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Antimicrob Agents Chemother. Nov 1996; 40(11): 2542–2544.
PMCID: PMC163571

Integration of pT181-like tetracycline resistance plasmids into large staphylococcal plasmids involves IS257.

Abstract

Four large staphylococcal plasmids ranging in size from 31 to 82 kbp have been shown to mediate tetracycline resistance via an integrated copy of the tet(K)-encoding plasmid pT181 which was flanked by copies of the insertion element IS257. In two cases, IS257 elements interrupted the repC reading frame of pT181 and an 8-bp sequence from within the repC gene was duplicated at the interrupted site. In the third plasmid, the IS257 elements interrupted the pT181 DNA immediately upstream of the repC coding sequence with an 8-bp duplication. In the fourth case, the IS257 elements flanked a pT181-like plasmid with one IS257 in the repC coding sequence and the other within the recombinase (pre) coding sequence, so that a section of the pT181 sequence was deleted. All four integration sites detected in this study differ from those previously described for the IS257-mediated integration of pT181-like plasmids into large plasmids or into the chromosomal DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Ferretti JJ, Gilmore KS, Courvalin P. Nucleotide sequence analysis of the gene specifying the bifunctional 6'-aminoglycoside acetyltransferase 2"-aminoglycoside phosphotransferase enzyme in Streptococcus faecalis and identification and cloning of gene regions specifying the two activities. J Bacteriol. 1986 Aug;167(2):631–638. [PMC free article] [PubMed]
  • Khan SA, Novick RP. Complete nucleotide sequence of pT181, a tetracycline-resistance plasmid from Staphylococcus aureus. Plasmid. 1983 Nov;10(3):251–259. [PubMed]
  • Lodder G, Schwarz S, Gregory P, Dyke K. Tandem duplication in ermC translational attenuator of the macrolide-lincosamide-streptogramin B resistance plasmid pSES6 from Staphylococcus equorum. Antimicrob Agents Chemother. 1996 Jan;40(1):215–217. [PMC free article] [PubMed]
  • Needham C, Noble WC, Dyke KG. The staphylococcal insertion sequence IS257 is active. Plasmid. 1995 Nov;34(3):198–205. [PubMed]
  • Needham C, Rahman M, Dyke KG, Noble WC. An investigation of plasmids from Staphylococcus aureus that mediate resistance to mupirocin and tetracycline. Microbiology. 1994 Oct;140(Pt 10):2577–2583. [PubMed]
  • Rahman M, Noble WC, Dyke KG. Probes for the study of mupirocin resistance in staphylococci. J Med Microbiol. 1993 Dec;39(6):446–449. [PubMed]
  • Roberts MC. Epidemiology of tetracycline-resistance determinants. Trends Microbiol. 1994 Oct;2(10):353–357. [PubMed]
  • Rouch DA, Skurray RA. IS257 from Staphylococcus aureus: member of an insertion sequence superfamily prevalent among gram-positive and gram-negative bacteria. Gene. 1989;76(2):195–205. [PubMed]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PMC free article] [PubMed]
  • Schwarz S, Noble WC. Tetracycline resistance genes in staphylococci from the skin of pigs. J Appl Bacteriol. 1994 Apr;76(4):320–326. [PubMed]
  • Schwarz S, Spies U, Cardoso M. Cloning and sequence analysis of a plasmid-encoded chloramphenicol acetyltransferase gene from Staphylococcus intermedius. J Gen Microbiol. 1991 Apr;137(4):977–981. [PubMed]
  • Stewart PR, Dubin DT, Chikramane SG, Inglis B, Matthews PR, Poston SM. IS257 and small plasmid insertions in the mec region of the chromosome of Staphylococcus aureus. Plasmid. 1994 Jan;31(1):12–20. [PubMed]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

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