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Appl Environ Microbiol. Dec 1996; 62(12): 4381–4387.
PMCID: PMC168265

Production of pediocin AcH by Lactobacillus plantarum WHE 92 isolated from cheese.


Among 1,962 bacterial isolates from a smear-surface soft cheese (Munster cheese) screened for activity against Listeria monocytogenes, six produced antilisterial compounds other than organic acids. The bacterial strain WHE 92, which displayed the strongest antilisterial effect, was identified at the DNA level as Lactobacillus plantarum. The proteinaceous nature, narrow inhibitory spectrum, and bactericidal mode of action of the antilisterial compound produced by this bacterium suggested that it was a bacteriocin. Purification to homogeneity and sequencing of this bacteriocin showed that it was a 4.6-kDa, 44-amino-acid peptide, the primary structure of which was identical to that of pediocin AcH produced by different Pediococcus acidilactici strains. We report the first case of the same bacteriocin appearing naturally with bacteria of different genera. Whereas the production of pediocin AcH from P. acidilactici H was considerably reduced when the final pH of the medium exceeded 5.0, no reduction in the production of pediocin AcH from L. plantarum WHE 92 was observed when the pH of the medium was up to 6.0. This fact is important from an industrial angle. As the pH of dairy products is often higher than 5.0, L. plantarum WHE 92, which develops particularly well in cheeses, could constitute an effective means of biological combat against L. monocytogenes in this type of foodstuff.

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Selected References

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  • Allison GE, Worobo RW, Stiles ME, Klaenhammer TR. Heterologous expression of the lactacin F peptides by Carnobacterium piscicola LV17. Appl Environ Microbiol. 1995 Apr;61(4):1371–1377. [PMC free article] [PubMed]
  • Barefoot SF, Klaenhammer TR. Detection and activity of lactacin B, a bacteriocin produced by Lactobacillus acidophilus. Appl Environ Microbiol. 1983 Jun;45(6):1808–1815. [PMC free article] [PubMed]
  • Biswas SR, Ray P, Johnson MC, Ray B. Influence of Growth Conditions on the Production of a Bacteriocin, Pediocin AcH, by Pediococcus acidilactici H. Appl Environ Microbiol. 1991 Apr;57(4):1265–1267. [PMC free article] [PubMed]
  • Bringel F, Curk MC, Hubert JC. Characterization of lactobacilli by Southern-type hybridization with a Lactobacillus plantarum pyrDFE probe. Int J Syst Bacteriol. 1996 Apr;46(2):588–594. [PubMed]
  • Broadbent JR, Kondo JK. Genetic construction of nisin-producing Lactococcus lactis subsp. cremoris and analysis of a rapid method for conjugation. Appl Environ Microbiol. 1991 Feb;57(2):517–524. [PMC free article] [PubMed]
  • Diep DB, Håvarstein LS, Nissen-Meyer J, Nes IF. The gene encoding plantaricin A, a bacteriocin from Lactobacillus plantarum C11, is located on the same transcription unit as an agr-like regulatory system. Appl Environ Microbiol. 1994 Jan;60(1):160–166. [PMC free article] [PubMed]
  • Farber JM, Peterkin PI. Listeria monocytogenes, a food-borne pathogen. Microbiol Rev. 1991 Sep;55(3):476–511. [PMC free article] [PubMed]
  • Garver KI, Muriana PM. Detection, identification and characterization of bacteriocin-producing lactic acid bacteria from retail food products. Int J Food Microbiol. 1993 Sep;19(4):241–258. [PubMed]
  • Gibson EM, Chace NM, London SB, London J. Transfer of plasmid-mediated antibiotic resistance from streptococci to lactobacilli. J Bacteriol. 1979 Jan;137(1):614–619. [PMC free article] [PubMed]
  • Gireesh T, Davidson BE, Hillier AJ. Conjugal transfer in Lactococcus lactis of a 68-kilobase-pair chromosomal fragment containing the structural gene for the peptide bacteriocin nisin. Appl Environ Microbiol. 1992 May;58(5):1670–1676. [PMC free article] [PubMed]
  • González B, Arca P, Mayo B, Suárez JE. Detection, purification, and partial characterization of plantaricin C, a bacteriocin produced by a Lactobacillus plantarum strain of dairy origin. Appl Environ Microbiol. 1994 Jun;60(6):2158–2163. [PMC free article] [PubMed]
  • Gonzalez CF, Kunka BS. Plasmid transfer in Pediococcus spp.: intergeneric and intrageneric transfer of pIP501. Appl Environ Microbiol. 1983 Jul;46(1):81–89. [PMC free article] [PubMed]
  • Gonzalez CF, Kunka BS. Transfer of Sucrose-Fermenting Ability and Nisin Production Phenotype among Lactic Streptococci. Appl Environ Microbiol. 1985 Mar;49(3):627–633. [PMC free article] [PubMed]
  • Henderson JT, Chopko AL, van Wassenaar PD. Purification and primary structure of pediocin PA-1 produced by Pediococcus acidilactici PAC-1.0. Arch Biochem Biophys. 1992 May 15;295(1):5–12. [PubMed]
  • Jeppesen VF, Huss HH. Characteristics and antagonistic activity of lactic acid bacteria isolated from chilled fish products. Int J Food Microbiol. 1993 Jun 1;18(4):305–320. [PubMed]
  • Jiménez-Díaz R, Ruiz-Barba JL, Cathcart DP, Holo H, Nes IF, Sletten KH, Warner PJ. Purification and partial amino acid sequence of plantaricin S, a bacteriocin produced by Lactobacillus plantarum LPCO10, the activity of which depends on the complementary action of two peptides. Appl Environ Microbiol. 1995 Dec;61(12):4459–4463. [PMC free article] [PubMed]
  • Klaenhammer TR. Bacteriocins of lactic acid bacteria. Biochimie. 1988 Mar;70(3):337–349. [PubMed]
  • Klaenhammer TR. Genetics of bacteriocins produced by lactic acid bacteria. FEMS Microbiol Rev. 1993 Sep;12(1-3):39–85. [PubMed]
  • Lepage P, Bitsch F, Roecklin D, Keppi E, Dimarcq JL, Reichhart JM, Hoffmann JA, Roitsch C, Van Dorseelaer A. Determination of disulfide bridges in natural and recombinant insect defensin A. Eur J Biochem. 1991 Mar 28;196(3):735–742. [PubMed]
  • Lewus CB, Kaiser A, Montville TJ. Inhibition of food-borne bacterial pathogens by bacteriocins from lactic acid bacteria isolated from meat. Appl Environ Microbiol. 1991 Jun;57(6):1683–1688. [PMC free article] [PubMed]
  • Luchansky JB, Glass KA, Harsono KD, Degnan AJ, Faith NG, Cauvin B, Baccus-Taylor G, Arihara K, Bater B, Maurer AJ, et al. Genomic analysis of Pediococcus starter cultures used to control Listeria monocytogenes in turkey summer sausage. Appl Environ Microbiol. 1992 Sep;58(9):3053–3059. [PMC free article] [PubMed]
  • Marugg JD, Gonzalez CF, Kunka BS, Ledeboer AM, Pucci MJ, Toonen MY, Walker SA, Zoetmulder LC, Vandenbergh PA. Cloning, expression, and nucleotide sequence of genes involved in production of pediocin PA-1, and bacteriocin from Pediococcus acidilactici PAC1.0. Appl Environ Microbiol. 1992 Aug;58(8):2360–2367. [PMC free article] [PubMed]
  • Motlagh AM, Bhunia AK, Szostek F, Hansen TR, Johnson MC, Ray B. Nucleotide and amino acid sequence of pap-gene (pediocin AcH production) in Pediococcus acidilactici H. Lett Appl Microbiol. 1992 Aug;15(2):45–48. [PubMed]
  • Motlagh A, Bukhtiyarova M, Ray B. Complete nucleotide sequence of pSMB 74, a plasmid encoding the production of pediocin AcH in Pediococcus acidilactici. Lett Appl Microbiol. 1994 Jun;18(6):305–312. [PubMed]
  • Mundt JO, Beattie WG, Wieland FR. Pediococci residing on plants. J Bacteriol. 1969 Jun;98(3):938–942. [PMC free article] [PubMed]
  • Muriana PM, Klaenhammer TR. Conjugal Transfer of Plasmid-Encoded Determinants for Bacteriocin Production and Immunity in Lactobacillus acidophilus 88. Appl Environ Microbiol. 1987 Mar;53(3):553–560. [PMC free article] [PubMed]
  • Neve H, Geis A, Teuber M. Conjugal transfer and characterization of bacteriocin plasmids in group N (lactic acid) streptococci. J Bacteriol. 1984 Mar;157(3):833–838. [PMC free article] [PubMed]
  • Nissen-Meyer J, Holo H, Håvarstein LS, Sletten K, Nes IF. A novel lactococcal bacteriocin whose activity depends on the complementary action of two peptides. J Bacteriol. 1992 Sep;174(17):5686–5692. [PMC free article] [PubMed]
  • Nissen-Meyer J, Larsen AG, Sletten K, Daeschel M, Nes IF. Purification and characterization of plantaricin A, a Lactobacillus plantarum bacteriocin whose activity depends on the action of two peptides. J Gen Microbiol. 1993 Sep;139(9):1973–1978. [PubMed]
  • Sung MW, Johnson JT, Van Dongen G, Whiteside TL. Protective effects of interferon-gamma on squamous-cell carcinoma of head and neck targets in antibody-dependent cellular cytotoxicity mediated by human natural killer cells. Int J Cancer. 1996 May 3;66(3):393–399. [PubMed]
  • Schved F, Lalazar A, Henis Y, Juven BJ. Purification, partial characterization and plasmid-linkage of pediocin SJ-1, a bacteriocin produced by Pediococcus acidilactici. J Appl Bacteriol. 1993 Jan;74(1):67–77. [PubMed]
  • Steele JL, McKay LL. Partial characterization of the genetic basis for sucrose metabolism and nisin production in Streptococcus lactis. Appl Environ Microbiol. 1986 Jan;51(1):57–64. [PMC free article] [PubMed]
  • Stoddard GW, Petzel JP, van Belkum MJ, Kok J, McKay LL. Molecular analyses of the lactococcin A gene cluster from Lactococcus lactis subsp. lactis biovar diacetylactis WM4. Appl Environ Microbiol. 1992 Jun;58(6):1952–1961. [PMC free article] [PubMed]
  • Tagg JR, McGiven AR. Assay system for bacteriocins. Appl Microbiol. 1971 May;21(5):943–943. [PMC free article] [PubMed]
  • Terzaghi BE, Sandine WE. Improved medium for lactic streptococci and their bacteriophages. Appl Microbiol. 1975 Jun;29(6):807–813. [PMC free article] [PubMed]
  • Tsai HJ, Sandine WE. Conjugal transfer of nisin plasmid genes from Streptococcus lactis 7962 to Leuconostoc dextranicum 181. Appl Environ Microbiol. 1987 Feb;53(2):352–357. [PMC free article] [PubMed]
  • van Belkum MJ, Kok J, Venema G, Holo H, Nes IF, Konings WN, Abee T. The bacteriocin lactococcin A specifically increases permeability of lactococcal cytoplasmic membranes in a voltage-independent, protein-mediated manner. J Bacteriol. 1991 Dec;173(24):7934–7941. [PMC free article] [PubMed]
  • van Belkum MJ, Stiles ME. Molecular characterization of genes involved in the production of the bacteriocin leucocin A from Leuconostoc gelidum. Appl Environ Microbiol. 1995 Oct;61(10):3573–3579. [PMC free article] [PubMed]
  • Vanderslice P, Copeland WC, Robertus JD. Cloning and nucleotide sequence of wild type and a mutant histidine decarboxylase from Lactobacillus 30a. J Biol Chem. 1986 Nov 15;261(32):15186–15191. [PubMed]
  • Vescovo M, Morelli L, Bottazzi V, Gasson MJ. Conjugal Transfer of Broad-Host-Range Plasmid pAMbeta1 into Enteric Species of Lactic Acid Bacteria. Appl Environ Microbiol. 1983 Sep;46(3):753–755. [PMC free article] [PubMed]
  • West CA, Warner PJ. Plasmid profiles and transfer of plasmid-encoded antibiotic resistance in Lactobacillus plantarum. Appl Environ Microbiol. 1985 Nov;50(5):1319–1321. [PMC free article] [PubMed]

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