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Appl Environ Microbiol. 1996 Jul; 62(7): 2244–2251.
PMCID: PMC168005

A mannose-specific adherence mechanism in Lactobacillus plantarum conferring binding to the human colonic cell line HT-29.


Two Lactobacillus plantarum strains of human intestinal origin, strains 299 (= DSM 6595) and 299v (= DSM 9843), have proved to be efficient colonizers of the human intestine under experimental conditions. These strains and 17 other L. plantarum strains were tested for the ability to adhere to cells of the human colonic cell line HT-29.L.plantarum 299 and 299v and nine other L. plantarum strains, including all six strains that belong to the same genetic subgroup as L. plantarum 299 and 299v, adhered to HT-29 cells in a manner that could be inhibited by methyl-alpha-D-mannoside. The ability to adhere to HT-29 cells correlated with an ability to agglutinate cells of Saccharomyces cerevisiae and erythrocytes in a mannose-sensitive manner and with adherence to D-mannose-coated agarose beads. L. plantarum 299 and 299v adhered to freshly isolated human colonic and ileal enterocytes, but the binding was not significantly inhibited by methyl-alpha-D-mannoside. Periodate treatment of HT-29 cells abolished mannose-sensitive adherence, confirming that the cell-bound receptor was of carbohydrate nature. Proteinase K treatment of the bacteria also abolished adherence, indicating that the binding involved protein structures on the bacterial cell surface. Thus, a mannose-specific adhesin has been identified in L. plantarum; this adhesin could be involved in the ability to colonize the intestine.

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  • Aslanzadeh J, Paulissen LJ. Role of type 1 and type 3 fimbriae on the adherence and pathogenesis of Salmonella enteritidis in mice. Microbiol Immunol. 1992;36(4):351–359. [PubMed]
  • Bennet R, Nord CE. Development of the faecal anaerobic microflora after caesarean section and treatment with antibiotics in newborn infants. Infection. 1987;15(5):332–336. [PubMed]
  • Bernet MF, Brassart D, Neeser JR, Servin AL. Lactobacillus acidophilus LA 1 binds to cultured human intestinal cell lines and inhibits cell attachment and cell invasion by enterovirulent bacteria. Gut. 1994 Apr;35(4):483–489. [PMC free article] [PubMed]
  • Bettelheim KA, Taylor J. A study of Escherichia coli isolated from chronic urinary infection. J Med Microbiol. 1969 Aug;2(3):225–236. [PubMed]
  • Bhattacharjee JW, Srivastava BS. Mannose-sensitive haemagglutinins in adherence of Vibrio cholerae eltor to intestine. J Gen Microbiol. 1978 Aug;107(2):407–410. [PubMed]
  • Chauvière G, Coconnier MH, Kernéis S, Fourniat J, Servin AL. Adhesion of human Lactobacillus acidophilus strain LB to human enterocyte-like Caco-2 cells. J Gen Microbiol. 1992 Aug;138(Pt 8):1689–1696. [PubMed]
  • Coconnier MH, Klaenhammer TR, Kernéis S, Bernet MF, Servin AL. Protein-mediated adhesion of Lactobacillus acidophilus BG2FO4 on human enterocyte and mucus-secreting cell lines in culture. Appl Environ Microbiol. 1992 Jun;58(6):2034–2039. [PMC free article] [PubMed]
  • Duguid JP, Anderson ES, Campbell I. Fimbriae and adhesive properties in Salmonellae. J Pathol Bacteriol. 1966 Jul;92(1):107–138. [PubMed]
  • Firon N, Ofek I, Sharon N. Interaction of mannose-containing oligosaccharides with the fimbrial lectin of Escherichia coli. Biochem Biophys Res Commun. 1982 Apr 29;105(4):1426–1432. [PubMed]
  • Firon N, Ofek I, Sharon N. Carbohydrate specificity of the surface lectins of Escherichia coli, Klebsiella pneumoniae, and Salmonella typhimurium. Carbohydr Res. 1983 Aug 16;120:235–249. [PubMed]
  • Greene JD, Klaenhammer TR. Factors involved in adherence of lactobacilli to human Caco-2 cells. Appl Environ Microbiol. 1994 Dec;60(12):4487–4494. [PMC free article] [PubMed]
  • Hagberg L, Hull R, Hull S, Falkow S, Freter R, Svanborg Edén C. Contribution of adhesion to bacterial persistence in the mouse urinary tract. Infect Immun. 1983 Apr;40(1):265–272. [PMC free article] [PubMed]
  • Herías MV, Midtvedt T, Hanson LA, Wold AE. Role of Escherichia coli P fimbriae in intestinal colonization in gnotobiotic rats. Infect Immun. 1995 Dec;63(12):4781–4789. [PMC free article] [PubMed]
  • Herscovics A, Orlean P. Glycoprotein biosynthesis in yeast. FASEB J. 1993 Apr 1;7(6):540–550. [PubMed]
  • Johansson ML, Molin G, Jeppsson B, Nobaek S, Ahrné S, Bengmark S. Administration of different Lactobacillus strains in fermented oatmeal soup: in vivo colonization of human intestinal mucosa and effect on the indigenous flora. Appl Environ Microbiol. 1993 Jan;59(1):15–20. [PMC free article] [PubMed]
  • Johansson ML, Sanni A, Lönner C, Molin G. Phenotypically based taxonomy using API 50CH of lactobacilli from Nigerian ogi, and the occurrence of starch fermenting strains. Int J Food Microbiol. 1995 Apr;25(2):159–168. [PubMed]
  • Johnson JR. Virulence factors in Escherichia coli urinary tract infection. Clin Microbiol Rev. 1991 Jan;4(1):80–128. [PMC free article] [PubMed]
  • Karlsson KA. Animal glycosphingolipids as membrane attachment sites for bacteria. Annu Rev Biochem. 1989;58:309–350. [PubMed]
  • Knutton S, Lloyd DR, Candy DC, McNeish AS. In vitro adhesion of enterotoxigenic Escherichia coli to human intestinal epithelial cells from mucosal biopsies. Infect Immun. 1984 May;44(2):514–518. [PMC free article] [PubMed]
  • Krogfelt KA, McCormick BA, Burghoff RL, Laux DC, Cohen PS. Expression of Escherichia coli F-18 type 1 fimbriae in the streptomycin-treated mouse large intestine. Infect Immun. 1991 Apr;59(4):1567–1568. [PMC free article] [PubMed]
  • Lindquist BL, Lebenthal E, Lee PC, Stinson MW, Merrick JM. Adherence of Salmonella typhimurium to small-intestinal enterocytes of the rat. Infect Immun. 1987 Dec;55(12):3044–3050. [PMC free article] [PubMed]
  • Mirelman D, Altmann G, Eshdat Y. Screening of bacterial isolates for mannose-specific lectin activity by agglutination of yeasts. J Clin Microbiol. 1980 Apr;11(4):328–331. [PMC free article] [PubMed]
  • Molin G, Jeppsson B, Johansson ML, Ahrné S, Nobaek S, Ståhl M, Bengmark S. Numerical taxonomy of Lactobacillus spp. associated with healthy and diseased mucosa of the human intestines. J Appl Bacteriol. 1993 Mar;74(3):314–323. [PubMed]
  • Neeser JR, Koellreutter B, Wuersch P. Oligomannoside-type glycopeptides inhibiting adhesion of Escherichia coli strains mediated by type 1 pili: preparation of potent inhibitors from plant glycoproteins. Infect Immun. 1986 May;52(2):428–436. [PMC free article] [PubMed]
  • Ofek I, Mirelman D, Sharon N. Adherence of Escherichia coli to human mucosal cells mediated by mannose receptors. Nature. 1977 Feb 17;265(5595):623–625. [PubMed]
  • Ogier-Denis E, Codogno P, Chantret I, Trugnan G. The processing of asparagine-linked oligosaccharides in HT-29 cells is a function of their state of enterocytic differentiation. An accumulation of Man9,8-GlcNAc2-Asn species is indicative of an impaired N-glycan trimming in undifferentiated cells. J Biol Chem. 1988 May 5;263(13):6031–6037. [PubMed]
  • Reid G, Servin AL, Bruce AW, Busscher HJ. Adhesion of three Lactobacillus strains to human urinary and intestinal epithelial cells. Microbios. 1993;75(302):57–65. [PubMed]
  • ROGOSA M, MITCHELL JA, WISEMAN RF. A selective medium for the isolation and enumeration of oral and fecal lactobacilli. J Bacteriol. 1951 Jul;62(1):132–133. [PMC free article] [PubMed]
  • Sanchez J, Jonson G. Binding of bacteria to carbohydrates immobilized on beads to demonstrate the presence of cell-associated hemagglutinins in Vibrio cholerae. APMIS. 1990 Apr;98(4):353–357. [PubMed]
  • Spencer JF, Gorin PA. Mannose-containing polysaccharides of yeasts. Biotechnol Bioeng. 1973 Jan;15(1):1–12. [PubMed]
  • Wadolkowski EA, Laux DC, Cohen PS. Colonization of the streptomycin-treated mouse large intestine by a human fecal Escherichia coli strain: role of adhesion to mucosal receptors. Infect Immun. 1988 May;56(5):1036–1043. [PMC free article] [PubMed]
  • Weiser MM. Intestinal epithelial cell surface membrane glycoprotein synthesis. I. An indicator of cellular differentiation. J Biol Chem. 1973 Apr 10;248(7):2536–2541. [PubMed]
  • Wold AE, Mestecky J, Tomana M, Kobata A, Ohbayashi H, Endo T, Edén CS. Secretory immunoglobulin A carries oligosaccharide receptors for Escherichia coli type 1 fimbrial lectin. Infect Immun. 1990 Sep;58(9):3073–3077. [PMC free article] [PubMed]
  • Wold AE, Thorssén M, Hull S, Edén CS. Attachment of Escherichia coli via mannose- or Gal alpha 1----4Gal beta-containing receptors to human colonic epithelial cells. Infect Immun. 1988 Oct;56(10):2531–2537. [PMC free article] [PubMed]
  • Yamamoto T, Yokota T. Electron microscopic study of Vibrio cholerae O1 adherence to the mucus coat and villus surface in the human small intestine. Infect Immun. 1988 Oct;56(10):2753–2759. [PMC free article] [PubMed]

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