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Appl Environ Microbiol. May 1996; 62(5): 1741–1746.
PMCID: PMC167948

Distribution of sewage indicated by Clostridium perfringens at a deep-water disposal site after cessation of sewage disposal.


Clostridium perfringens, a marker of domestic sewage contamination, was enumerated in sediment samples obtained from the vicinity of the 106-Mile Site 1 month and 1 year after cessation of sewage disposal at this site. C. perfringens counts in sediments collected at the disposal site and from stations 26 nautical miles (ca. 48 km) and 50 nautical miles (ca. 92 km) to the southwest of the site were, in general, more than 10-fold higher than counts from an uncontaminated reference site. C. perfringens counts at the disposal site were not significantly different between 1992 and 1993, suggesting that sewage sludge had remained in the benthic environment at this site. At stations where C. perfringens counts were elevated (i.e., stations other than the reference station), counts were generally higher in the top 1 cm and decreased down to 5 cm. In some cases, C. perfringens counts in the bottom 4 or 5 cm showed a trend of higher counts in 1993 than in 1992, suggesting bioturbation. We conclude that widespread sludge contamination of the benthic environment has persisted for at least 1 year after cessation of ocean sewage disposal at the 106-Mile Site.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Armon R, Payment P. A modified m-CP medium for enumerating Clostridium perfringens from water samples. Can J Microbiol. 1988 Jan;34(1):78–79. [PubMed]
  • Bisson JW, Cabelli VJ. Membrane filter enumeration method for Clostridium perfringens. Appl Environ Microbiol. 1979 Jan;37(1):55–66. [PMC free article] [PubMed]
  • Davies CM, Long JA, Donald M, Ashbolt NJ. Survival of fecal microorganisms in marine and freshwater sediments. Appl Environ Microbiol. 1995 May;61(5):1888–1896. [PMC free article] [PubMed]
  • Avies JA. Isolation and identification of clostridia from North Sea sediments. J Appl Bacteriol. 1969 Jun;32(2):164–169. [PubMed]
  • Hill RT, Knight IT, Anikis MS, Colwell RR. Benthic Distribution of Sewage Sludge Indicated by Clostridium perfringens at a Deep-Ocean Dump Site. Appl Environ Microbiol. 1993 Jan;59(1):47–51. [PMC free article] [PubMed]
  • Takizawa M, Straube WL, Hill RT, Colwell RR. Near-bottom pelagic bacteria at a deep-water sewage sludge disposal site. Appl Environ Microbiol. 1993 Oct;59(10):3406–3410. [PMC free article] [PubMed]
  • Aseeva EA, Lutsenko IN, Pivnik AV, Spivak VA, Beliaeva LS, Kremenetskaia AM, Saad V. Pervyi sluchai nositel'stva Hb Camden v Rossii. Ter Arkh. 1993;65(7):49–51. [PubMed]
  • Wood AJ, Trust TJ. Some qualitative and quantitative aspects of the intestinal microflora of the glaucous-winged gull (Larus glaucescens). Can J Microbiol. 1972 Oct;18(10):1577–1583. [PubMed]

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