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Plant Cell. Jan 1995; 7(1): 43–55.
PMCID: PMC160763

Transient induction of a peroxidase gene in Medicago truncatula precedes infection by Rhizobium meliloti.


Although key determinative events of the Rhizobium-legume symbiosis are likely to precede bacterial infection, no plant genes have been identified that are expressed strongly prior to infection and nodule morphogenesis. A subtractive hybridization-polymerase chain reaction technique was used to enrich for genes induced during the early phases of the R. meliloti-Medicago truncatula symbiosis. One gene so identified encodes a putative plant peroxidase protein, which we have named Rip1 for Rhizobium-induced peroxidase. The accumulation of rip1 transcript was rapidly and transiently induced by R. meliloti and by the corresponding lipooligosaccharide signal molecule Nod factor RmIV, which was both necessary and sufficient for rip1 induction. The duration of maximal rip1 expression coincided with the preinfection period: transcript levels for rip1 were near maximal by 3 hr postinoculation and declined by 48 hr, coincident with early infection events and the onset of nodule morphogenesis. Furthermore, although rip1 induction preceded bacterial infection by at least 24 hr, the transcript was localized to epidermal cells in the differentiating root zone that was subsequently infected by Rhizobium. Thus, a defining feature of the Rhizobium infection court is the prior induction of rip1 expression.

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Selected References

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  • Apostol I, Heinstein PF, Low PS. Rapid Stimulation of an Oxidative Burst during Elicitation of Cultured Plant Cells : Role in Defense and Signal Transduction. Plant Physiol. 1989 May;90(1):109–116. [PMC free article] [PubMed]
  • Bhuvaneswari TV, Bhagwat AA, Bauer WD. Transient susceptibility of root cells in four common legumes to nodulation by rhizobia. Plant Physiol. 1981 Nov;68(5):1144–1149. [PMC free article] [PubMed]
  • Bradley DJ, Kjellbom P, Lamb CJ. Elicitor- and wound-induced oxidative cross-linking of a proline-rich plant cell wall protein: a novel, rapid defense response. Cell. 1992 Jul 10;70(1):21–30. [PubMed]
  • Brown JW. A catalogue of splice junction and putative branch point sequences from plant introns. Nucleic Acids Res. 1986 Dec 22;14(24):9549–9559. [PMC free article] [PubMed]
  • Buffard D, Breda C, van Huystee RB, Asemota O, Pierre M, Ha DB, Esnault R. Molecular cloning of complementary DNAs encoding two cationic peroxidases from cultivated peanut cells. Proc Natl Acad Sci U S A. 1990 Nov;87(22):8874–8878. [PMC free article] [PubMed]
  • Caetano-Anollés G, Gresshoff PM. Alfalfa Controls Nodulation during the Onset of Rhizobium-induced Cortical Cell Division. Plant Physiol. 1991 Feb;95(2):366–373. [PMC free article] [PubMed]
  • Criqui MC, Plesse B, Durr A, Marbach J, Parmentier Y, Jamet E, Fleck J. Characterization of genes expressed in mesophyll protoplasts of Nicotiana sylvestris before the re-initiation of the DNA replicational activity. Mech Dev. 1992 Aug;38(2):121–132. [PubMed]
  • Feinberg AP, Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. [PubMed]
  • Fisher RF, Long SR. Rhizobium--plant signal exchange. Nature. 1992 Jun 25;357(6380):655–660. [PubMed]
  • Gallusci P, Dedieu A, Journet EP, Huguet T, Barker DG. Synchronous expression of leghaemoglobin genes in Medicago truncatula during nitrogen-fixing root nodule development and response to exogenously supplied nitrate. Plant Mol Biol. 1991 Sep;17(3):335–349. [PubMed]
  • Horvath B, Heidstra R, Lados M, Moerman M, Spaink HP, Promé JC, van Kammen A, Bisseling T. Lipo-oligosaccharides of Rhizobium induce infection-related early nodulin gene expression in pea root hairs. Plant J. 1993 Oct;4(4):727–733. [PubMed]
  • Kondorosi E, Gyuris J, Schmidt J, John M, Duda E, Hoffmann B, Schell J, Kondorosi A. Positive and negative control of nod gene expression in Rhizobium meliloti is required for optimal nodulation. EMBO J. 1989 May;8(5):1331–1340. [PMC free article] [PubMed]
  • Kondorosi E, Pierre M, Cren M, Haumann U, Buiré M, Hoffmann B, Schell J, Kondorosi A. Identification of NolR, a negative transacting factor controlling the nod regulon in Rhizobium meliloti. J Mol Biol. 1991 Dec 20;222(4):885–896. [PubMed]
  • Kouchi H, Hata S. Isolation and characterization of novel nodulin cDNAs representing genes expressed at early stages of soybean nodule development. Mol Gen Genet. 1993 Apr;238(1-2):106–119. [PubMed]
  • Lerouge P, Roche P, Faucher C, Maillet F, Truchet G, Promé JC, Dénarié J. Symbiotic host-specificity of Rhizobium meliloti is determined by a sulphated and acylated glucosamine oligosaccharide signal. Nature. 1990 Apr 19;344(6268):781–784. [PubMed]
  • Löbler M, Hirsch AM. A gene that encodes a proline-rich nodulin with limited homology to PsENOD12 is expressed in the invasion zone of Rhizobium meliloti-induced alfalfa root nodules. Plant Physiol. 1993 Sep;103(1):21–30. [PMC free article] [PubMed]
  • Lütcke HA, Chow KC, Mickel FS, Moss KA, Kern HF, Scheele GA. Selection of AUG initiation codons differs in plants and animals. EMBO J. 1987 Jan;6(1):43–48. [PMC free article] [PubMed]
  • Mazza G, Welinder KG. Covalent structure of turnip peroxidase 7. Cyanogen bromide fragments, complete structure and comparison to horseradish peroxidase C. Eur J Biochem. 1980 Jul;108(2):481–489. [PubMed]
  • Pichon M, Journet EP, Dedieu A, de Billy F, Truchet G, Barker DG. Rhizobium meliloti elicits transient expression of the early nodulin gene ENOD12 in the differentiating root epidermis of transgenic alfalfa. Plant Cell. 1992 Oct;4(10):1199–1211. [PMC free article] [PubMed]
  • Reimmann C, Ringli C, Dudler R. Complementary DNA cloning and sequence analysis of a pathogen-induced putative peroxidase from rice. Plant Physiol. 1992 Nov;100(3):1611–1612. [PMC free article] [PubMed]
  • Roche P, Debellé F, Maillet F, Lerouge P, Faucher C, Truchet G, Dénarié J, Promé JC. Molecular basis of symbiotic host specificity in Rhizobium meliloti: nodH and nodPQ genes encode the sulfation of lipo-oligosaccharide signals. Cell. 1991 Dec 20;67(6):1131–1143. [PubMed]
  • Salzwedel JL, Dazzo FB. pSym nod gene influence on elicitation of peroxidase activity from white clover and pea roots by rhizobia and their cell-free supernatants. Mol Plant Microbe Interact. 1993 Jan-Feb;6(1):127–134. [PubMed]
  • Scheres B, Van De Wiel C, Zalensky A, Horvath B, Spaink H, Van Eck H, Zwartkruis F, Wolters AM, Gloudemans T, Van Kammen A, et al. The ENOD12 gene product is involved in the infection process during the pea-Rhizobium interaction. Cell. 1990 Jan 26;60(2):281–294. [PubMed]
  • van Brussel AA, Bakhuizen R, van Spronsen PC, Spaink HP, Tak T, Lugtenberg BJ, Kijne JW. Induction of pre-infection thread structures in the leguminous host plant by mitogenic lipo-oligosaccharides of Rhizobium. Science. 1992 Jul 3;257(5066):70–72. [PubMed]
  • Van Engelen FA, De Vries SC. Extracellular proteins in plant embryogenesis. Trends Genet. 1992 Feb;8(2):66–70. [PubMed]
  • von Heijne G. Patterns of amino acids near signal-sequence cleavage sites. Eur J Biochem. 1983 Jun 1;133(1):17–21. [PubMed]
  • Wang Z, Brown DD. A gene expression screen. Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11505–11509. [PMC free article] [PubMed]
  • Wilson RC, Long F, Maruoka EM, Cooper JB. A new proline-rich early nodulin from Medicago truncatula is highly expressed in nodule meristematic cells. Plant Cell. 1994 Sep;6(9):1265–1275. [PMC free article] [PubMed]

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