Logo of aemPermissionsJournals.ASM.orgJournalAEM ArticleJournal InfoAuthorsReviewers
Appl Environ Microbiol. 1997 Jul; 63(7): 2516–2523.
PMCID: PMC168549

Ingredient selection for plastic composite supports for L-(+)-lactic acid biofilm fermentation by Lactobacillus casei subsp. rhamnosus.


Plastic composite supports containing 50% agricultural products (oat hulls, soybean hulls, yeast extract, soybean flour, dried bovine erythrocytes, bovine albumin, and/or mineral salts) and 50% (wt/wt) polypropylene were produced by high-temperature twin-screw extrusion. The research employed two half sets of a five-factorial fractional design (2(5 - 1)) to evaluate the effects of different agricultural components on the properties of the plastic composite supports and to select the best plastic composite support formulation for lactic acid fermentation. The biofilm population was affected by the contact angle and relative hydrophobicity of the supports (r = 0.79 to 0.82). Lactic acid was produced by the suspended cells (r = 0.96) and the biofilm on the plastic composite support discs (r = 0.85). Incorporation of yeast extract into plastic composite supports enhanced growth of free and attached cells in minimal medium (P < 0.0001). The presence of soybean hulls, yeast extract, or mineral salts in plastic composite supports produced less hydrophobic supports (P < 0.0001) and enhanced cell attachment (P < 0.03). Under all conditions, suspended-cell and polypropylene disc controls gave negligible lactic acid production and cell density. Plastic composite supports containing soybean hulls, yeast extract, soybean flour, bovine albumin, and mineral salts gave the highest biofilm population (2.3 x 10(9) CFU/g of support), cell density (absorbance of 1.8 at 620 nm), and lactic acid concentration (7.6 g/liter) in minimal medium.

Full Text

The Full Text of this article is available as a PDF (428K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Busscher HJ, Weerkamp AH, van der Mei HC, van Pelt AW, de Jong HP, Arends J. Measurement of the surface free energy of bacterial cell surfaces and its relevance for adhesion. Appl Environ Microbiol. 1984 Nov;48(5):980–983. [PMC free article] [PubMed]
  • Demirci A, Pometto AL, Johnson KE. Lactic Acid production in a mixed-culture biofilm reactor. Appl Environ Microbiol. 1993 Jan;59(1):203–207. [PMC free article] [PubMed]
  • Dickson JS, Koohmaraie M. Cell surface charge characteristics and their relationship to bacterial attachment to meat surfaces. Appl Environ Microbiol. 1989 Apr;55(4):832–836. [PMC free article] [PubMed]
  • Massol-Deyá AA, Whallon J, Hickey RF, Tiedje JM. Channel structures in aerobic biofilms of fixed-film reactors treating contaminated groundwater. Appl Environ Microbiol. 1995 Feb;61(2):769–777. [PMC free article] [PubMed]
  • van Loosdrecht MC, Lyklema J, Norde W, Schraa G, Zehnder AJ. The role of bacterial cell wall hydrophobicity in adhesion. Appl Environ Microbiol. 1987 Aug;53(8):1893–1897. [PMC free article] [PubMed]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


  • Compound
    PubChem Compound links
  • MedGen
    Related information in MedGen
  • PubMed
    PubMed citations for these articles
  • Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...