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Br Med J. May 24, 1975; 2(5968): 409–411.
PMCID: PMC1681821

Detection of hepatitis B surface antigen among Scottish blood donors: evaluation of sensitive tanned-cell haemagglutination-inhibition test.


A total of 70 224 blood donations were tested at three Scottish blood transfusion centres for hepatitis B surface antigen /HBsAg) by an economical haemagglutination-inhibition method (E.H.A.I.) and the results compared with those of counterelectrophoresis (C.E.P.). A further 4086 donations were tested using the Wellcome turkey cell haemagglutination test, C.E.P., and E.H.A.I.E.H.A.I. was also compared with commercial haemagglutination and radioimmunoassay reagents for sensitivity and specificity against several established antigen panels and used to reinvestigate counterelectrophoresis-negative blood donations implicated in post-transfusion hepatitis. E.H.A.I. combines the inherent specificity of an inhibition reaction with a sensitivity equal to that of commercial radioimmunoassay and haemagglutination kits but at a fraction of the cost. The assessment of 70 224 blood donations in three regions showed that E.H.A.I. detected more antigen-positive blood donations than C.E.P. Results of retesting more than 100 blood donors implicated in 10 cases of post-transfusion hepatitis suggested that the use of E.H.A.I. or a test of similar sensitivity in place of C.E.P. may significantly reduce the incidence of this complication.

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Selected References

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  • Giles JP, McCollum RW, Berndtson LW, Jr, Krugman S. Relation of Australia-SH antigen to the willowbrook MS-2 strain. N Engl J Med. 1969 Jul 17;281(3):119–122. [PubMed]
  • Hoofnagle JH, Gerety RJ, Ni LY, Barker LF. Antibody to hepatitis B core antigen. A sensitive indicator of hepatitis B virus replication. N Engl J Med. 1974 Jun 13;290(24):1336–1340. [PubMed]
  • Prince AM, Brotman B, Grady GF, Kuhns WJ, Hazzi C, Levine RW, Millian SJ. Long-incubation post-transfusion hepatitis without serological evidence of exposure to hepatitis-B virus. Lancet. 1974 Aug 3;2(7875):241–246. [PubMed]
  • Hopkins R, Das PC. Improved sensitivity of the electrophoresis method by tannic acid for detection of Australia antigen. J Clin Pathol. 1972 Sep;25(9):832–833. [PMC free article] [PubMed]
  • Ling CM, Overby LR. Prevalence of hepatitis B virus antigen as revealed by direct radioimmune assay with 125 I-antibody. J Immunol. 1972 Oct;109(4):834–841. [PubMed]
  • Germain KH, Sturdivant SK, Rightsel WA. Evaluation of a red cell agglutination test for detection of Australia antigen (HG Ag). Appl Microbiol. 1973 Apr;25(4):524–527. [PMC free article] [PubMed]
  • Reesink HW, Duimel WJ, Brummelhuis HG. Evaluation of a new haemagglutination technique for the demonstration of hepatitis-B antigen. Lancet. 1973 Dec 15;2(7842):1351–1353. [PubMed]
  • Cayzer I, Dane DS, Cameron CH, Denning JV. A rapid haemagglutination test for hepatitis-B antigen. Lancet. 1974 May 18;1(7864):947–949. [PubMed]
  • Hopkins R, Das PC. Australia antigen (HB-Ag) subtyping by a sensitive tanned cell haemagglutination-inhibition technique. Br J Haematol. 1974 Jul;27(3):501–506. [PubMed]
  • Vandervelde EM, Mahmood N, Goffin C, Porter A, Megson B, Cossart YE. User's guide to some new tests for hepatitis-B antigen. Lancet. 1974 Nov 2;2(7888):1066–1068. [PubMed]
  • Chrystie IL, Islam MN, Banatvala JE. Clinical evaluation of the turkey-erythrocyte passive-haemagglutination test for hepatitis-B surface antigen. Lancet. 1974 Jun 15;1(7868):1193–1194. [PubMed]
  • Koretz RL, Klahs DR, Ritman S, Damus KH, Gitnick GL. Post-transfusion hepatitis in recipients of blood screened by newer assays. Lancet. 1973 Sep 29;2(7831):694–696. [PubMed]

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