. Nature. 2002 Apr 25;416(6883):841–844. [PubMed]
  • Pace NR. A molecular view of microbial diversity and the biosphere. Science. 1997 May 2;276(5313):734–740. [PubMed]
  • Saccone C, De Giorgi C, Gissi C, Pesole G, Reyes A. Evolutionary genomics in Metazoa: the mitochondrial DNA as a model system. Gene. 1999 Sep 30;238(1):195–209. [PubMed]
  • Simmons RB, Weller SJ. Utility and evolution of cytochrome b in insects. Mol Phylogenet Evol. 2001 Aug;20(2):196–210. [PubMed]
  • Trewick SA. Mitochondrial DNA sequences support allozyme evidence for cryptic radiation of New Zealand Peripatoides (Onychophora). Mol Ecol. 2000 Mar;9(3):269–281. [PubMed]
  • Vincent S, Vian JM, Carlotti MP. Partial sequencing of the cytochrome oxydase b subunit gene I: a tool for the identification of European species of blow flies for postmortem interval estimation. J Forensic Sci. 2000 Jul;45(4):820–823. [PubMed]
  • Wares JP, Cunningham CW. Phylogeography and historical ecology of the North Atlantic intertidal. Evolution. 2001 Dec;55(12):2455–2469. [PubMed]
  • Williams ST, Knowlton N. Mitochondrial pseudogenes are pervasive and often insidious in the snapping shrimp genus Alpheus. Mol Biol Evol. 2001 Aug;18(8):1484–1493. [PubMed]
  • Zhang DX, Hewitt GM. Assessment of the universality and utility of a set of conserved mitochondrial COI primers in insects. Insect Mol Biol. 1997 May;6(2):143–150. [PubMed]

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