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Appl Environ Microbiol. Sep 1996; 62(9): 3459–3461.
PMCID: PMC168143

Aeromonas hydrophila isolated from food and drinking water: hemagglutination, hemolysis, and cytotoxicity for a human intestinal cell line (HT-29).


Aeromonas hydrophila isolated from food and drinking water was tested for pathogenicity by studying its hemolysis, hemagglutination, and cytotoxicity. Hemolysis, tested on erythrocytes from six different species, was more frequently seen with water isolates (64%) than with food isolates (48%). Hemagglutination was more frequently encountered with food isolates (92%) than with water isolates (73%). Cytotoxicity, evaluated on seven cell lines, was frequently observed with food isolates (92%) and with water isolates (73%). Heat treatment (56 degrees C for 10 min) of culture supernatant fluids inhibited the toxicity of some but not all toxin-producing isolates. Our results suggest that the human intestinal cell line HT-29 could be a useful complement for testing A. hydrophila exotoxins and for studying the enteropathogenicity of this species for humans.

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Selected References

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  • Atkinson HM, Trust TJ. Hemagglutination properties and adherence ability of Aeromonas hydrophila. Infect Immun. 1980 Mar;27(3):938–946. [PMC free article] [PubMed]
  • Bernheimer AW, Avigad LS, Avigad G. Interactions between aerolysin, erythrocytes, and erythrocyte membranes. Infect Immun. 1975 Jun;11(6):1312–1319. [PMC free article] [PubMed]
  • Burke V, Cooper M, Robinson J, Gracey M, Lesmana M, Echeverria P, Janda JM. Hemagglutination patterns of Aeromonas spp. in relation to biotype and source. J Clin Microbiol. 1984 Jan;19(1):39–43. [PMC free article] [PubMed]
  • Burke V, Robinson J, Beaman J, Gracey M, Lesmana M, Rockhill R, Echeverria P, Janda JM. Correlation of enterotoxicity with biotype in Aeromonas spp. J Clin Microbiol. 1983 Nov;18(5):1196–1200. [PMC free article] [PubMed]
  • Cahill MM. Virulence factors in motile Aeromonas species. J Appl Bacteriol. 1990 Jul;69(1):1–16. [PubMed]
  • Callister SM, Agger WA. Enumeration and characterization of Aeromonas hydrophila and Aeromonas caviae isolated from grocery store produce. Appl Environ Microbiol. 1987 Feb;53(2):249–253. [PMC free article] [PubMed]
  • Chakraborty T, Montenegro MA, Sanyal SC, Helmuth R, Bulling E, Timmis KN. Cloning of enterotoxin gene from Aeromonas hydrophila provides conclusive evidence of production of a cytotonic enterotoxin. Infect Immun. 1984 Nov;46(2):435–441. [PMC free article] [PubMed]
  • Crichton PB, Walker JW. Methods for the detection of haemagglutinins in Aeromonas. J Med Microbiol. 1985 Apr;19(2):273–277. [PubMed]
  • Janda JM. Recent advances in the study of the taxonomy, pathogenicity, and infectious syndromes associated with the genus Aeromonas. Clin Microbiol Rev. 1991 Oct;4(4):397–410. [PMC free article] [PubMed]
  • Research on Aeromonas and Plesiomonas. IV. Virulence factors. Experientia. 1987 Apr 15;43(4):367–374. [PubMed]
  • Nishikawa Y, Kishi T. Isolation and characterization of motile Aeromonas from human, food and environmental specimens. Epidemiol Infect. 1988 Oct;101(2):213–223. [PMC free article] [PubMed]
  • Palumbo SA, Bencivengo MM, Del Corral F, Williams AC, Buchanan RL. Characterization of the Aeromonas hydrophila group isolated from retail foods of animal origin. J Clin Microbiol. 1989 May;27(5):854–859. [PMC free article] [PubMed]
  • Palumbo SA, Maxino F, Williams AC, Buchanan RL, Thayer DW. Starch-Ampicillin Agar for the Quantitative Detection of Aeromonas hydrophila. Appl Environ Microbiol. 1985 Oct;50(4):1027–1030. [PMC free article] [PubMed]
  • Turnbull PC, Lee JV, Miliotis MD, Van de Walle S, Koornhof HJ, Jeffery L, Bryant TN. Enterotoxin production in relation to taxonomic grouping and source of isolation of Aeromonas species. J Clin Microbiol. 1984 Feb;19(2):175–180. [PMC free article] [PubMed]

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