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Plant Physiol. Mar 1994; 104(3): 881–887.
PMCID: PMC160685

Isolation of an Arabidopsis thaliana Mutant, mto1, That Overaccumulates Soluble Methionine (Temporal and Spatial Patterns of Soluble Methionine Accumulation).

Abstract

We isolated Arabidopsis thaliana mutants that are resistant to ethionine, a toxic analog of methionine (Met). One of the mutants was analyzed further, and it accumulated 10- to 40-fold more soluble Met than the wild type in the aerial parts during the vegetative growth period. When the mutant plants started to flower, however, the soluble Met content in the rosette region decreased to the wild-type level, whereas that in the inflorescence apex region and in immature fruits was 5- to 8-fold higher than the wild type. These results indicate that the concentration of soluble Met is temporally and spatially regulated and suggest that soluble Met is translocated to sink organs after the onset of reproductive growth. The causal mutation, designated mto1, was a single, nuclear, semidominant mutation and mapped to chromosome 3. Accumulation profiles of soluble amino acids suggested that the mutation affects a later step(s) in the Met biosynthesis pathway. Ethylene production of the mutants was only 40% higher than the wild-type plants, indicating that ethylene production is tightly regulated at a step after Met synthesis. This mutant will be useful in studying the translocation of amino acids, as well as regulation of Met biosynthesis and other metabolic pathways related to Met.

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Selected References

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  • Alix JH. Molecular aspects of the in vivo and in vitro effects of ethionine, an analog of methionine. Microbiol Rev. 1982 Sep;46(3):281–295. [PMC free article] [PubMed]
  • Cherest H, Surdin-Kerjan Y, Antoniewski J, de Robichon-Szulmajster H. Effects of regulatory mutations upon methionine biosynthesis in Saccharomyces cerevisiae: loci eth2-eth3-eth10. J Bacteriol. 1973 Sep;115(3):1084–1093. [PMC free article] [PubMed]
  • Datko AH, Mudd SH, Giovanelli J, Macnicol PK. Sulfur-containing Compounds in Lemna perpusilla 6746 Grown at a Range of Sulfate Concentrations. Plant Physiol. 1978 Oct;62(4):629–635. [PMC free article] [PubMed]
  • Duke SH, Schrader LE, Miller MG. Low Temperature Effects on Soybean (Glycine max [L.] Merr. cv. Wells) Free Amino Acid Pools during Germination. Plant Physiol. 1978 Oct;62(4):642–647. [PMC free article] [PubMed]
  • Fujiwara T, Hirai MY, Chino M, Komeda Y, Naito S. Effects of sulfur nutrition on expression of the soybean seed storage protein genes in transgenic petunia. Plant Physiol. 1992 May;99(1):263–268. [PMC free article] [PubMed]
  • Giovanelli J, Mudd SH, Datko AH. In vivo regulation of de novo methionine biosynthesis in a higher plant (lemna). Plant Physiol. 1985 Feb;77(2):450–455. [PMC free article] [PubMed]
  • Gonzales RA, Das PK, Widholm JM. Characterization of cultured tobacco cell lines resistant to ethionine, a methionine analog. Plant Physiol. 1984 Mar;74(3):640–644. [PMC free article] [PubMed]
  • Guzmán P, Ecker JR. Exploiting the triple response of Arabidopsis to identify ethylene-related mutants. Plant Cell. 1990 Jun;2(6):513–523. [PMC free article] [PubMed]
  • Lawrence DA, Smith DA, Rowbury RJ. Regulation of methionine synthesis in Salmonella typhimurium: mutants resistant to inhibition by analogues of methionine. Genetics. 1968 Apr;58(4):473–492. [PMC free article] [PubMed]
  • Markham GD, DeParasis J, Gatmaitan J. The sequence of metK, the structural gene for S-adenosylmethionine synthetase in Escherichia coli. J Biol Chem. 1984 Dec 10;259(23):14505–14507. [PubMed]
  • Ooms JJJ, Leon-Kloosterziel KM, Bartels D, Koornneef M, Karssen CM. Acquisition of Desiccation Tolerance and Longevity in Seeds of Arabidopsis thaliana (A Comparative Study Using Abscisic Acid-Insensitive abi3 Mutants). Plant Physiol. 1993 Aug;102(4):1185–1191. [PMC free article] [PubMed]
  • Peleman J, Boerjan W, Engler G, Seurinck J, Botterman J, Alliotte T, Van Montagu M, Inzé D. Strong cellular preference in the expression of a housekeeping gene of Arabidopsis thaliana encoding S-adenosylmethionine synthetase. Plant Cell. 1989 Jan;1(1):81–93. [PMC free article] [PubMed]
  • Riens B, Lohaus G, Heineke D, Heldt HW. Amino Acid and sucrose content determined in the cytosolic, chloroplastic, and vacuolar compartments and in the Phloem sap of spinach leaves. Plant Physiol. 1991 Sep;97(1):227–233. [PMC free article] [PubMed]
  • Sloger M, Owens LD. Control of Free Methionine Production in Wild Type and Ethionine-resistant Mutants of Chlorella sorokiniana. Plant Physiol. 1974 Mar;53(3):469–473. [PMC free article] [PubMed]
  • Thomas D, Rothstein R, Rosenberg N, Surdin-Kerjan Y. SAM2 encodes the second methionine S-adenosyl transferase in Saccharomyces cerevisiae: physiology and regulation of both enzymes. Mol Cell Biol. 1988 Dec;8(12):5132–5139. [PMC free article] [PubMed]
  • Thompson GA, Datko AH, Mudd SH, Giovanelli J. Methionine Biosynthesis in Lemna: STUDIES ON THE REGULATION OF CYSTATHIONINE gamma-SYNTHASE, O-PHOSPHOHOMOSERINE SULFHYDRYLASE, AND O-ACETYLSERINE SULFHYDRYLASE. Plant Physiol. 1982 May;69(5):1077–1083. [PMC free article] [PubMed]
  • Winter H, Lohaus G, Heldt HW. Phloem Transport of Amino Acids in Relation to their Cytosolic Levels in Barley Leaves. Plant Physiol. 1992 Jul;99(3):996–1004. [PMC free article] [PubMed]

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