• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of aemPermissionsJournals.ASM.orgJournalAEM ArticleJournal InfoAuthorsReviewers
Appl Environ Microbiol. Feb 1997; 63(2): 796–799.
PMCID: PMC168372

Cellobiose dehydrogenase from Phanerochaete chrysosporium is encoded by two allelic variants.


The complete nucleotide sequences of two alleles of cellobiose dehydrogenase, cdh-1 (3,627 bp) and cdh-2 (3,623 bp), from Phanerochaete chrysosporium OGC101 are reported. The nucleotide sequences of cdh-1 and cdh-2 exhibit 97% similarity. A total of eighty-six point mutations between cdh-1 and cdh-2 are observed. Both alleles have 14 exons, and the introns are located at exactly the same positions. The translation products of these alleles have identical amino acid sequences. Restriction fragment length polymorphism analyses of homokaryotic derivatives show segregation of the CDH alleles.

Full Text

The Full Text of this article is available as a PDF (401K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Alic M, Letzring C, Gold MH. Mating System and Basidiospore Formation in the Lignin-Degrading Basidiomycete Phanerochaete chrysosporium. Appl Environ Microbiol. 1987 Jul;53(7):1464–1469. [PMC free article] [PubMed]
  • Ayers AR, Ayers SB, Eriksson KE. Cellobiose oxidase, purification and partial characterization of a hemoprotein from Sporotrichum pulverulentum. Eur J Biochem. 1978 Sep 15;90(1):171–181. [PubMed]
  • Bao W, Renganathan V. Cellobiose oxidase of Phanerochaete chrysosporium enhances crystalline cellulose degradation by cellulases. FEBS Lett. 1992 May 4;302(1):77–80. [PubMed]
  • Bao W, Usha SN, Renganathan V. Purification and characterization of cellobiose dehydrogenase, a novel extracellular hemoflavoenzyme from the white-rot fungus Phanerochaete chrysosporium. Arch Biochem Biophys. 1993 Feb 1;300(2):705–713. [PubMed]
  • Breathnach R, Benoist C, O'Hare K, Gannon F, Chambon P. Ovalbumin gene: evidence for a leader sequence in mRNA and DNA sequences at the exon-intron boundaries. Proc Natl Acad Sci U S A. 1978 Oct;75(10):4853–4857. [PMC free article] [PubMed]
  • Canevascini G, Borer P, Dreyer JL. Cellobiose dehydrogenases of Sporotrichum (Chrysosporium) thermophile. Eur J Biochem. 1991 May 23;198(1):43–52. [PubMed]
  • Covert SF, Bolduc J, Cullen D. Genomic organization of a cellulase gene family in Phanerochaete chrysosporium. Curr Genet. 1992 Nov;22(5):407–413. [PubMed]
  • Covert SF, Vanden Wymelenberg A, Cullen D. Structure, organization, and transcription of a cellobiohydrolase gene cluster from Phanerochaete chrysosporium. Appl Environ Microbiol. 1992 Jul;58(7):2168–2175. [PMC free article] [PubMed]
  • Gaskell J, Dieperink E, Cullen D. Genomic organization of lignin peroxidase genes of Phanerochaete chrysosporium. Nucleic Acids Res. 1991 Feb 11;19(3):599–603. [PMC free article] [PubMed]
  • Gold MH, Alic M. Molecular biology of the lignin-degrading basidiomycete Phanerochaete chrysosporium. Microbiol Rev. 1993 Sep;57(3):605–622. [PMC free article] [PubMed]
  • Henriksson G, Pettersson G, Johansson G, Ruiz A, Uzcategui E. Cellobiose oxidase from Phanerochaete chrysosporium can be cleaved by papain into two domains. Eur J Biochem. 1991 Feb 26;196(1):101–106. [PubMed]
  • Kersten PJ, Witek C, vanden Wymelenberg A, Cullen D. Phanerochaete chrysosporium glyoxal oxidase is encoded by two allelic variants: structure, genomic organization, and heterologous expression of glx1 and glx2. J Bacteriol. 1995 Nov;177(21):6106–6110. [PMC free article] [PubMed]
  • Li B, Nagalla SR, Renganathan V. Cloning of a cDNA encoding cellobiose dehydrogenase, a hemoflavoenzyme from Phanerochaete chrysosporium. Appl Environ Microbiol. 1996 Apr;62(4):1329–1335. [PMC free article] [PubMed]
  • Lymar ES, Li B, Renganathan V. Purification and Characterization of a Cellulose-Binding (beta)-Glucosidase from Cellulose-Degrading Cultures of Phanerochaete chrysosporium. Appl Environ Microbiol. 1995 Aug;61(8):2976–2980. [PMC free article] [PubMed]
  • Raices M, Paifer E, Cremata J, Montesino R, Ståhlberg J, Divne C, Szabó IJ, Henriksson G, Johansson G, Pettersson G. Cloning and characterization of a cDNA encoding a cellobiose dehydrogenase from the white rot fungus Phanerochaete chrysosporium. FEBS Lett. 1995 Aug 7;369(2-3):233–238. [PubMed]
  • Roy BP, Archibald F. An indirect free radical-based assay for the enzyme cellobiose:quinone oxidoreductase. Anal Biochem. 1994 Feb 1;216(2):291–298. [PubMed]
  • Schmidhalter DR, Canevascini G. Isolation and characterization of the cellobiose dehydrogenase from the brown-rot fungus Coniophora puteana (Schum ex Fr.) Karst. Arch Biochem Biophys. 1993 Feb 1;300(2):559–563. [PubMed]
  • Sims PF, Soares-Felipe MS, Wang Q, Gent ME, Tempelaars C, Broda P. Differential expression of multiple exo-cellobiohydrolase I-like genes in the lignin-degrading fungus Phanerochaete chrysosporium. Mol Microbiol. 1994 Apr;12(2):209–216. [PubMed]
  • Strauss EC, Kobori JA, Siu G, Hood LE. Specific-primer-directed DNA sequencing. Anal Biochem. 1986 Apr;154(1):353–360. [PubMed]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...