• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of ajhgLink to Publisher's site
Am J Hum Genet. Jul 1983; 35(4): 565–572.
PMCID: PMC1685723

Linkage of the evolutionarily-related serum albumin and alpha-fetoprotein genes within q11-22 of human chromosome 4.

Abstract

Albumin and alpha-fetoprotein are structurally related serum proteins, having a similar gene structure and, conceivably, a common evolutionary origin. To test their relative arrangement in the human genome, the serum albumin and alpha-fetoprotein genes were mapped by in situ hybridization of cloned human albumin or alpha-fetoprotein cDNA to human mitotic chromosome preparations. Analysis of cells hybridized with the serum albumin probe showed that 39% of cells exhibited grains on the proximal portion of the long arm of chromosome 4 (bands q11-22), with these grains comprising 30% of all labeled sites throughout these mitoses. Similarly, in cells hybridized with the alpha-fetoprotein probe, 39% of cells were observed to contain silver grains on 4q11-22, these grains constituting 20% of all labeled sites in these cells. These results demonstrate chromosomal localization and linkage of the serum albumin and alpha-fetoprotein genes within bands q11-22 of the long arm of human chromosome 4.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.1M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Gitlin D, Perricelli A. Synthesis of serum albumin, prealbumin, alpha-foetoprotein, alpha-1-antitrypsin and transferrin by the human yolk sac. Nature. 1970 Dec 5;228(5275):995–997. [PubMed]
  • Ruoslahti E, Pihko H, Seppälä M. Alpha-fetoprotein: immunochemical purification and chemical properties. Expression in normal state and in malignant and non-malignant liver disease. Transplant Rev. 1974;20(0):38–60. [PubMed]
  • Gitlin D. Normal biology of alpha-fetoprotein. Ann N Y Acad Sci. 1975 Aug 22;259:7–16. [PubMed]
  • Ruoslahti E, Terry WD. alpha foetoprotein and serum albumin show sequence homology. Nature. 1976 Apr 29;260(5554):804–805. [PubMed]
  • Sargent TD, Yang M, Bonner J. Nucleotide sequence of cloned rat serum albumin messenger RNA. Proc Natl Acad Sci U S A. 1981 Jan;78(1):243–246. [PMC free article] [PubMed]
  • Jagodzinski LL, Sargent TD, Yang M, Glackin C, Bonner J. Sequence homology between RNAs encoding rat alpha-fetoprotein and rat serum albumin. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3521–3525. [PMC free article] [PubMed]
  • Law SW, Dugaiczyk A. Homology between the primary structure of alpha-fetoprotein, deduced from a complete cDNA sequence, and serum albumin. Nature. 1981 May 21;291(5812):201–205. [PubMed]
  • Gorin MB, Cooper DL, Eiferman F, van de Rijn P, Tilghman SM. The evolution of alpha-fetoprotein and albumin. I. A comparison of the primary amino acid sequences of mammalian alpha-fetoprotein and albumin. J Biol Chem. 1981 Feb 25;256(4):1954–1959. [PubMed]
  • Dugaiczyk A, Law SW, Dennison OE. Nucleotide sequence and the encoded amino acids of human serum albumin mRNA. Proc Natl Acad Sci U S A. 1982 Jan;79(1):71–75. [PMC free article] [PubMed]
  • Beattie WG, Dugaiczyk A. Structure and evolution of human alpha-fetoprotein deduced from partial sequence of cloned cDNA. Gene. 1982 Dec;20(3):415–422. [PubMed]
  • Brown JR. Structural origins of mammalian albumin. Fed Proc. 1976 Aug;35(10):2141–2144. [PubMed]
  • Hawkins JW, Dugaiczyk A. The human serum albumin gene: structure of a unique locus. Gene. 1982 Jul-Aug;19(1):55–58. [PubMed]
  • Bootsma D, McAlpine PJ. Report of the committee on the genetic constitution of chromosomes 2, 3, 4, and 5. Cytogenet Cell Genet. 1979;25(1-4):21–31. [PubMed]
  • Abelev GI. Alpha-fetoprotein as a marker of embryo-specific differentiations in normal and tumor tissues. Transplant Rev. 1974;20(0):3–37. [PubMed]
  • McIntire KR, Waldmann TA, Moertel CG, Go VL. Serum alpha-fetoprotein in patients with neoplasms of the gastrointestinal tract. Cancer Res. 1975 Apr;35(4):991–996. [PubMed]
  • Sell S, Becker FF, Leffert HL, Watabe L. Expression of an oncodevelopmental gene product (alpha-fetoprotein) during fetal development and adult oncogenesis. Cancer Res. 1976 Nov;36(11 Pt 2):4239–4249. [PubMed]
  • Bélanger L, Bélanger M, Prive L, Larochelle J, Tremblay M, Aubin G. Tyrosinémie héréditaire et alpha-1-foetoprotéine. I. Intérêt clinique de l'alpha-foeto-protéine dans la tyrosinémie héréditaire. Pathol Biol (Paris) 1973 May;21(5):449–455. [PubMed]
  • Bélanger L. Tyrosinémie héréditaire et alpha-1-foetoprotéine. II. Recherche tissulaire comparée de l'alpha-foetoprotéine dans deux cas de tyrosinémie héréditaire. Considérations sur l'ontogénése de la foetoprotéine humaine. Pathol Biol (Paris) 1973 May;21(5):457–463. [PubMed]
  • Waldmann TA, McIntire KR. Serum-alpha-fetoprotein levels in patients with ataxia-telangiectasia. Lancet. 1972 Nov 25;2(7787):1112–1115. [PubMed]
  • Kan YW, Dozy AM. Polymorphism of DNA sequence adjacent to human beta-globin structural gene: relationship to sickle mutation. Proc Natl Acad Sci U S A. 1978 Nov;75(11):5631–5635. [PMC free article] [PubMed]
  • Maniatis T, Jeffrey A, Kleid DG. Nucleotide sequence of the rightward operator of phage lambda. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184–1188. [PMC free article] [PubMed]
  • Lai EC, Woo SL, Dugaiczyk A, O'Malley BW. The ovalbumin gene: alleles created by mutations in the intervening sequences of the natural gene. Cell. 1979 Jan;16(1):201–211. [PubMed]
  • Harper ME, Saunders GF. Localization of single copy DNA sequences of G-banded human chromosomes by in situ hybridization. Chromosoma. 1981;83(3):431–439. [PubMed]
  • Chandler ME, Yunis JJ. A high resolution in situ hybridization technique for the direct visualization of labeled G-banded early metaphase and prophase chromosomes. Cytogenet Cell Genet. 1978;22(1-6):352–356. [PubMed]
  • Yunis JJ, Sawyer JR, Ball DW. Characterization of banding patterns of metaphase-prophase G-banded chromosomes and their use in gene mapping. Cytogenet Cell Genet. 1978;22(1-6):679–683. [PubMed]
  • Wahl GM, Stern M, Stark GR. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683–3687. [PMC free article] [PubMed]
  • Harper ME, Ullrich A, Saunders GF. Localization of the human insulin gene to the distal end of the short arm of chromosome 11. Proc Natl Acad Sci U S A. 1981 Jul;78(7):4458–4460. [PMC free article] [PubMed]
  • Harper ME, Barrera-Saldaña HA, Saunders GF. Chromosomal localization of the human placental lactogen-growth hormone gene cluster to 17q22-24. Am J Hum Genet. 1982 Mar;34(2):227–234. [PMC free article] [PubMed]
  • Weitkamp LR, Renwick JH, Berger J, Shreffler DC, Drachmann O, Wuhrmann F, Braend M, Franglen G. Additional data and summary for albumin-Ge linkage in man. Hum Hered. 1970;20(1):1–7. [PubMed]
  • Mikkelsen M, Jacobsen P, Henningsen K. Possible localization of Gc-System on chromosome 4. Loss of long arm 4 material associated with father-child incompatibility within the Gc-System. Hum Hered. 1977;27(2):105–107. [PubMed]
  • D'Eustachio P, Ingram RS, Tilghman SM, Ruddle FH. Murine alpha-fetoprotein and albumin: two evolutionarily linked proteins encoded on the same mouse chromosome. Somatic Cell Genet. 1981 May;7(3):289–294. [PubMed]
  • Ingram RS, Scott RW, Tilghman SM. alpha-Fetoprotein and albumin genes are in tandem in the mouse genome. Proc Natl Acad Sci U S A. 1981 Aug;78(8):4694–4698. [PMC free article] [PubMed]
  • Botstein D, White RL, Skolnick M, Davis RW. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet. 1980 May;32(3):314–331. [PMC free article] [PubMed]

Articles from American Journal of Human Genetics are provided here courtesy of American Society of Human Genetics

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...