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Antimicrob Agents Chemother. 1996 Feb; 40(2): 477–480.
PMCID: PMC163139

Mutations in 23S rRNA are associated with clarithromycin resistance in Helicobacter pylori.

Abstract

Twelve clarithromycin-resistant Helicobacter pylori isolates (100% of resistant isolates examined) from seven different patients each contained an A-->G transition mutation within a conserved loop of 23S rRNA. A-->G transition mutations at positions cognate with Escherichia coli 23S rRNA positions 2058 and 2059 were identified. Clarithromycin-susceptible H. pylori isolates from 14 different patients displayed no polymorphisms in a conserved loop within domain V of 23S rRNA. The study is the first to report mutations in H. pylori associated with resistance to an antimicrobial agent used in established peptic ulcer treatment regimens.

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Selected References

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  • Bayerdörffer E, Neubauer A, Rudolph B, Thiede C, Lehn N, Eidt S, Stolte M. Regression of primary gastric lymphoma of mucosa-associated lymphoid tissue type after cure of Helicobacter pylori infection. MALT Lymphoma Study Group. Lancet. 1995 Jun 24;345(8965):1591–1594. [PubMed]
  • Bukanov NO, Berg DE. Ordered cosmid library and high-resolution physical-genetic map of Helicobacter pylori strain NCTC11638. Mol Microbiol. 1994 Feb;11(3):509–523. [PubMed]
  • Cseplö A, Etzold T, Schell J, Schreier PH. Point mutations in the 23 S rRNA genes of four lincomycin resistant Nicotiana plumbaginifolia mutants could provide new selectable markers for chloroplast transformation. Mol Gen Genet. 1988 Oct;214(2):295–299. [PubMed]
  • Ettayebi M, Prasad SM, Morgan EA. Chloramphenicol-erythromycin resistance mutations in a 23S rRNA gene of Escherichia coli. J Bacteriol. 1985 May;162(2):551–557. [PMC free article] [PubMed]
  • Go MF, Chan KY, Versalovic J, Koeuth T, Graham DY, Lupski JR. Cluster analysis of Helicobacter pylori genomic DNA fingerprints suggests gastroduodenal disease-specific associations. Scand J Gastroenterol. 1995 Jul;30(7):640–646. [PubMed]
  • Goldman RC, Zakula D, Flamm R, Beyer J, Capobianco J. Tight binding of clarithromycin, its 14-(R)-hydroxy metabolite, and erythromycin to Helicobacter pylori ribosomes. Antimicrob Agents Chemother. 1994 Jul;38(7):1496–1500. [PMC free article] [PubMed]
  • Graham DY, Lew GM, Klein PD, Evans DG, Evans DJ, Jr, Saeed ZA, Malaty HM. Effect of treatment of Helicobacter pylori infection on the long-term recurrence of gastric or duodenal ulcer. A randomized, controlled study. Ann Intern Med. 1992 May 1;116(9):705–708. [PubMed]
  • Hardy DJ, Hanson CW, Hensey DM, Beyer JM, Fernandes PB. Susceptibility of Campylobacter pylori to macrolides and fluoroquinolones. J Antimicrob Chemother. 1988 Nov;22(5):631–636. [PubMed]
  • Labenz J, Börsch G. Highly significant change of the clinical course of relapsing and complicated peptic ulcer disease after cure of Helicobacter pylori infection. Am J Gastroenterol. 1994 Oct;89(10):1785–1788. [PubMed]
  • Logan RP, Gummett PA, Schaufelberger HD, Greaves RR, Mendelson GM, Walker MM, Thomas PH, Baron JH, Misiewicz JJ. Eradication of Helicobacter pylori with clarithromycin and omeprazole. Gut. 1994 Mar;35(3):323–326. [PMC free article] [PubMed]
  • Marshall BJ. Helicobacter pylori. Am J Gastroenterol. 1994 Aug;89(8 Suppl):S116–S128. [PubMed]
  • Meier A, Kirschner P, Springer B, Steingrube VA, Brown BA, Wallace RJ, Jr, Böttger EC. Identification of mutations in 23S rRNA gene of clarithromycin-resistant Mycobacterium intracellulare. Antimicrob Agents Chemother. 1994 Feb;38(2):381–384. [PMC free article] [PubMed]
  • Menninger JR. Functional consequences of binding macrolides to ribosomes. J Antimicrob Chemother. 1985 Jul;16 (Suppl A):23–34. [PubMed]
  • Moazed D, Noller HF. Chloramphenicol, erythromycin, carbomycin and vernamycin B protect overlapping sites in the peptidyl transferase region of 23S ribosomal RNA. Biochimie. 1987 Aug;69(8):879–884. [PubMed]
  • Skinner R, Cundliffe E, Schmidt FJ. Site of action of a ribosomal RNA methylase responsible for resistance to erythromycin and other antibiotics. J Biol Chem. 1983 Oct 25;258(20):12702–12706. [PubMed]
  • Sor F, Fukuhara H. Identification of two erythromycin resistance mutations in the mitochondrial gene coding for the large ribosomal RNA in yeast. Nucleic Acids Res. 1982 Nov 11;10(21):6571–6577. [PMC free article] [PubMed]
  • Stolte M, Eidt S. Healing gastric MALT lymphomas by eradicating H pylori? Lancet. 1993 Sep 4;342(8871):568–568. [PubMed]
  • Sung JJ, Chung SC, Ling TK, Yung MY, Leung VK, Ng EK, Li MK, Cheng AF, Li AK. Antibacterial treatment of gastric ulcers associated with Helicobacter pylori. N Engl J Med. 1995 Jan 19;332(3):139–142. [PubMed]
  • Vester B, Garrett RA. A plasmid-coded and site-directed mutation in Escherichia coli 23S RNA that confers resistance to erythromycin: implications for the mechanism of action of erythromycin. Biochimie. 1987 Aug;69(8):891–900. [PubMed]
  • Weisblum B. Erythromycin resistance by ribosome modification. Antimicrob Agents Chemother. 1995 Mar;39(3):577–585. [PMC free article] [PubMed]
  • Wotherspoon AC, Doglioni C, Diss TC, Pan L, Moschini A, de Boni M, Isaacson PG. Regression of primary low-grade B-cell gastric lymphoma of mucosa-associated lymphoid tissue type after eradication of Helicobacter pylori. Lancet. 1993 Sep 4;342(8871):575–577. [PubMed]

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