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Proc Biol Sci. Aug 7, 2006; 273(1596): 1857–1866.
Published online Apr 28, 2006. doi:  10.1098/rspb.2006.3536
PMCID: PMC1634797

Arthropod phylogeny: onychophoran brain organization suggests an archaic relationship with a chelicerate stem lineage

Abstract

Neuroanatomical studies have demonstrated that the architecture and organization among neuropils are highly conserved within any order of arthropods. The shapes of nerve cells and their neuropilar arrangements provide robust characters for phylogenetic analyses. Such analyses so far have agreed with molecular phylogenies in demonstrating that entomostracans+malacostracans belong to a clade (Tetraconata) that includes the hexapods. However, relationships among what are considered to be paraphyletic groups or among the stem arthropods have not yet been satisfactorily resolved. The present parsimony analyses of independent neuroarchitectural characters from 27 arthropods and lobopods demonstrate relationships that are congruent with phylogenies derived from molecular studies, except for the status of the Onychophora. The present account describes the brain of the onychophoran Euperipatoides rowelli, demonstrating that the structure and arrangements of its neurons, cerebral neuropils and sensory centres are distinct from arrangements in the brains of mandibulates. Neuroanatomical evidence suggests that the organization of the onychophoran brain is similar to that of the brains of chelicerates.

Keywords: brain organization, arthropod evolution, Onychophora, Chelicerata

1. Introduction

The phylogenetic position of the onychophorans is considered by many to be central for understanding the origin and evolution of the arthropods. Modern taxa bear similarity to certain lower Cambrian forms (Ramsköld & Chen 1998), particularly Onychodictyon (Hou & Bergström 1995), and fossil evidence has been used to propose that extinct onychophoran taxa represent a basal lobopod zootype ancestral to arthropod lineages (Budd 1996). This placement of the Onychophora finds support from molecular characteristics (Aguinaldo et al. 1997; Colgan et al. 1998), respiratory proteins (Kusche et al. 2002) and Hox gene expression (Grenier et al. 1997). Combined molecular phylogenetics and morphological criteria generally place the Onychophora basal to the Arthropoda (Giribet et al. 2001) or as a sister group to the tardigrades and euarthropods (Zrzavy et al. 1998; Regier et al. 2005; Waloszek et al. 2005). In contrast, a study based on 12S ribosomal RNA supports the onychophorans not as basal arthropods but as modified euarthropods comprising a sister group to the chelicerates (Ballard et al. 1992).

Distinct from such analyses are recent comparisons of sensory and neural components of extant groups. Neuromorphological analyses have led to the recognition that ommatidial development (Melzer et al. 2000) and brain anatomy (Strausfeld 1998, 2005) unite the insects and crustaceans and that arrangements of cerebral neuropils show the Remipedia to be phylogenetically closer to the malacostracans and hexapods than to basal crustaceans (Fanenbruck & Harzsch 2005). In the early 1900s, it was already recognized that the organization of the arthropod visual system and central brain was so highly conserved within different arthropod groups that these features could be used to suggest phylogenetic associations. Such neurophylogenetic studies pioneered by Holmgren (1916) and Hanström (1926) concluded that insects and crustaceans were monophyletic, derived from a common ancestor, and were not sister groups of the Myriapoda. This affinity was supported 80 years later by morphological and developmental studies allowing the recognition of a clade ‘Pancrustacea’ (Hexapoda+Crustacea; Stys & Zrzavy 1994) later termed the Tetraconata on the basis of common ommatidial organization across these groups (Dohle 2001; Richter 2002). Improved technologies for studying brain fibroarchitectures and neuron morphologies have demonstrated their robustness for phylogenetic reconstruction (Loesel et al. 2002) and have provided relational trees that are congruent with those achieved from molecular studies, while questioning the monophyly of certain groups such as the ‘Myriapoda’.

Here, we employ neuroanatomical methods to describe salient features of the brain of an onychophoran and several chelicerates, to demonstrate similarities between their neuropils, and to distinguish them from brain organization in the Mandibulata. Support for a sister-group status for the Chelicerata and Onychophora is revealed by a cladistic analysis of 27 arthropod taxa employing 118 independent neuroarchitectural characters.

2. Material and methods

(a) Taxa and histological data production

Taxa used for this study were purchased or collected in Arizona, at designated sites in New South Wales, Australia, and sites belonging to the Friday Harbor Marine Biology Laboratory, Washington, USA. Giant Tasmanian neanurid collembolans were provided by Dr Penny Greenslade at the CSIRO, Canberra. Dr Roy L. Caldwell, University of California, Berkeley, provided live stomatopods (Pseudosquilla ciliata). Liphistiomorph spiders (Heptathela kimurai) were obtained from designated sites on Mount Aso, Kyushu, Japan. The true spider Cupiennius salei was obtained from breeding colonies at the University of Frankfurt. Pycnogonids (Lycythorhyncus sp.) were collected from coastal habitats near Fukuoka, Japan; Machilis germanicus from vicinities in Würzburg, Germany; and Scutigera coleoptrata near Greve, Italy. Taxa used for this account are given in figure 5 and table 1.

Figure 5
(a) The results of a strict consensus of the three most parsimonious trees. All characters (see character matrix and character list, electronic supplementary material) are treated as unordered and unweighted. All trees support a sister relationship between ...
Table 1
Ten characters relevant to midline neuropils in mandibulates, chelicerates and onychophorans with Arctonoe fragilis. Plus signs indicate the presence, minus signs the absence of a trait. Plus signs in parenthesis indicate that, in scolopendromorphs, modules ...

For histology, animals were cooled to immobility, fixed and stained by reduced silver, ethyl gallate or Golgi impregnation (Strausfeld & Li 1999). Vibratome sections were treated with primary antibodies against serotonin and an antiserum raised in mice to Diploptera punctata allatostatin I (courtesy Dr Barbara Stay, University of Iowa), secondarily labelled and reconstructed using a Zeiss Pascal confocal microscope. Silver-stained images were captured using a Sony DKC 5000 CCD digital camera linked to an Apple G4 computer. For electron microscopy, tissue was fixed by double aldehyde and osmium methods and embedded in Epon resin. Sections were viewed in JEOL 2000EX transmission electron microscopes and images recorded on an SIS Megaview digital camera.

(b) Character specification and analysis

Characters used for phylogenetic analysis are (i) architectures of circumscribed neuropils; (ii) neuron morphologies; (iii) distribution, size and clustering of neuronal somata; (iv) certain configurations of neuropils, such as optic lobe regions scored as being twice, thrice or four times nested beneath a compound retina or glomerular neuropils with compound, spherical or wedged subunits; and (v) synaptic structures.

Of particular relevance here is the organization of unpaired midline neuropils in the first brain segment (protocerebrum), their modular origin from remotely positioned or immediate somata, and the presence or absence of satellite neuropils (see Loesel et al. 2002). Descriptions of the 118 characters used for this analysis are provided in the appendix (see electronic supplementary material).

(c) Phylogenetic analyses

Characters are treated as unlinked, unordered, equally weighted and either present or absent. Characters are not assigned character states. All characters are structural entities (see electronic supplementary material) without functional affiliations. Phylogenetic relationships were analysed using PAUP (phylogenetic analysis using parsimony; Swofford 2002) using a data matrix comprising 118 neural characters recorded as present or absent in 28 invertebrate taxa, including one annelid, the polychaete scale worm Arctonoe fragilis. Neuroanatomical analysis established that all species used were representative of their wider taxonomic classes. For example, three onychophoran species (two peripatopsids, E. rowelli and Phallocephale tallagandensis and an un-named peripatid species from the Mazatlan peninsula), three chilopod species (Scutigera coleoptrata and Scolopendra alternans, Scolopendra polymorpha) and two diplopods (Archspirospreptus gigas and Orthoporus ornatus) were screened, as were representatives of three suborders of spiders, several orders of palaeopteran and neopteran insects, eumalacostracans and two entomostracans (Triops and Artemia). As there was no difference within each group with respect to the data matrix, only one representative of each was included. Parsimony analysis was carried out via a heuristic search using 1000 random stepwise addition replicates. Trees were computed as unrooted.

3. Cerebral organization

(a) Neuroanatomy: mushroom body organization and relationships with olfactory neuropils

Neural architectures were used to compare the brains of the Australian onychophoran E. rowelli with arthropod taxa and the scale worm A. fragilis. In onychophorans and chelicerates, as in insects, the most anterior brain segment is equipped with elaborate higher centres, including the paired mushroom bodies (figure 1a,c). These centres, defined by Flögel (1876), were first recognized in neopteran insects where they possess cap-like neuropils called calyces that surmount and are contiguous with a stalk (pedunculus) and a system of lobes. Mushroom bodies are here scored as present if their pedunculi and lobes are composed of dense bundles of parallel intrinsic fibres originating from dense clusters of many thousands of uniformly small basophilic perikarya called globuli cells (Kenyon 1896).

Figure 1
Brain regions shared by onychophorans ((a, b) Euperipatoides rowelli) and chelicerates ((c, d) Eremobates pallipes). (a, c) The paired mushroom body (MB) calyces (Ca), lobes (lo) and heterolateral commissures (com) shown green. VNC, ventral nerve cord. ...

Mushroom bodies are common to euarthropods and polychaetes and are thus likely to be symplesiomorphic, possessed by the last common ancestor of annelids and arthropods. Additional and specific neuroanatomical features of mushroom bodies characterize specific arthropod taxa as do the organization and location of primary olfactory neuropils associated with them. Mushroom bodies in chelicerates can have several lobe systems: lateral lobes that extend postero-ventrally through the protocerebrum; short vertical lobes that extend downwards from the globuli cells; and heterolateral lobes that extend across the midline, linking these neuropils in each side of the brain. In amblypygids, the lateral lobes are enormously extended and folded at the expense of the heterolateral and vertical lobes (Strausfeld et al. 1998). In Limulus, the short vertical lobes are reiterated many times at the expense of the lateral and heterolateral lobes. As a consequence, in xiphosurans much of the brain is filled with short pedunculus-like assemblies of parallel fibres that reach ventrally from a dorsal rind comprising millions of globuli cells (Fahrenbach & Chamberlain 1987). Heterolateral lobes are pronounced in araneans (Strausfeld & Barth 1993), and in the present study have been confirmed in Delana cancerides, H. kimurai and in salticids.

Histological preparations of brains of E. rowelli reveal paired lobed neuropils (figure 1a) that conform to the Flögel–Kenyon criteria for mushroom bodies and show structural similarity with mushroom bodies of chelicerates. In E. rowelli, there are three lateral lobes, a more medial vertical lobe and a system of heterolateral lobes. Each lobe comprises many hundreds of parallel fibres derived from globuli cells lying dorsally over the brain. Contiguity of one lobe system across the midline, connecting the mushroom bodies on both sides, was also described by Holmgren (1916) and Schürmann (1987) and is typical of some chelicerates, here exemplified by the solfugid Eremobates pallipes (figure 1c).

Olfactory appendages provide chemosensory axons to glomerular olfactory lobes. With few exceptions, such as in isopods where mushroom bodies are lacking or araneans where mushroom bodies are supplied by second-order visual neuropils (Strausfeld & Barth 1993), olfactory glomeruli are connected by relay neurons to the mushroom bodies or, in malacostracan crustaceans, to the hemiellipsoid bodies thought to be equivalent to the mushroom bodies (Strausfeld et al. 1995; McKinzie et al. 2003). However, the arrangements of first-order olfactory neuropils in mandibulates and chelicerates show clear differences. In mandibulates, olfactory glomeruli are located in the deutocerebrum, the second preoral neuromere of the brain. In malacostracans, olfactory lobe glomeruli are supplied by receptor axons from the antennules (first antennae) and are connected by relays bilaterally to the hemiellipsoid bodies. In insects, antennal lobe relay neurons extend ipsilaterally to the mushroom body calyces. In chelicerates, although the mushroom bodies are in the protocerebrum, the olfactory glomeruli occur in the neuromere of whichever segment provides an appendage equipped with olfactory receptors. Relay neurons from glomeruli ascend ipsilaterally to the mushroom bodies. Olfactory appendages can be the pedipalps (in solfugids), the first leg pair (amblypygid, uropygids) or every leg pair as in pycnogonids. In the Onychophora, glomeruli are located in the protocerebrum as it is this brain segment that is supplied with sensory axons from the pair of frontal appendages (‘antennae’; Eriksson et al. 2003). The presence of these appendages, which have been suggested to be modified legs (Mayer & Koch 2005), attests to the ancestral status of this taxon notwithstanding its proposed relationship with the chelicerates discussed below. In the Onychophora, relay neurons extend from the antennal glomeruli to the distal region of the ipsilateral mushroom body (figure 1a).

A microanatomical feature shared by the mushroom bodies of chelicerates and E. rowelli is seen at the boundary of the pedunculus and the lateral lobes arising from it. In E. rowelli, this location reveals elaborate synaptic structures comprising extensive membrane densities (figure 1e,f) that closely resemble synapses at the equivalent location in the opilionid Holonuncia sp. (figure 1g). Synapses at an equivalent location in insects have small and discrete presynaptic densities such as in the mushroom body of the honey bee Apis mellifera (inset to figure 1e).

(b) Neuroanatomy: organization of midline neuropils

In all euarthropods other than diplopods, the protocerebrum possesses a distinctive midline neuropil. In mandibulates, this neuropil differs from that of chelicerates and onychophorans. In mandibulate brains, the midline neuropil is embedded between the two protocerebral hemispheres (figure 2cj), from which it recruits afferents and to which it provides systems of efferent neurons that extend to descending pathways. Its modular organization derives from heterolateral neurons, the cell bodies of which are remote from the neuropil (figure 2cj). In malacostracan crustaceans, including stomatopods, and pterygote insects, midline neuropils are elaborated into a complex of three discrete but interconnected parts: the superior arch, fan-shaped body and ellipsoid body. These unpaired neuropils are connected to paired satellite regions that are most numerous and elaborate in hexapod crown taxa (Loesel et al. 2002). The crescent- or arch-shaped midline neuropil of chelicerates (termed the arcuate body) extends across the protocerebrum superficially (figures 1d and 3bh) rather than being embedded within it. Arcuate bodies do not form complexes with discrete satellite neuropils but comprise numerous and elaborate strata. These derive from columnar modules that are provided by hundreds (as in small opilionids) or thousands (as in ctenid spiders and solfugids) of perpendicular neurons supplied by perikarya lying immediately above or posterior to the neuropil. Species–specific differences of arcuate bodies relate to the breadth and depth of the neuropil, and the spacing of its strata (figure 3bh). Such a central organization of columnar neurons is found in all chelicerates. Arcuate bodies also characterize the onychophoran brain (figures 1b, 3a, 4a,d,e). As in chelicerates, the onychophoran arcuate body is situated dorsally athwart the protocerebrum. Its relationship with the visual system and its internal architecture is like that of the chelicerate arcuate body. In E. rowelli (figure 1b), as in solfugids (figure 1d), second-order optic neuropil abuts the lateral edge of the arcuate body. In solfugids (figure 1d) a bundle of axons leaves the lenticular second optic neuropil in the brain and reaches the arcuate body's flank. In araneans, the second optic neuropil of each principal eye supplies a major projection to this midline neuropil (Hanström 1926; Strausfeld et al. 1993). Studies of the spider C. salei (Strausfeld et al. 1993) show that visual relay neurons project tangentially across perpendicular neurons of the arcuate body without any evidence of retinotopic mapping. This suggests that columnar organization of the arcuate body is independent of the development of the eye and peripheral optic neuropils and has no affinity with the columnar organization of mandibulate optic lobe neuropils. The relationship between visual neuropils and arcuate midline neuropil thus distinguishes the chelicerate and onychophoran visual systems from those of tetraconates and scutigerid chilopods, in which compound eyes supply nested retinotopic neuropils in discrete optic lobes (Strausfeld 2005).

Figure 2
Comparison of Arctonoe fragilis and examples of Mandibulata, with reference to midline neuropils. (a) Silver-stained brain of A. fragilis, showing the paired mushroom bodies (MB) and olfactory lobes (Olf Lo). Neuropils are bilaterally elaborated but there ...
Figure 3
Arcuate midline neuropils shared by (a) onychophorans and (b–h) chelicerate taxa ((b) Limulus polyphemus (Xiphosura); (c) Centruroides sp. (Scorpionida); (d) Eremobates pallipes (Solfugida); (e) Heptathela kimurai (Aranaea); (f) Pardosa sp. (wolf ...
Figure 4
Homologous stratification in midline neuropils of (a, d, e) E. rowelli, the spiders (b, c) Delana cancerides and (f) Pardosa sp. (a) Anti-5HT staining of E. rowelli reveals a diffusely packed immunoreactive stratum (1) that is distinct from a palisade ...

Silver staining and immunocytology (Loesel et al. 2002) reveal that the neuroarchitecture of the arcuate body in E. rowelli (figures 3a and and44a,d,e) is similar in detail to that of the chelicerate arcuate body (figures 3bh and 4b,c,f). The midline neuropil of E. rowelli (figure 4e) and the arcuate body of the New World hunting spider Pardosa sp. (figure 4f) both demonstrate successive strata of looped axons and tangential fibres that are intersected by ensembles of perpendicular axons. Immunostaining against allatostatin and serotonin reveals the same sequence of stratification in the midline neuropils of both Onychophora (figure 4a,d) and the aranean D. cancerides (figure 4b,c). These arrangements are characteristic of the Onychophora and chelicerates but not of other arthropod groups. That so many identical arrangements might be convergent homoplasies is highly unlikely, leading us to favour the view that arcuate body architectures are synapomorphies uniting the Onychophora and chelicerates. As is discussed below, the possibility that arcuate bodies might be plesiomorphic modifications of a midline neuropil ground pattern pertaining to all arthropods is argued as unlikely due to fundamental differences in the cellular identities of modules that make up midline neuropils in mandibulates and chelicerates.

(c) Phylogenetic relationships implied by brain organization

Phylogenetic relationships were examined using PAUP (see §2). Trees were computed as unrooted (figure 5a,b). Bootstrap values from 1000 replicates are shown in figure 5b. Neighbour joining and unweighted pair group method with arithmetic mean (UPGMA) showed congruence. No islands of trees were detected, indicating little conflict of signal in the data. Of the eight best trees (255 steps, CI=0.455) all resolve a monophyletic Chelicerate clade, which includes the Pycnogonidae and a sister-group relationship for the chelicerates and onychophorans. The latter has strong bootstrap support (90%). Enforcing polyphyly for this clade results in 17 shortest trees, each nine steps longer than the three best trees (264 steps, CI=0.439). Four independent characters (generally uniform cerebral somata, midline neuropils provided by medially positioned somata, midline neuropil superficial to protocerebrum, visual supply to the lateral margin of medial neuropil) and one suite of characters (stratification within midline neuropil) are synapomorphic for this group.

(d) Reliability of neuronal structures for phylogenetic analysis

Traditional use of morphological criteria to reconstruct phylogenies has focused on external features, such as limbs, mouthparts and segmental specializations. Such potentially adaptive characters can be difficult to interpret as a result of convergence, parallelism and other such processes. Further, homologous structures can vary greatly even among species recognized as related, as attested by gene expression showing the gnathobasic nature of mandibles in insects and crustaceans (Popadic et al. 1998). Nevertheless, that structural studies indeed provide reliable indicators for phylogenetic inference is evidenced by numerous analyses of fossil material that contribute to the resolution of crustacean stem lineages (see Walossek & Müller 1998), and many morphological features have been shown to unite taxa or provide crucial characters for phylogeny (Edgecombe et al. 2002; Giribet et al. 2002). But how reliable are neuroanatomical structures for inferring phylogenies? Many neurological features are indisputably consistent across taxa and reveal plesiomorphies. For example, uncrossed retinotopic axons linking the deepest optic neuropil with the next most peripheral is a ground plan shared across the Tetraconata (Strausfeld 2005), supporting an origin of the hexapods from basal entomostracans. The presence of neuroblasts and a similar axonogenesis of pioneer neurons in insects and crustaceans, which show fundamental differences from chilopods (Whitington et al. 1991; Whitington 2004; Harzsch 2003), or the expression of the transcription factor gene Even-skipped in identical neurons across insects and crustaceans (Duman-Scheel & Patel 1999) also unite the assemblage Entomostraca+Hexapoda+Malacostraca.

The structure of neuropils is generally highly conserved within any crustacean or insect order (Sandeman & Scholtz 1995; Farris 2005). The following are just a few examples. Paired noduli of the central complex unite pterygote insects (Loesel et al. 2002). Male-specific antennal lobe glomeruli and grooved lobules in the optic lobes are uniquely lepidopteran synapomorphies. The spacing of columns in the third optic neuropil of eumalacostracans represents each facet of the compound eye, whereas in insects assemblies of retinotopic neurons in the homologous neuropil coarsen the retinotopic mosaic (Sinakevitch et al. 2003). In mushroom bodies, centres thought to be concerned with learning and memory and therefore subject to strong selection pressure, the same morphological classes of intrinsic neurons differentiate in a characteristic sequence, but the size, layering and disposition of the calyces and lobes assume features that are characteristic of each neopteran order (Farris & Sinakevitch 2003).

Analysis of brain structure reveals a suite of neuroarchitectural arrangements shared between onychophorans and chelicerates that strongly support an affinity of these two groups. In scorpions, solfugids, araneans and onychophorans, heterolateral lobes connect the two mushroom bodies, a situation unknown in other arthropods other than broad complex mutants of Drosophila where one division of the mushroom bodies' medial lobes crosses the brain midline (Michel et al. 2004). The columnar and multi-stratified architectures and their immunoaffinities in the arcuate bodies of onychophorans correspond to those of araneans (figure 4). In addition to the many striking similarities between the supraoesophageal neuropils of onychophorans and chelicerates, the post-stomodeal ganglia of onychophorans are unfused at the midline, with the two halves linked by numerous connectives, as is typical of juvenile xiphosurans (Mittman & Scholtz 2003). The feature of uniformly small neuronal perikarya is another characteristic of chelicerate and onychophoran brains that is not shared by the brains of Chilopoda and Tetraconata. Finally, a unique type of synaptic complex is common to a specific region of the onychophoran and opilionid mushroom bodies (figure 1eg). Synaptic configurations as phylogenetically diagnostic elements have a precedent, as in the case of the T-shaped presynaptic active zone that is diagnostic of the Diptera (Shaw & Meinertzhagen 1986).

4. Evolutionary implications

The present neuromorphology-based cladogram (figure 5a,b; see also electronic supplementary materials) generally agrees with published analyses based on RNA and DNA data with regard to relationships within the Tetraconata and within the chelicerates sensu stricto (Ballard et al. 1992; Friedrich & Tautz 1995; Zrzavy et al. 1998). The exception is the position of the Onychophora, reminiscent of other ambiguous placements ranging from the Onychophora being nested within the ‘pancrustaceans’ (Peterson & Eernisse 2001), through their comprising (with the tardigrades) a sister group of the arthropods (Giribet et al. 2000; Regier et al. 2005), to their representing a lobopod stem taxon (Budd 1996). Both neuroanatomical observations and parsimony analysis suggest that this group should be integrated within the Euarthropoda as an ancient sister group of the Chelicerata.

This proposal conflicts with the received opinion that the Onychophora are not incorporated within the euarthropods but are a sister group. Supporting evidence includes the observation that onychophoran muscle possesses filament-like components similar to intermediate filaments of deuterostomes and lophotrochozoans (Bartnik & Weber 1989). The evolution of the haemocyanin superfamily (Immesberger & Burmester 2004) demonstrates that onychophoran respiratory haemocyanins were the first to diverge from ancestral phenoloxidases thus suggesting that this group may be distinct from other arthropods. Onychophorans also retain other plesiomorphic features such as the lack of sclerotization, a sub-epidermal muscle layer and ciliated coelomoducts. A combined phylogenetic analysis, using ribosomal and nuclear protein coding genes, as well as mitochondrial genes, also places the onychophorans as a clade distinct from the Euarthropoda (Giribet et al. 2001), a conclusion similarly reached by Regier et al. (2005), by Mallatt et al. (2004) using 28S and 18S rRNA gene sequences and by Edgecombe et al. (2000) from histone H3 and U2 snRNA sequences.

On the other hand, a study based on 12S ribosomal RNA sequences (Ballard et al. 1992) includes Onychophora within the Euarthropoda, placing them as a sister group to the chelicerates, as does the present analysis using neural characters. The question remains whether neuroanatomical features here are sufficient to propose that the Onychophora are related to the chelicerates despite the many primitive features of the onychophoran body plan, including the paired protocerebral antennae, recently shown to be modified legs (Mayer & Koch 2005).

Brain anatomy suggests that if Onychophora are a sister group of the chelicerates, they probably represent an early branch of the chelicerate evolutionary trajectory. This would imply that cerebral organization characteristic of Onychophora has been essentially maintained throughout subsequent chelicerate evolution. Indeed, if there was no such relationship between the Chelicerata and Onychophora, then the similarities described in this account would demand an exceptional degree of homoplasy and, therefore, ‘parallel evolution’.

But is the striking similarity between onychophoran and chelicerate arcuate bodies merely a plesiomorphic modification of a midline ground plan common to all arthropods? That this is unlikely is suggested by comparisons across mandibulates (figure 2) and chelicerates (figure 3) demonstrating fundamental differences of midline architectures and their contributing neurons (table 1). In mandibulates, midline neuropils are embedded within the protocerebral matrix and derived from heterolateral processes originating from a small population of remotely located somata (figure 2cj). Elaboration at the midline is provided by heterolateral neurons, the collaterals of which supply repeat units or ‘modules’ with a subsequent recruitment of perpendicular (often peptidergic) neurons. This arrangement is simplest in basal scutigerid chilopods (figure 2c), more elaborate in basal crustaceans (figure 2e) and most elaborate in pterygote insects (figure 2j). An organization that might represent an early stage of the evolution of mandibulate midline neuropils may have been retained in the Symphyla where some heterolateral neurons reveal subtle differences of branching at the midline (figure 2h).

In chelicerates and onychophorans, the midline neuropils are always located superficial to the rest of the protocerebrum and they derive from thousands of local somata at the midline (figure 3bh). These provide the arcuate body's modular organization and also contribute to its tangential organization.

An ancestral character state that would reconcile differences between mandibulate and chelicerate/onychophoran midline architectures is a simple rectilinear network comprising heterolateral neurons intersected by perpendicular elements (figure 2k). Such a rectilinear network, exemplified by the post-stomodeal ladder-like ‘Markstrang’ of certain annelids (Orrhage & Müller 2005), is likely to have existed as a ring-like structure around a mouth long before the origin of segmented invertebrates. An implication of this is that divergence of two distinct midline ground patterns is likely to have occurred very early in arthropod evolution.

In conclusion, we propose that the brains of onychophorans represent an archaic pattern of organization that has been maintained in chelicerates and which differs from the organization of mandibulate brains. That diagnostic features such as the arcuate bodies might be plesiomorphic characters of a basal arthropod ground pattern is unlikely considering the substantial differences between the arcuate bodies of chelicerates and onychophorans and the midline neuropils of mandibulates.

Acknowledgments

This study was supported by a fellowship from the John D. and Catherine T. McArthur foundation to N.J.S. We thank two anonymous referees for their suggestions, and also the following: Drs A. D. Blest and J. Zeil at the RSBS, Australian National University, Canberra; Dr Penny Greenslade, CSIRO, Canberra; Prof. Yoshihiro Toh, Kyushu University and the Japan Society for the Promotion of Science; the staff of the University of Washington's Friday Harbor Marine Biology Laboratory; Prof. M. Heisenberg and Dr P. Masek, University of Würzburg; and the Alexander von Humboldt Foundation.

Supplementary Material

Matrix: character/taxa matrix used for this analysis:

Descriptions of the 112 characters used.

Character list and description:

Characters are treated as unlinked, unordered, equally weighted, and either present or absent. Characters are not assigned character states. All characters are structural entities without functional affiliations.

References

  • Aguinaldo A.M, Turbeville J.M, Linford L.S, Rivera M.C, Garey J.R, Raff R.A, Lake J.A. Evidence for a clade of nematodes, arthropods and other moulting animals. Nature. 1997;387:489–493. 10.1038/387489a0 [PubMed]
  • Ballard J.W.O, Olsen G.J, Faith D.P, Odgers W.A, Rowell D.M, Atkinson P.W. Evidence from 12S ribosomal RNA sequences that onychophorans are modified arthropods. Science. 1992;258:1345–1348. [PubMed]
  • Bartnik E, Weber K. Widespread occurrence of intermediate filaments in invertebrates: common principles and aspects of diversion. Eur. J. Cell Biol. 1989;50:17–33.
  • Budd G.E. The morphology of Opabinia regalis and the reconstruction of the arthropod stem group. Lethaia. 1996;29:1–14.
  • Colgan D.J, McLaughlan A, Wilson G.D.F, Livingston S.P, Edgecomb G.D, Macaranas J, Cassis G, Gray M.R. Histone H3 and U2 snRNA DNA sequences and arthropod molecular evolution. Aust. J. Zool. 1998;46:419–437. 10.1071/ZO98048
  • Dohle W. Are the insects terrestrial crustaceans? A discussion of some new facts and arguments and the proposal of the proper name ‘Tetraconata’ for the monophyletic unit Crustacea+Hexapoda. Ann. Soc. Entomol. France. 2001;37:85–103.
  • Duman-Scheel M, Patel N.H. Analysis of molecular marker expression reveals neuronal homology in distantly related arthropods. Development. 1999;126:2327–2334. [PubMed]
  • Edgecombe G.D, Wilson G.D.F, Colgan D.J, Gray M.R, Cassis G. Arthropod cladistics, combined analysis of histone H3 and U2 snRNA sequences and morphology. Cladistics. 2000;16:155–203. 10.1111/j.1096-0031.2000.tb00352.x
  • Edgecombe G.D, Giribet G, Wheeler W.W. Phylogeny of Henicopidae (Chilopoda, Lithobiomorpha): a combined analysis of morphology and five molecular loci. Syst. Entomol. 2002;27:31–64. 10.1046/j.0307-6970.2001.00163.x
  • Eriksson B.J, Tait N.N, Budd G.E. Head development in the onychophoran Euperipatoides kanangrensis with particular reference to the central nervous system. J. Morphol. 2003;255:1–23. 10.1002/jmor.10034 [PubMed]
  • Fahrenbach W.H, Chamberlain S.C. The brain of the horseshoe crab Limulus polyphemus. In: Gupta A.P, editor. Arthropod brain: its evolution, structure and functions. Wiley; New York, NY: 1987. pp. 63–94.
  • Fanenbruck M, Harzsch S. A brain atlas of Godzilliognomus frondosus Yager, 1989 (Remipedia, Godzilliidae) and comparison with the brain of Speleonectes tulumensis Yager, 1987 (Remipedia, Speleonectidae): implications for arthropod relationships. Arthropod Struct. Dev. 2005;34:343–378. 10.1016/j.asd.2005.01.007
  • Farris S.M. Evolution of insect mushroom bodies: old clues, new insights. Arthropod Struct. Dev. 2005;34:211–234. 10.1016/j.asd.2005.01.008
  • Farris S.M, Sinakevitch I. Development and evolution of the insect mushroom bodies: towards the understanding of conserved developmental mechanisms in a higher brain center. Arthropod Struct. Dev. 2003;32:79–101. [PubMed]
  • Flögel J.H.L. Über den feineren Bau des Arthropodengehirns. Tagbl. Versamml. dtschr. Naturforsch. Ärzte. (Beilage) 1876;49:115–120.
  • Friedrich M, Tautz D. Ribosomal DNA phylogeny of the major extant arthropod classes and the evolution of the myriapods. Nature. 1995;376:165–167. 10.1038/376165a0 [PubMed]
  • Giribet G, Distel D.L, Polz M, Sterrer W, Wheeler W.C. Triploblastic relationships with emphasis on the acoelomates and the position of Gnathostomulida, Cycliophora, Plathelminthes, and Chaetognatha: a combined approach of 18S rDNA sequences and morphology. Syst. Biol. 2000;49:539–562. 10.1080/10635159950127385 [PubMed]
  • Giribet G, Edgecombe G, Wheeler W.C. Arthropod phylogeny based on eight molecular loci and morphology. Nature. 2001;413:157–161. 10.1038/35093097 [PubMed]
  • Giribet G, Edgecombe G.D, Wheeler W.W, Babbit C. Phylogeny and systematic position of Opiliones: a combined analysis of chelicerate relationships using morphological and molecular data. Cladistics. 2002;18:5–70. [PubMed]
  • Grenier J.K, Garber T.L, Warren R, Whitington P.M, Carroll S. Evolution of the entire arthropod Hox gene set predated the origin and radiation of the onychophoran/arthropod clade. Curr. Biol. 1997;7:547–553. 10.1016/S0960-9822(06)00253-3 [PubMed]
  • Hanström, B. 1926 Vergleichende Anatomie des Nervensystems der wirbellosen Tiere. (Facsimile reprint by A. Asher, Amsterdam 1968.)
  • Harzsch S. Ontogeny of the ventral cord in malacostracan crustaceans: a common plan for neuronal development in Crustacea, Hexapoda and other Arthropoda. Arthropod Struct. Dev. 2003;32:17–38. 10.1016/S1467-8039(03)00008-2 [PubMed]
  • Holmgren N. Zur vergleichenden Anatomie des Gehirns von Polychaeten, Onychophoren, Xiphosuren, Arachniden, Crustaceen, Myriapoden, und Insekten. K. Svenska Vetensk. Akad. Handl. 1916;56:1–200.
  • Hou X, Bergström J. Cambrian lobopodians—ancestors of extant onychophorans? Zool. J. Linn. Soc. 1995;114:3–34. 10.1006/zjls.1995.0014
  • Immesberger A, Burmester T. Putative phenoloxidases in the tunicate Ciona intestinalis and the origin of the arthropod hemocyanin superfamily. J. Comp. Physiol. B. 2004;174:169–180. 10.1007/s00360-003-0402-4 [PubMed]
  • Kenyon F.C. The meaning and structure of the so-called “mushroom bodies” of the hexapod brain. Am. Nat. 1896;30:643–650. 10.1086/276450
  • Kusche K, Ruhberg H, Burmeister T. A hemocyanin from the Onychophora and the emergence of respiratory proteins. Proc. Natl Acad. Sci. USA. 2002;99:10 545–10 548. 10.1073/pnas.152241199 [PMC free article] [PubMed]
  • Loesel R, Nässel D.R, Strausfeld N.J. Common design in a unique mid-line neuropil in the brains of arthropods. Arthropod Struct. Dev. 2002;31:77–91. 10.1016/S1467-8039(02)00017-8 [PubMed]
  • McKinzie M.E, Benton J.L, Beltz B.S, Mellon D. Parasol cells of the hemiellipsoid body in the crayfish Procambarus clarkii: dendritic branching patterns and functional implications. J. Comp. Neurol. 2003;462:168–179. 10.1002/cne.10716 [PubMed]
  • Mallatt J.M, Garey J.R, Shultz J.W. Ecdysozoan phylogeny and Bayesian inference: first use of nearly complete 28S and 18S rRNA gene sequences to classify the arthropods and their kin. Mol. Phylogenet. Evol. 2004;31:178–191. 10.1016/j.ympev.2003.07.013 [PubMed]
  • Mayer G, Koch M. Ultrastructure and fate of the nephridial Anlagen in the antennal segment of Epiperipatus biolleyi (Onychophora, Peripatidae)—evidence for the onychophoran antennae being modified legs. Arthropod Struct. Dev. 2005;34:471–480. 10.1016/j.asd.2005.03.004
  • Melzer R.R, Michalke M, Smola U. Walking on insect paths? Early ommatidial development in the compound eye of the ancestral crustacean, Triops cancriformis. Naturwissenschaften. 2000;87:308–311. 10.1007/s001140050727 [PubMed]
  • Michel C.I, Kraft R, Restifo L.L. Defective neuronal development in the mushroom bodies of Drosophila fragile X mental retardation 1 mutants. J. Neurosci. 2004;24:5798–5809. 10.1523/JNEUROSCI.1102-04.2004 [PubMed]
  • Mittmann B, Scholtz G. Development of the nervous system in the “head” of Limulus polyphemus (Chelicerata: Xiphosura): morphological evidence for a correspondence between the segments of the chelicerae and of the (first) antennae of Mandibulata. Dev. Genes Evol. 2003;213:9–17. [PubMed]
  • Orrhage L, Müller M.C.M. Morphology of the nervous system of Polychaeta (Annelida) Hydrobiologia. 2005;535–536:79–111.
  • Peterson K.J, Eernisse D.J. Animal phylogeny and the ancestry of bilaterians: inferences from morphology and 18S rDNA gene sequences. Evol. Dev. 2001;3:170–205. 10.1046/j.1525-142x.2001.003003170.x [PubMed]
  • Popadic A, Panganiban G, Rusch D, Shear W.A, Kaufman T.C. Molecular evidence for the gnathobasic derivation of arthropod mandibles and for the appendicular origin of the labrum and other structures. Dev. Genes Evol. 1998;208:142–150. 10.1007/s004270050165 [PubMed]
  • Ramsköld L, Chen J. Cambrian lobopodians: morphology and phylogeny. In: Edgecombe G.D, editor. Arthropod fossils and phylogeny. Columbia University Press; New York, NY: 1998. pp. 107–150.
  • Regier J.C, Shulz J.W, Kambic R.E. Pancrustacean phylogeny: hexapods are terrestrial crustaceans and maxillopods are not monophyletic. Proc. R. Soc. B. 2005;272:395–401. 10.1098/rspb.2004.2917 [PMC free article] [PubMed]
  • Richter S. The Tetraconata concept: hexapod–crustacean relationships and the phylogeny of Crustacea. Org. Divers. Evol. 2002;2:217–237. 10.1078/1439-6092-00048
  • Sandeman D, Scholtz G. Ground plans, evolutionary changes and homologies in decapod crustacean brains. In: Breidbach O, Kutsch W, editors. The nervous system of invertebrates: an evolutionary and comparative approach. Birkhäuser; Basel, Switzerland: 1995. pp. 329–347.
  • Schürmann F.W. Histology and ultrastructure of the onychophoran brain. In: Gupta A.P, editor. Arthropod brain: its evolution, structure and functions. Wiley; New York, NY: 1987. pp. 159–180.
  • Shaw S.R, Meinertzhagen I.A. Evolutionary progression at synaptic connections made by identified homologous neurones. Proc. Natl Acad. Sci. USA. 1986;83:7961–7965. [PMC free article] [PubMed]
  • Sinakevitch I, Douglass J.K, Scholtz G, Loesel R, Strausfeld N.J. Conserved and convergent organisation in the optic lobes of insects and isopods, with reference to other crustacean taxa. J. Comp. Neurol. 2003;467:150–172. 10.1002/cne.10925 [PubMed]
  • Strausfeld N.J. Crustacean–insect relationships, the use of brain characters to derive phylogeny amongst segmented invertebrates. Brain Behav. Evol. 1998;52:186–206. 10.1159/000006563 [PubMed]
  • Strausfeld N.J. The evolution of crustacean and insect optic lobes and the origins of chiasmata. Arthropod Struct. Dev. 2005;34:235–256. 10.1016/j.asd.2005.04.001
  • Strausfeld N.J, Barth F.G. Two visual systems in one brain: neuropils serving the secondary eyes of the spider Cupiennius salei. J. Comp. Neurol. 1993;328:43–62. 10.1002/cne.903280104 [PubMed]
  • Strausfeld N.J, Li Y. Representation of the calyces in the medial and vertical lobes of the cockroach mushroom bodies. J. Comp. Neurol. 1999;409:626–646. 10.1002/(SICI)1096-9861(19990712)409:4%3C626::AID-CNE8%3E3.0.CO;2-B [PubMed]
  • Strausfeld N.J, Weltzien P, Barth F.G. Two visual systems in one brain: neuropils serving the principal eyes of the spider Cupiennius salei. J. Comp. Neurol. 1993;328:63–75. 10.1002/cne.903280105 [PubMed]
  • Strausfeld N.J, Buschbeck E.K, Gomez R.S. The arthropod mushroom body, its roles, evolutionary enigmas and mistaken identities. In: Breidbach O, Kutsch W, editors. The nervous system of invertebrates, an evolutionary and comparative approach. Birkhäuser; Basel, Switzerland: 1995. pp. 349–381.
  • Strausfeld N.J, Hansen L, Li Y.-S, Gomez R.S, Ito K. Evolution, discovery, and interpretations of arthropod mushroom bodies. Learn. Mem. 1998;5:11–37. [PMC free article] [PubMed]
  • Stys P, Zrzavy J. Phylogeny and classification of extant Arthropoda: review of hypotheses and nomenclature. Eur. J. Entomol. 1994;91:257–275.
  • Swofford D.L. PAUP*: phylogenetic analysis using parsimony (*and other methods), v. 4.0.b10. Sinauer Associates Inc; Sunderland, MA: 2002.
  • Walossek D, Müller K.J. Early arthropod phylogeny in the light of the Cambrian ‘Orsten’ fossils. In: Edgecombe G.D, editor. Arthropod fossils and phylogeny. Columbia University Press; New York, NY: 1998. pp. 185–231.
  • Waloszek D, Chen J, Maas A, Wang X. Early Cambrian arthropods—new insights into arthropod head and structural evolution. Arthropod Struct. Dev. 2005;34:189–205. 10.1016/j.asd.2005.01.005
  • Whitington P.M. The development of the crustacean nervous system. In: Scholtz G, editor. Evolutionary developmental biology of crustacea. Crustacean issues. vol. 15. A. A. Balkema; The Netherlands: 2004. pp. 135–167.
  • Whitington P.M, Meier T, King P. Segmentation, neurogenesis and formation of early axonal pathways in the centipede, Erostigmus rubripes (Brandt) Wilhelm Roux's Arch. Dev. Biol. 1991;199:349–363.
  • Zrzavy J, Mihulka S, Kepka P, Bezdek A, Tietz D. Phylogeny of the metazoa based on morphological and 18S ribosomal DNA evidence. Cladistics. 1998;14:249–285. 10.1111/j.1096-0031.1998.tb00338.x

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