• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of aemPermissionsJournals.ASM.orgJournalAEM ArticleJournal InfoAuthorsReviewers
Appl Environ Microbiol. Apr 1996; 62(4): 1242–1247.
PMCID: PMC167889

PCR detection and quantitation of predominant anaerobic bacteria in human and animal fecal samples.

Abstract

PCR procedures based on 16S rRNA gene sequences specific for 12 anaerobic bacteria that predominate in the human intestinal tract were developed and used for quantitative detection of these species in human (adult and baby) feces and animal (rat, mouse, cat, dog, monkey, and rabbit) feces. Fusobacterium prausnitzii, Peptostreptococcus productus, and Clostridium clostridiiforme had high PCR titers (the maximum dilutions for positive PCR results ranged from 10(-3) to 10(-8)) in all of the human and animal fecal samples tested. Bacteroides thetaiotaomicron, Bacteroides vulgatus, and Eubacterium limosum also showed higher PCR titers (10(-2) to 10(-6)) in adult human feces. The other bacteria tested, including Escherichia coli, Bifidobacterium adolescentis, Bifidobacterium longum, Lactobacillus acidophilus, Eubacterium biforme, and Bacteroides distasonis, were either at low PCR titers (less than 10(-2)) or not detected by PCR. The reported PCR procedure including the fecal sample preparation method is simplified and rapid and eliminates the DNA isolation steps.

Full Text

The Full Text of this article is available as a PDF (285K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol. 1990 Oct 5;215(3):403–410. [PubMed]
  • Aries V, Crowther JS, Drasar BS, Hill MJ, Williams RE. Bacteria and the aetiology of cancer of the large bowel. Gut. 1969 May;10(5):334–335. [PMC free article] [PubMed]
  • Bej AK, Steffan RJ, DiCesare J, Haff L, Atlas RM. Detection of coliform bacteria in water by polymerase chain reaction and gene probes. Appl Environ Microbiol. 1990 Feb;56(2):307–314. [PMC free article] [PubMed]
  • Candrian U, Furrer B, Höfelein C, Meyer R, Jermini M, Lüthy J. Detection of Escherichia coli and identification of enterotoxigenic strains by primer-directed enzymatic amplification of specific DNA sequences. Int J Food Microbiol. 1991 Apr;12(4):339–351. [PubMed]
  • Cerniglia CE, Freeman JP, Franklin W, Pack LD. Metabolism of azo dyes derived from benzidine, 3,3'-dimethyl-benzidine and 3,3'-dimethoxybenzidine to potentially carcinogenic aromatic amines by intestinal bacteria. Carcinogenesis. 1982;3(11):1255–1260. [PubMed]
  • Cerniglia CE, Howard PC, Fu PP, Franklin W. Metabolism of nitropolycyclic aromatic hydrocarbons by human intestinal microflora. Biochem Biophys Res Commun. 1984 Aug 30;123(1):262–270. [PubMed]
  • Chadwick RW, George SE, Claxton LD. Role of the gastrointestinal mucosa and microflora in the bioactivation of dietary and environmental mutagens or carcinogens. Drug Metab Rev. 1992;24(4):425–492. [PubMed]
  • Hill MJ, Goddard P, Williams RE. Gut bacteria and aetiology of cancer of the breast. Lancet. 1971 Aug 28;2(7722):472–473. [PubMed]
  • Kreader CA. Design and evaluation of Bacteroides DNA probes for the specific detection of human fecal pollution. Appl Environ Microbiol. 1995 Apr;61(4):1171–1179. [PMC free article] [PubMed]
  • McDonald JJ, Cerniglia CE. Biotransformation of gentian violet to leucogentian violet by human, rat, and chicken intestinal microflora. Drug Metab Dispos. 1984 May-Jun;12(3):330–336. [PubMed]
  • Moore WE, Holdeman LV. Human fecal flora: the normal flora of 20 Japanese-Hawaiians. Appl Microbiol. 1974 May;27(5):961–979. [PMC free article] [PubMed]
  • Moore WE, Moore LH. Intestinal floras of populations that have a high risk of colon cancer. Appl Environ Microbiol. 1995 Sep;61(9):3202–3207. [PMC free article] [PubMed]
  • Slavik MF, Wang RF, Cao WW. Development and evaluation of the polymerase chain reaction method for diagnosis of Mycoplasma gallisepticum infection in chickens. Mol Cell Probes. 1993 Dec;7(6):459–463. [PubMed]
  • Rong-Fu W, Beasley JN, Cao WW, Slavik MF, Johnson MG. Development of PCR method specific for Marek's disease virus. Mol Cell Probes. 1993 Apr;7(2):127–131. [PubMed]
  • Wang RF, Cao WW, Campbell WL, Hairston L, Franklin W, Cerniglia CE. The use of PCR to monitor the population abundance of six human intestinal bacterial species in an in vitro semicontinuous culture system. FEMS Microbiol Lett. 1994 Dec 1;124(2):229–237. [PubMed]
  • Wang RF, Cao WW, Franklin W, Campbell W, Cerniglia CE. A 16S rDNA-based PCR method for rapid and specific detection of Clostridium perfringens in food. Mol Cell Probes. 1994 Apr;8(2):131–137. [PubMed]
  • Wang RF, Cao WW, Johnson MG. Development of a 16S rRNA-based oligomer probe specific for Listeria monocytogenes. Appl Environ Microbiol. 1991 Dec;57(12):3666–3670. [PMC free article] [PubMed]
  • Wang RF, Cao WW, Johnson MG. 16S rRNA-based probes and polymerase chain reaction method to detect Listeria monocytogenes cells added to foods. Appl Environ Microbiol. 1992 Sep;58(9):2827–2831. [PMC free article] [PubMed]
  • Wang RF, Cao WW, Wang H, Johnson MG. A 16S rRNA-based DNA probe and PCR method specific for Listeria ivanovii. FEMS Microbiol Lett. 1993 Jan 1;106(1):85–92. [PubMed]
  • Wilde J, Eiden J, Yolken R. Removal of inhibitory substances from human fecal specimens for detection of group A rotaviruses by reverse transcriptase and polymerase chain reactions. J Clin Microbiol. 1990 Jun;28(6):1300–1307. [PMC free article] [PubMed]

Articles from Applied and Environmental Microbiology are provided here courtesy of American Society for Microbiology (ASM)

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...