• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of ajhgLink to Publisher's site
Am J Hum Genet. Apr 1991; 48(4): 783–790.
PMCID: PMC1682947

A t(4;22) in a meningioma points to the localization of a putative tumor-suppressor gene.

Abstract

Cytogenetic analysis of meningioma cells from one particular patient (MN32) displayed the stem-line karyo-type 45, XY, -1, 4p+, 22q-, 22q+, which thus had rearrangements of both chromosomes 22. The 22q+ marker appeared as a dicentric: 22 pter----q11::1p11----qter. The reciprocal product of this translocation has presumably been lost because it lacked a centromere. The 22q- chromosome also appeared to have lost sequences distal to band q11. We assumed that this marker could have been the result of a reciprocal translocation between chromosomes 4 and 22. To investigate the 4p+ and 22q- chromosomes in more detail, human-hamster somatic cell hybrids were constructed that segregated the 22q- and 4p+ chromosomes. Southern blot analysis with DNA from these hybrids showed that sequences from 22q were indeed translocated to 4p+ and that reciprocally sequences from 4p were translocated to 22q-, demonstrating a balanced t(4;22)(p16;q11). On the basis of these results we presume that in this tumor a tumor-suppressor gene is deleted in the case of the 22q+ marker and that the t(4;22) disrupts the second allele of this gene. The latter translocation was mapped between D22S1 and D22S15, a distance of 1 cM on the linkage map of this chromosome. The area in which we have located the translocation is within the region where the gene predisposing to neurofibromatosis 2 has been mapped.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (2.2M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Images in this article

Click on the image to see a larger version.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Al Saadi A, Latimer F, Madercic M, Robbins T. Cytogenetic studies of human brain tumors and their clinical significance. II. Meningioma. Cancer Genet Cytogenet. 1987 May;26(1):127–141. [PubMed]
  • Amasino RM. Acceleration of nucleic acid hybridization rate by polyethylene glycol. Anal Biochem. 1986 Feb 1;152(2):304–307. [PubMed]
  • Bakker E, Skraastad MI, Fisser-Groen YM, van Ommen GJ, Pearson PL. Two additional RFLPs at the D4S10 locus, useful for Huntington's disease (HD)-family studies. Nucleic Acids Res. 1987 Nov 11;15(21):9100–9100. [PMC free article] [PubMed]
  • Barker D, Schafer M, White R. Restriction sites containing CpG show a higher frequency of polymorphism in human DNA. Cell. 1984 Jan;36(1):131–138. [PubMed]
  • Bućan M, Zimmer M, Whaley WL, Poustka A, Youngman S, Allitto BA, Ormondroyd E, Smith B, Pohl TM, MacDonald M, et al. Physical maps of 4p16.3, the area expected to contain the Huntington disease mutation. Genomics. 1990 Jan;6(1):1–15. [PubMed]
  • Call KM, Glaser T, Ito CY, Buckler AJ, Pelletier J, Haber DA, Rose EA, Kral A, Yeger H, Lewis WH, et al. Isolation and characterization of a zinc finger polypeptide gene at the human chromosome 11 Wilms' tumor locus. Cell. 1990 Feb 9;60(3):509–520. [PubMed]
  • Casalone R, Granata P, Simi P, Tarantino E, Butti G, Buonaguidi R, Faggionato F, Knerich R, Solero L. Recessive cancer genes in meningiomas? An analysis of 31 cases. Cancer Genet Cytogenet. 1987 Jul;27(1):145–159. [PubMed]
  • Casartelli C, Rogatto SR, Barbieri Neto J. Karyotypic evolution of human meningioma. Progression through malignancy. Cancer Genet Cytogenet. 1989 Jul 1;40(1):33–45. [PubMed]
  • Cavenee WK, Koufos A, Hansen MF. Recessive mutant genes predisposing to human cancer. Mutat Res. 1986 Jul;168(1):3–14. [PubMed]
  • Cawthon RM, Weiss R, Xu GF, Viskochil D, Culver M, Stevens J, Robertson M, Dunn D, Gesteland R, O'Connell P, et al. A major segment of the neurofibromatosis type 1 gene: cDNA sequence, genomic structure, and point mutations. Cell. 1990 Jul 13;62(1):193–201. [PubMed]
  • Cheng SV, Martin GR, Nadeau JH, Haines JL, Bucan M, Kozak CA, MacDonald ME, Lockyer JL, Ledley FD, Woo SL, et al. Synteny on mouse chromosome 5 of homologs for human DNA loci linked to the Huntington disease gene. Genomics. 1989 Apr;4(3):419–426. [PubMed]
  • Dumanski JP, Carlbom E, Collins VP, Nordenskjöld M. Deletion mapping of a locus on human chromosome 22 involved in the oncogenesis of meningioma. Proc Natl Acad Sci U S A. 1987 Dec;84(24):9275–9279. [PMC free article] [PubMed]
  • Eliyahu D, Michalovitz D, Eliyahu S, Pinhasi-Kimhi O, Oren M. Wild-type p53 can inhibit oncogene-mediated focus formation. Proc Natl Acad Sci U S A. 1989 Nov;86(22):8763–8767. [PMC free article] [PubMed]
  • Fearon ER, Cho KR, Nigro JM, Kern SE, Simons JW, Ruppert JM, Hamilton SR, Preisinger AC, Thomas G, Kinzler KW, et al. Identification of a chromosome 18q gene that is altered in colorectal cancers. Science. 1990 Jan 5;247(4938):49–56. [PubMed]
  • Feinberg AP, Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. [PubMed]
  • Finlay CA, Hinds PW, Levine AJ. The p53 proto-oncogene can act as a suppressor of transformation. Cell. 1989 Jun 30;57(7):1083–1093. [PubMed]
  • Gessler M, Poustka A, Cavenee W, Neve RL, Orkin SH, Bruns GA. Homozygous deletion in Wilms tumours of a zinc-finger gene identified by chromosome jumping. Nature. 1990 Feb 22;343(6260):774–778. [PubMed]
  • Geurts van Kessel AH, ten Brinke H, Boere WA, den Boer WC, de Groot PG, Hagemeijer A, Meera Khan P, Pearson PL. Characterization of the Philadelphia chromosome by gene mapping. Cytogenet Cell Genet. 1981;30(2):83–91. [PubMed]
  • Heisterkamp N, Groffen J, Stephenson JR. The human v-abl cellular homologue. J Mol Appl Genet. 1983;2(1):57–68. [PubMed]
  • Heisterkamp N, Stam K, Groffen J, de Klein A, Grosveld G. Structural organization of the bcr gene and its role in the Ph' translocation. Nature. 315(6022):758–761. [PubMed]
  • Julier C, Lathrop GM, Reghis A, Szajnert MF, Lalouel JM, Kaplan JC. A linkage and physical map of chromosome 22, and some applications to gene mapping. Am J Hum Genet. 1988 Feb;42(2):297–308. [PMC free article] [PubMed]
  • Katsuyama J, Papenhausen PR, Herz F, Gazivoda P, Hirano A, Koss LG. Chromosome abnormalities in meningiomas. Cancer Genet Cytogenet. 1986 May;22(1):63–68. [PubMed]
  • Koper JW, Foekens JA, Braakman R, Lamberts SW. Effects of progesterone on the response to epidermal growth factor and other growth factors in cultured human meningioma cells. Cancer Res. 1990 May 1;50(9):2604–2607. [PubMed]
  • McKeon C, Thiele CJ, Ross RA, Kwan M, Triche TJ, Miser JS, Israel MA. Indistinguishable patterns of protooncogene expression in two distinct but closely related tumors: Ewing's sarcoma and neuroepithelioma. Cancer Res. 1988 Aug 1;48(15):4307–4311. [PubMed]
  • Maltby EL, Ironside JW, Battersby RD. Cytogenetic studies in 50 meningiomas. Cancer Genet Cytogenet. 1988 Apr;31(2):199–210. [PubMed]
  • Meese E, Blin N, Zang KD. Loss of heterozygosity and the origin of meningioma. Hum Genet. 1987 Dec;77(4):349–351. [PubMed]
  • Nakamura Y, Culver M, O'Connell P, Leppert M, Lathrop GM, Lalouel JM, White R. Isolation and mapping of a polymorphic DNA sequence (pYNZ32) on chromosome 4p [D4S125]. Nucleic Acids Res. 1988 May 11;16(9):4186–4186. [PMC free article] [PubMed]
  • Poulsgård L, Rønne M, Schrøder HD. Cytogenetic studies of 19 meningiomas and their clinical significance. I. Anticancer Res. 1989 Jan-Feb;9(1):109–112. [PubMed]
  • Preston-Martin S, Paganini-Hill A, Henderson BE, Pike MC, Wood C. Case-control study of intracranial meningiomas in women in Los Angeles County, California. J Natl Cancer Inst. 1980 Jul;65(1):67–73. [PubMed]
  • Preston-Martin S, Yu MC, Henderson BE, Roberts C. Risk factors for meningiomas in men in Los Angeles County. J Natl Cancer Inst. 1983 May;70(5):863–866. [PubMed]
  • Rey JA, Bello MJ, de Campos JM, Kusak E, Moreno S. Chromosomal involvement secondary to -22 in human meningiomas. Cancer Genet Cytogenet. 1988 Jul 15;33(2):275–290. [PubMed]
  • Rose EA, Glaser T, Jones C, Smith CL, Lewis WH, Call KM, Minden M, Champagne E, Bonetta L, Yeger H, et al. Complete physical map of the WAGR region of 11p13 localizes a candidate Wilms' tumor gene. Cell. 1990 Feb 9;60(3):495–508. [PubMed]
  • Rouleau GA, Haines JL, Bazanowski A, Colella-Crowley A, Trofatter JA, Wexler NS, Conneally PM, Gusella JF. A genetic linkage map of the long arm of human chromosome 22. Genomics. 1989 Jan;4(1):1–6. [PubMed]
  • Rouleau GA, Kurnit DM, Neve RL, Bazanowsky A, Patterson D, Gusella JF. D22S15--a fetal brain cDNA with BanII and SacI RFLP. Nucleic Acids Res. 1988 Feb 25;16(4):1646–1646. [PMC free article] [PubMed]
  • Rouleau GA, Seizinger BR, Wertelecki W, Haines JL, Superneau DW, Martuza RL, Gusella JF. Flanking markers bracket the neurofibromatosis type 2 (NF2) gene on chromosome 22. Am J Hum Genet. 1990 Feb;46(2):323–328. [PMC free article] [PubMed]
  • Seizinger BR, Martuza RL, Gusella JF. Loss of genes on chromosome 22 in tumorigenesis of human acoustic neuroma. Nature. 1986 Aug 14;322(6080):644–647. [PubMed]
  • Seizinger BR, Rouleau G, Ozelius LJ, Lane AH, St George-Hyslop P, Huson S, Gusella JF, Martuza RL. Common pathogenetic mechanism for three tumor types in bilateral acoustic neurofibromatosis. Science. 1987 Apr 17;236(4799):317–319. [PubMed]
  • Thayer RE, Harper ME, Sawyer J, Singer MF, McBride OW. Localization of a unique DNA sequence to band p16 of human chromosome 4. Cytogenet Cell Genet. 1987;45(2):75–79. [PubMed]
  • Turc-Carel C, Aurias A, Mugneret F, Lizard S, Sidaner I, Volk C, Thiery JP, Olschwang S, Philip I, Berger MP, et al. Chromosomes in Ewing's sarcoma. I. An evaluation of 85 cases of remarkable consistency of t(11;22)(q24;q12). Cancer Genet Cytogenet. 1988 Jun;32(2):229–238. [PubMed]
  • Viskochil D, Buchberg AM, Xu G, Cawthon RM, Stevens J, Wolff RK, Culver M, Carey JC, Copeland NG, Jenkins NA, et al. Deletions and a translocation interrupt a cloned gene at the neurofibromatosis type 1 locus. Cell. 1990 Jul 13;62(1):187–192. [PubMed]
  • Wallace MR, Marchuk DA, Andersen LB, Letcher R, Odeh HM, Saulino AM, Fountain JW, Brereton A, Nicholson J, Mitchell AL, et al. Type 1 neurofibromatosis gene: identification of a large transcript disrupted in three NF1 patients. Science. 1990 Jul 13;249(4965):181–186. [PubMed]
  • Weinberg RA. Oncogenes, antioncogenes, and the molecular bases of multistep carcinogenesis. Cancer Res. 1989 Jul 15;49(14):3713–3721. [PubMed]
  • Weller P, Jeffreys AJ, Wilson V, Blanchetot A. Organization of the human myoglobin gene. EMBO J. 1984 Feb;3(2):439–446. [PMC free article] [PubMed]
  • Wertelecki W, Rouleau GA, Superneau DW, Forehand LW, Williams JP, Haines JL, Gusella JF. Neurofibromatosis 2: clinical and DNA linkage studies of a large kindred. N Engl J Med. 1988 Aug 4;319(5):278–283. [PubMed]
  • Zang KD. Cytological and cytogenetical studies on human meningioma. Cancer Genet Cytogenet. 1982 Jul;6(3):249–274. [PubMed]
  • Zhang FR, Delattre O, Rouleau G, Couturier J, Lefrançois, Thomas G, Aurias A. The neuroepithelioma breakpoint on chromosome 22 is proximal to the meningioma locus. Genomics. 1990 Jan;6(1):174–177. [PubMed]

Articles from American Journal of Human Genetics are provided here courtesy of American Society of Human Genetics

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • Compound
    Compound
    PubChem Compound links
  • MedGen
    MedGen
    Related information in MedGen
  • OMIM
    OMIM
    OMIM record citing PubMed
  • PubMed
    PubMed
    PubMed citations for these articles
  • Substance
    Substance
    PubChem Substance links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...