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Appl Environ Microbiol. Sep 1997; 63(9): 3724–3728.
PMCID: PMC168680

Enzymatic removal and disinfection of bacterial biofilms.

Abstract

Model biofilms of Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas fluorescens, and Pseudomonas aeruginosa were made on steel and polypropylene substrata. Plaque-resembling biofilms of Streptococcus mutans, Actinomyces viscosus, and Fusobacterium nucleatum were made on saliva-coated hydroxyapatite. The activity of enzymes against bacterial cells in biofilm was measured by fluorescence microscopy and an indirect conductance test in which evolution of carbon dioxide was measured. Glucose oxidase combined with lactoperoxidase was bactericidal against biofilm bacteria but did not remove the biofilm from the substrata. A complex mixture of polysaccharide-hydrolyzing enzymes was able to remove bacterial biofilm from steel and polypropylene substrata but did not have a significant bactericidal activity. Combining oxidoreductases with polysaccharide-hydrolyzing enzymes resulted in bactericidal activity as well as removal of the biofilm.

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Selected References

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  • Bolton FJ. An investigation of indirect conductimetry for detection of some food-borne bacteria. J Appl Bacteriol. 1990 Nov;69(5):655–661. [PubMed]
  • Brown ML, Aldrich HC, Gauthier JJ. Relationship between glycocalyx and povidone-iodine resistance in Pseudomonas aeruginosa (ATCC 27853) biofilms. Appl Environ Microbiol. 1995 Jan;61(1):187–193. [PMC free article] [PubMed]
  • Costerton JW, Lewandowski Z, Caldwell DE, Korber DR, Lappin-Scott HM. Microbial biofilms. Annu Rev Microbiol. 1995;49:711–745. [PubMed]
  • Dionysius DA, Grieve PA, Vos AC. Studies on the lactoperoxidase system: reaction kinetics and antibacterial activity using two methods for hydrogen peroxide generation. J Appl Bacteriol. 1992 Feb;72(2):146–153. [PubMed]
  • Gram L, Wedell-Neergaard C, Huss HH. The bacteriology of fresh and spoiling Lake Victorian Nile perch (Lates niloticus). Int J Food Microbiol. 1990 May;10(3-4):303–316. [PubMed]
  • Johansen C, Gill T, Gram L. Antibacterial effect of protamine assayed by impedimetry. J Appl Bacteriol. 1995 Mar;78(3):297–303. [PubMed]
  • Khardori N, Yassien M, Wilson K. Tolerance of Staphylococcus epidermidis grown from indwelling vascular catheters to antimicrobial agents. J Ind Microbiol. 1995 Sep;15(3):148–151. [PubMed]
  • Larragoiti ND, Diamos ME, Simmons PA, Edrington TB. A comparative study of techniques for decreasing contact lens storage case contamination. J Am Optom Assoc. 1994 Mar;65(3):161–163. [PubMed]
  • MILLER GL, GOLDER RH. Buffers of pH 2 to 12 for use in electrophoresis. Arch Biochem. 1950 Dec;29(2):420–423. [PubMed]
  • Passerini L, Lam K, Costerton JW, King EG. Biofilms on indwelling vascular catheters. Crit Care Med. 1992 May;20(5):665–673. [PubMed]
  • Rodriguez GG, Phipps D, Ishiguro K, Ridgway HF. Use of a fluorescent redox probe for direct visualization of actively respiring bacteria. Appl Environ Microbiol. 1992 Jun;58(6):1801–1808. [PMC free article] [PubMed]
  • Roger V, Tenovuo J, Lenander-Lumikari M, Söderling E, Vilja P. Lysozyme and lactoperoxidase inhibit the adherence of Streptococcus mutans NCTC 10449 (serotype c) to saliva-treated hydroxyapatite in vitro. Caries Res. 1994;28(6):421–428. [PubMed]
  • Schein OD, Glynn RJ, Poggio EC, Seddon JM, Kenyon KR. The relative risk of ulcerative keratitis among users of daily-wear and extended-wear soft contact lenses. A case-control study. Microbial Keratitis Study Group. N Engl J Med. 1989 Sep 21;321(12):773–778. [PubMed]
  • Sutherland IW. Polysaccharide lyases. FEMS Microbiol Rev. 1995 Jul;16(4):323–347. [PubMed]
  • Thomas EL, Pera KA, Smith KW, Chwang AK. Inhibition of Streptococcus mutans by the lactoperoxidase antimicrobial system. Infect Immun. 1983 Feb;39(2):767–778. [PMC free article] [PubMed]

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