• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of procbhomepageaboutsubmitalertseditorial board
Proc Biol Sci. Sep 22, 1999; 266(1431): 1905–1912.
PMCID: PMC1690223

X chromosome DNA variation in Drosophila virilis.

Abstract

Comparisons of polymorphism patterns between distantly related species are essential in order to determine their generality. However, most work on the genus Drosophila has been done only with species of the subgenus Sophophora. In the present work, we have sequenced one intron and surrounding coding sequences of 6 X-linked genes (chorion protein s36, elav, fused, runt, suppressor of sable and zeste) from 21 strains of wild-type Drosophila virilis (subgenus Drosophila). From these data, we have estimated the average level of DNA polymorphism, inferred the effective population size and population structure of this species, and compared the results with those obtained for other Drosophila species. There is no reduction in variation at two loci close to the centromeric heterochromatin, in contrast to Drosophila melanogaster.

Full Text

The Full Text of this article is available as a PDF (146K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Aguadé M, Miyashita N, Langley CH. Restriction-map variation at the zeste-tko region in natural populations of Drosophila melanogaster. Mol Biol Evol. 1989 Mar;6(2):123–130. [PubMed]
  • Bauer VL, Aquadro CF. Rates of DNA sequence evolution are not sex-biased in Drosophila melanogaster and D. simulans. Mol Biol Evol. 1997 Dec;14(12):1252–1257. [PubMed]
  • Begun DJ, Aquadro CF. Molecular population genetics of the distal portion of the X chromosome in Drosophila: evidence for genetic hitchhiking of the yellow-achaete region. Genetics. 1991 Dec;129(4):1147–1158. [PMC free article] [PubMed]
  • Begun DJ, Aquadro CF. Levels of naturally occurring DNA polymorphism correlate with recombination rates in D. melanogaster. Nature. 1992 Apr 9;356(6369):519–520. [PubMed]
  • Berry AJ, Ajioka JW, Kreitman M. Lack of polymorphism on the Drosophila fourth chromosome resulting from selection. Genetics. 1991 Dec;129(4):1111–1117. [PMC free article] [PubMed]
  • Charlesworth B. Background selection and patterns of genetic diversity in Drosophila melanogaster. Genet Res. 1996 Oct;68(2):131–149. [PubMed]
  • Charlesworth D, Charlesworth B. Sequence variation: looking for effects of genetic linkage. Curr Biol. 1998 Sep 10;8(18):R658–R661. [PubMed]
  • Comeron JM, Kreitman M, Aguadé M. Natural selection on synonymous sites is correlated with gene length and recombination in Drosophila. Genetics. 1999 Jan;151(1):239–249. [PMC free article] [PubMed]
  • Dvorák J, Luo MC, Yang ZL. Restriction fragment length polymorphism and divergence in the genomic regions of high and low recombination in self-fertilizing and cross-fertilizing aegilops species. Genetics. 1998 Jan;148(1):423–434. [PMC free article] [PubMed]
  • Fu YX, Li WH. Statistical tests of neutrality of mutations. Genetics. 1993 Mar;133(3):693–709. [PMC free article] [PubMed]
  • Hanks SK, Quinn AM, Hunter T. The protein kinase family: conserved features and deduced phylogeny of the catalytic domains. Science. 1988 Jul 1;241(4861):42–52. [PubMed]
  • Hilton H, Hey J. DNA sequence variation at the period locus reveals the history of species and speciation events in the Drosophila virilis group. Genetics. 1996 Nov;144(3):1015–1025. [PMC free article] [PubMed]
  • Hudson RR, Kreitman M, Aguadé M. A test of neutral molecular evolution based on nucleotide data. Genetics. 1987 May;116(1):153–159. [PMC free article] [PubMed]
  • Hudson RR, Bailey K, Skarecky D, Kwiatowski J, Ayala FJ. Evidence for positive selection in the superoxide dismutase (Sod) region of Drosophila melanogaster. Genetics. 1994 Apr;136(4):1329–1340. [PMC free article] [PubMed]
  • Kliman RM, Hey J. Reduced natural selection associated with low recombination in Drosophila melanogaster. Mol Biol Evol. 1993 Nov;10(6):1239–1258. [PubMed]
  • Kokoza EB, Kozlova T Iu, Umbetova G Kh, Dubrovskii EB, Pirrotta V, Zhimulev IF. Molekuliaeno-tsitogeneticheskii analiz diska 10A1-2 X-khromosomy Drosophila melanogaster. Genetika. 1990 Aug;26(8):1361–1369. [PubMed]
  • Konsolaki M, Komitopoulou K, Tolias PP, King DL, Swimmer C, Kafatos FC. The chorion genes of the medfly, Ceratitis capitata, I: Structural and regulatory conservation of the s36 gene relative to two Drosophila species. Nucleic Acids Res. 1990 Apr 11;18(7):1731–1737. [PMC free article] [PubMed]
  • Kraft T, Säll T, Magnusson-Rading I, Nilsson NO, Halldén C. Positive correlation between recombination rates and levels of genetic variation in natural populations of sea beet (Beta vulgaris subsp. maritima). Genetics. 1998 Nov;150(3):1239–1244. [PMC free article] [PubMed]
  • Kress H. The salivary gland chromosomes of Drosophila virilis: a cytological map, pattern of transcription and aspects of chromosome evolution. Chromosoma. 1993 Dec;102(10):734–742. [PubMed]
  • Kwiatowski J, Skarecky D, Bailey K, Ayala FJ. Phylogeny of Drosophila and related genera inferred from the nucleotide sequence of the Cu,Zn Sod gene. J Mol Evol. 1994 May;38(5):443–454. [PubMed]
  • Lozovskaya ER, Petrov DA, Hartl DL. A combined molecular and cytogenetic approach to genome evolution in Drosophila using large-fragment DNA cloning. Chromosoma. 1993 Mar;102(4):253–266. [PubMed]
  • McVean GA, Vieira J. The evolution of codon preferences in Drosophila: a maximum-likelihood approach to parameter estimation and hypothesis testing. J Mol Evol. 1999 Jul;49(1):63–75. [PubMed]
  • Moriyama EN, Powell JR. Intraspecific nuclear DNA variation in Drosophila. Mol Biol Evol. 1996 Jan;13(1):261–277. [PubMed]
  • Nachman MW. Patterns of DNA variability at X-linked loci in Mus domesticus. Genetics. 1997 Nov;147(3):1303–1316. [PMC free article] [PubMed]
  • Nachman MW, Bauer VL, Crowell SL, Aquadro CF. DNA variability and recombination rates at X-linked loci in humans. Genetics. 1998 Nov;150(3):1133–1141. [PMC free article] [PubMed]
  • Nurminsky DI, Moriyama EN, Lozovskaya ER, Hartl DL. Molecular phylogeny and genome evolution in the Drosophila virilis species group: duplications of the alcohol dehydrogenase gene. Mol Biol Evol. 1996 Jan;13(1):132–149. [PubMed]
  • Rozas J, Rozas R. DnaSP version 2.0: a novel software package for extensive molecular population genetics analysis. Comput Appl Biosci. 1997 Jun;13(3):307–311. [PubMed]
  • Russo CA, Takezaki N, Nei M. Molecular phylogeny and divergence times of drosophilid species. Mol Biol Evol. 1995 May;12(3):391–404. [PubMed]
  • Stephan W, Langley CH. Molecular genetic variation in the centromeric region of the X chromosome in three Drosophila ananassae populations. I. Contrasts between the vermilion and forked loci. Genetics. 1989 Jan;121(1):89–99. [PMC free article] [PubMed]
  • Stephan W, Langley CH. DNA polymorphism in lycopersicon and crossing-over per physical length. Genetics. 1998 Dec;150(4):1585–1593. [PMC free article] [PubMed]
  • Tajima F. Statistical method for testing the neutral mutation hypothesis by DNA polymorphism. Genetics. 1989 Nov;123(3):585–595. [PMC free article] [PubMed]
  • Thérond P, Alves G, Limbourg-Bouchon B, Tricoire H, Guillemet E, Brissard-Zahraoui J, Lamour-Isnard C, Busson D. Functional domains of fused, a serine-threonine kinase required for signaling in Drosophila. Genetics. 1996 Apr;142(4):1181–1198. [PMC free article] [PubMed]
  • Tominaga H, Narise S. Sequence evolution of the Gpdh gene in the Drosophila virilis species group. Genetica. 1995;96(3):293–302. [PubMed]
  • Vieira J, Vieira CP, Hartl DL, Lozovskaya ER. A framework physical map of Drosophila virilis based on P1 clones: applications in genome evolution. Chromosoma. 1997 Jul;106(2):99–107. [PubMed]
  • Watterson GA. On the number of segregating sites in genetical models without recombination. Theor Popul Biol. 1975 Apr;7(2):256–276. [PubMed]
  • Wright F. The 'effective number of codons' used in a gene. Gene. 1990 Mar 1;87(1):23–29. [PubMed]

Articles from Proceedings of the Royal Society B: Biological Sciences are provided here courtesy of The Royal Society

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...