• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of aemPermissionsJournals.ASM.orgJournalAEM ArticleJournal InfoAuthorsReviewers
Appl Environ Microbiol. Jun 1996; 62(6): 1958–1963.
PMCID: PMC167974

Inhibition of initial adhesion of uropathogenic Enterococcus faecalis by biosurfactants from Lactobacillus isolates.


In this study, 15 Lactobacillus isolates were found to produce biosurfactants in the mid-exponential and stationary growth phases. The stationary-phase biosurfactants from lactobacillus casei subsp. rhamnosus 36 and ATCC 7469, Lactobacillus fermentum B54, and Lactobacillus acidophilus RC14 were investigated further to determine their capacity to inhibit the initial adhesion of uropathogenic Enterococcus faecalis 1131 to glass in a parallel-plate flow chamber. The initial deposition rate of E. faecalis to glass with an adsorbed biosurfactant layer from L. acidophilus RC14 or L. fermentum B54 was significantly decreased by approximately 70%, while the number of adhering enterococci after 4 h of adhesion was reduced by an average of 77%. The surface activity of the biosurfactants and their activity inhibiting the initial adhesion of E. faecalis 1131 were retained after dialysis (molecular weight cutoff, 6,000 to 8,000) and freeze-drying. Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy revealed that the freeze-dried biosurfactants from L. acidophilus RC14 and L. fermentum B54 were richest in protein, while those from L. casei subsp. rhamnosus 36 and ATCC 7469 had relatively high polysaccharide and phosphate contents.

Full Text

The Full Text of this article is available as a PDF (296K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Abee T, Klaenhammer TR, Letellier L. Kinetic studies of the action of lactacin F, a bacteriocin produced by Lactobacillus johnsonii that forms poration complexes in the cytoplasmic membrane. Appl Environ Microbiol. 1994 Mar;60(3):1006–1013. [PMC free article] [PubMed]
  • Blomberg L, Henriksson A, Conway PL. Inhibition of adhesion of Escherichia coli K88 to piglet ileal mucus by Lactobacillus spp. Appl Environ Microbiol. 1993 Jan;59(1):34–39. [PMC free article] [PubMed]
  • Bruce AW, Reid G. Intravaginal instillation of lactobacilli for prevention of recurrent urinary tract infections. Can J Microbiol. 1988 Mar;34(3):339–343. [PubMed]
  • Chan RC, Bruce AW, Reid G. Adherence of cervical, vaginal and distal urethral normal microbial flora to human uroepithelial cells and the inhibition of adherence of gram-negative uropathogens by competitive exclusion. J Urol. 1984 Mar;131(3):596–601. [PubMed]
  • Chan RC, Reid G, Irvin RT, Bruce AW, Costerton JW. Competitive exclusion of uropathogens from human uroepithelial cells by Lactobacillus whole cells and cell wall fragments. Infect Immun. 1985 Jan;47(1):84–89. [PMC free article] [PubMed]
  • Cirigliano MC, Carman GM. Purification and Characterization of Liposan, a Bioemulsifier from Candida lipolytica. Appl Environ Microbiol. 1985 Oct;50(4):846–850. [PMC free article] [PubMed]
  • Cowan MM, van der Mei HC, Rouxhet PG, Busscher HJ. Physico-chemical and structural properties of the surfaces of Peptostreptococcus micros and Streptococcus mitis as compared to those of mutans streptococci, Streptococcus sanguis and Streptococcus salivarius. J Gen Microbiol. 1992 Dec;138(12):2707–2714. [PubMed]
  • Fiechter A. Biosurfactants: moving towards industrial application. Trends Biotechnol. 1992 Jun;10(6):208–217. [PubMed]
  • Klaenhammer TR. Bacteriocins of lactic acid bacteria. Biochimie. 1988 Mar;70(3):337–349. [PubMed]
  • Kunin CM, Polyak F, Postel E. Periurethral bacterial flora in women. Prolonged intermittent colonization with Escherichia coli. JAMA. 1980 Jan 11;243(2):134–139. [PubMed]
  • Lin SC, Minton MA, Sharma MM, Georgiou G. Structural and immunological characterization of a biosurfactant produced by Bacillus licheniformis JF-2. Appl Environ Microbiol. 1994 Jan;60(1):31–38. [PMC free article] [PubMed]
  • Marrie TJ, Swantee CA, Hartlen M. Aerobic and anaerobic urethral flora of healthy females in various physiological age groups and of females with urinary tract infections. J Clin Microbiol. 1980 Jun;11(6):654–659. [PMC free article] [PubMed]
  • McGroarty JA, Reid G. Detection of a Lactobacillus substance that inhibits Escherichia coli. Can J Microbiol. 1988 Aug;34(8):974–978. [PubMed]
  • Millsap K, Reid G, van der Mei HC, Busscher HJ. Displacement of Enterococcus faecalis from hydrophobic and hydrophilic substrata by Lactobacillus and Streptococcus spp. as studied in a parallel plate flow chamber. Appl Environ Microbiol. 1994 Jun;60(6):1867–1874. [PMC free article] [PubMed]
  • Mortvedt-Abildgaa CI, Nissen-Meyer J, Jelle B, Grenov B, Skaugen M, Nes IF. Production and pH-Dependent Bactericidal Activity of Lactocin S, a Lantibiotic from Lactobacillus sake L45. Appl Environ Microbiol. 1995 Jan;61(1):175–179. [PMC free article] [PubMed]
  • Nagy E, Petterson M, Mårdh PA. Antibiosis between bacteria isolated from the vagina of women with and without signs of bacterial vaginosis. APMIS. 1991 Aug;99(8):739–744. [PubMed]
  • Pfau A, Sacks T. The bacterial flora of the vaginal vestibule, urethra and vagina in premenopausal women with recurrent urinary tract infections. J Urol. 1981 Nov;126(5):630–634. [PubMed]
  • Preston CA, Bruce AW, Reid G. Antibiotic resistance of urinary pathogens isolated from patients attending the Toronto Hospital between 1986 and 1990. J Hosp Infect. 1992 Oct;22(2):129–135. [PubMed]
  • Redondo-Lopez V, Cook RL, Sobel JD. Emerging role of lactobacilli in the control and maintenance of the vaginal bacterial microflora. Rev Infect Dis. 1990 Sep-Oct;12(5):856–872. [PubMed]
  • Reid G, Cook RL, Bruce AW. Examination of strains of lactobacilli for properties that may influence bacterial interference in the urinary tract. J Urol. 1987 Aug;138(2):330–335. [PubMed]
  • Reid G, Cuperus PL, Bruce AW, van der Mei HC, Tomeczek L, Khoury AH, Busscher HJ. Comparison of contact angles and adhesion to hexadecane of urogenital, dairy, and poultry lactobacilli: effect of serial culture passages. Appl Environ Microbiol. 1992 May;58(5):1549–1553. [PMC free article] [PubMed]
  • Reid G, Lam D, Bruce AW, van der Mei HC, Busscher HJ. Adhesion of lactobacilli to urinary catheters and diapers: effect of surface properties. J Biomed Mater Res. 1994 Jun;28(6):731–734. [PubMed]
  • Reid G, McGroarty JA, Angotti R, Cook RL. Lactobacillus inhibitor production against Escherichia coli and coaggregation ability with uropathogens. Can J Microbiol. 1988 Mar;34(3):344–351. [PubMed]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...