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Appl Environ Microbiol. Apr 1997; 63(4): 1434–1440.
PMCID: PMC168438

Application and evaluation of the phage resistance- and bacteriocin-encoding plasmid pMRC01 for the improvement of dairy starter cultures.

Abstract

The conjugative 63-kb lactococcal plasmid pMRC01 encodes bacteriophage resistance and production of and immunity to a novel broad-spectrum bacteriocin, designated lacticin 3147 (M.P. Ryan, M.C. Rea, C. Hill, and R.P. Ross, Appl. Environ. Microbiol. 62:612-619, 1996). The phage resistance is an abortive infection mechanism which targets the phage-lytic cycle at a point after phage DNA replication. By using the genetic determinants for bacteriocin immunity encoded on the plasmid as a selectable marker, pMRC01 was transferred into a variety of lactococcal starter cultures to improve their phage resistance properties. Selection of resulting transconjugants was performed directly on solid media containing the bacteriocin. Since the starters exhibited no spontaneous resistance to the bacteriocin as a selective agent, this allowed the assessment of the transfer of the naturally occurring plasmid into a range of dairy starter cultures. Results demonstrate that efficient transfer of the plasmid was dependent on the particular recipient strain chosen, and while high-frequency transfer (10(-3) per donor) of the entire plasmid to some strains was observed, the plasmid could not be conjugated into a number of starters. In this study, transconjugants for a number of lactococcal starter cultures which are phage resistant and bacteriocin producing have been generated. This bacteriocin-producing phenotype allows for control of nonstarter flora in food fermentations, and the phage resistance property protects the starter cultures in industry. The 63-kb plasmid was also successfully transferred into Lactococcus lactis MG1614 cells via electroporation.

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Selected References

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  • Anderson DG, McKay LL. Simple and rapid method for isolating large plasmid DNA from lactic streptococci. Appl Environ Microbiol. 1983 Sep;46(3):549–552. [PMC free article] [PubMed]
  • Behnke D, Malke H. Bacteriophage interference in Streptococcus pyogenes. I. Characterization of prophage--host systems interfering with the virulent phage A25. Virology. 1978 Mar;85(1):118–128. [PubMed]
  • Froseth BR, McKay LL. Molecular characterization of the nisin resistance region of Lactococcus lactis subsp. lactis biovar diacetylactis DRC3. Appl Environ Microbiol. 1991 Mar;57(3):804–811. [PMC free article] [PubMed]
  • Garvey P, Fitzgerald GF, Hill C. Cloning and DNA sequence analysis of two abortive infection phage resistance determinants from the lactococcal plasmid pNP40. Appl Environ Microbiol. 1995 Dec;61(12):4321–4328. [PMC free article] [PubMed]
  • Gasson MJ. Plasmid complements of Streptococcus lactis NCDO 712 and other lactic streptococci after protoplast-induced curing. J Bacteriol. 1983 Apr;154(1):1–9. [PMC free article] [PubMed]
  • Gireesh T, Davidson BE, Hillier AJ. Conjugal transfer in Lactococcus lactis of a 68-kilobase-pair chromosomal fragment containing the structural gene for the peptide bacteriocin nisin. Appl Environ Microbiol. 1992 May;58(5):1670–1676. [PMC free article] [PubMed]
  • Harrington A, Hill C. Construction of a Bacteriophage-Resistant Derivative of Lactococcus lactis subsp. lactis 425A by Using the Conjugal Plasmid pNP40. Appl Environ Microbiol. 1991 Dec;57(12):3405–3409. [PMC free article] [PubMed]
  • Hill C, Massey IJ, Klaenhammer TR. Rapid method to characterize lactococcal bacteriophage genomes. Appl Environ Microbiol. 1991 Jan;57(1):283–288. [PMC free article] [PubMed]
  • Jarvis AW, Heap HA, Limsowtin GK. Resistance against Industrial Bacteriophages Conferred on Lactococci by Plasmid pAJ1106 and Related Plasmids. Appl Environ Microbiol. 1989 Jun;55(6):1537–1543. [PMC free article] [PubMed]
  • Catherino WH, Jordan VC. Increasing the number of tandem estrogen response elements increases the estrogenic activity of a tamoxifen analogue. Cancer Lett. 1995 May 25;92(1):39–47. [PubMed]
  • Klaenhammer TR. Genetics of bacteriocins produced by lactic acid bacteria. FEMS Microbiol Rev. 1993 Sep;12(1-3):39–85. [PubMed]
  • Klaenhammer TR, Sanozky RB. Conjugal transfer from Streptococcus lactis ME2 of plasmids encoding phage resistance, nisin resistance and lactose-fermenting ability: evidence for a high-frequency conjugative plasmid responsible for abortive infection of virulent bacteriophage. J Gen Microbiol. 1985 Jun;131(6):1531–1541. [PubMed]
  • McKay LL, Baldwin KA. Conjugative 40-megadalton plasmid in Streptococcus lactis subsp. diacetylactis DRC3 is associated with resistance to nisin and bacteriophage. Appl Environ Microbiol. 1984 Jan;47(1):68–74. [PMC free article] [PubMed]
  • McKay LL, Baldwin KA, Zottola EA. Loss of lactose metabolism in lactic streptococci. Appl Microbiol. 1972 Jun;23(6):1090–1096. [PMC free article] [PubMed]
  • Platteeuw C, van Alen-Boerrigter I, van Schalkwijk S, de Vos WM. Food-grade cloning and expression system for Lactococcus lactis. Appl Environ Microbiol. 1996 Mar;62(3):1008–1013. [PMC free article] [PubMed]
  • Powell IB, Ward AC, Hillier AJ, Davidson BE. Simultaneous conjugal transfer in Lactococcus to genes involved in bacteriocin production and reduced susceptibility to bacteriophages. FEMS Microbiol Lett. 1990 Oct;60(1-2):209–213. [PubMed]
  • Ryan MP, Rea MC, Hill C, Ross RP. An application in cheddar cheese manufacture for a strain of Lactococcus lactis producing a novel broad-spectrum bacteriocin, lacticin 3147. Appl Environ Microbiol. 1996 Feb;62(2):612–619. [PMC free article] [PubMed]
  • Sanders ME, Klaenhammer TR. Restriction and modification in group N streptococci: effect of heat on development of modified lytic bacteriophage. Appl Environ Microbiol. 1980 Sep;40(3):500–506. [PMC free article] [PubMed]
  • Sanders ME, Leonhard PJ, Sing WD, Klaenhammer TR. Conjugal strategy for construction of fast Acid-producing, bacteriophage-resistant lactic streptococci for use in dairy fermentations. Appl Environ Microbiol. 1986 Nov;52(5):1001–1007. [PMC free article] [PubMed]
  • Steenson LR, Klaenhammer TR. Streptococcus cremoris M12R transconjugants carrying the conjugal plasmid pTR2030 are insensitive to attack by lytic bacteriophages. Appl Environ Microbiol. 1985 Oct;50(4):851–858. [PMC free article] [PubMed]
  • Terzaghi BE, Sandine WE. Improved medium for lactic streptococci and their bacteriophages. Appl Microbiol. 1975 Jun;29(6):807–813. [PMC free article] [PubMed]
  • Wells JM, Wilson PW, Le Page RW. Improved cloning vectors and transformation procedure for Lactococcus lactis. J Appl Bacteriol. 1993 Jun;74(6):629–636. [PubMed]

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