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Plant Physiol. 1996 Aug; 111(4): 1321–1328.
PMCID: PMC161017

Differential regulation of trichome formation on the adaxial and abaxial leaf surfaces by gibberellins and photoperiod in Arabidopsis thaliana (L.) Heynh.


In wild-type (WT) Columbia and Landsberg erecta ecotypes of Arabidopsis thaliana (L.) Heynh., trichomes are present on the adaxial surfaces of all rosette leaves but are absent from the abaxial surfaces of the first-formed leaves. We have determined that both long-day (LD) photoperiod and gibberellin (GA) stimulate trichome formation. WT plants grown in LD conditions produce the first abaxial trichome on earlier leaves than plants grown in short-day (SD) conditions. Photoperiod sensitivity of abaxial trichome formation on WT plants develops gradually over time, reaching the maximum sensitivity about 24 d after germination. Application of gibberellic acid to WT plants growing in SD conditions accelerates the onset of abaxial trichomes. Conversely, application of 20 to 80 mg L-1 paclobutrazol, a GA biosynthesis inhibitor, to wild-type plants suppresses trichome initiation on the abaxial epidermis. The GA-deficient mutants ga1-5 and ga4-1 and the GA-insensitive mutant gai-1 exhibit delayed onset of abaxial trichomes when grown in LD conditions. The null mutant ga1-3 produces completely glabrous leaves when grown in SD conditions. Application of gibberellic acid to glabrous ga1-3 plants consistently induces earlier formation of trichomes on the adaxial epidermis than on the abaxial epidermis, demonstrating a difference between the adaxial and abaxial surfaces in their response to GA with regard to trichome formation.

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Selected References

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  • Chiang HH, Hwang I, Goodman HM. Isolation of the Arabidopsis GA4 locus. Plant Cell. 1995 Feb;7(2):195–201. [PMC free article] [PubMed]
  • Evans MM, Poethig RS. Gibberellins promote vegetative phase change and reproductive maturity in maize. Plant Physiol. 1995 Jun;108(2):475–487. [PMC free article] [PubMed]
  • Gubler F, Kalla R, Roberts JK, Jacobsen JV. Gibberellin-regulated expression of a myb gene in barley aleurone cells: evidence for Myb transactivation of a high-pI alpha-amylase gene promoter. Plant Cell. 1995 Nov;7(11):1879–1891. [PMC free article] [PubMed]
  • Hülskamp M, Misŕa S, Jürgens G. Genetic dissection of trichome cell development in Arabidopsis. Cell. 1994 Feb 11;76(3):555–566. [PubMed]
  • Koornneef M, Hanhart CJ, van der Veen JH. A genetic and physiological analysis of late flowering mutants in Arabidopsis thaliana. Mol Gen Genet. 1991 Sep;229(1):57–66. [PubMed]
  • Lloyd AM, Walbot V, Davis RW. Arabidopsis and Nicotiana anthocyanin production activated by maize regulators R and C1. Science. 1992 Dec 11;258(5089):1773–1775. [PubMed]
  • Meinke DW, Franzmann LH, Nickle TC, Yeung EC. Leafy Cotyledon Mutants of Arabidopsis. Plant Cell. 1994 Aug;6(8):1049–1064. [PMC free article] [PubMed]
  • Metzger JD. Role of Gibberellins in the Environmental Control of Stem Growth in Thlaspi arvense L. Plant Physiol. 1985 May;78(1):8–13. [PMC free article] [PubMed]
  • Oppenheimer DG, Herman PL, Sivakumaran S, Esch J, Marks MD. A myb gene required for leaf trichome differentiation in Arabidopsis is expressed in stipules. Cell. 1991 Nov 1;67(3):483–493. [PubMed]
  • Sun TP, Kamiya Y. The Arabidopsis GA1 locus encodes the cyclase ent-kaurene synthetase A of gibberellin biosynthesis. Plant Cell. 1994 Oct;6(10):1509–1518. [PMC free article] [PubMed]
  • Wilson RN, Heckman JW, Somerville CR. Gibberellin Is Required for Flowering in Arabidopsis thaliana under Short Days. Plant Physiol. 1992 Sep;100(1):403–408. [PMC free article] [PubMed]
  • Zeevaart JA, Gage DA. ent-kaurene biosynthesis is enhanced by long photoperiods in the long-day plants Spinacia oleracea L. and Agrostemma githago L. Plant Physiol. 1993 Jan;101(1):25–29. [PMC free article] [PubMed]

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