• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of plntcellLink to Publisher's site
Plant Cell. Dec 1994; 6(12): 1911–1922.
PMCID: PMC160571

Two Structural Domains Mediate Two Sequential Events in [gamma]-Zein Targeting: Protein Endoplasmic Reticulum Retention and Protein Body Formation.

Abstract

[gamma]-Zein is a maize storage protein synthesized by endosperm cells and stored together with [alpha]- and [beta]-zeins in specialized organelles called protein bodies. Previous studies have shown that in maize there is only one type of protein body and it is derived directly from the endoplasmic reticulum (ER). In this article, we describe the domains of [gamma]-zein involved in ER retention and the domains involved in protein body formation. To identify the signal responsible for [gamma]-zein retention in ER-derived protein bodies, DNAs encoding various deletion mutants of [gamma]-zein were constructed and introduced into Arabidopsis as a heterologous system. By using pulse-chase experiments and immunoelectron microscopy, we demonstrated that the deletion of a proline-rich domain at the N terminus of [gamma]-zein puts an end to its retention in the ER; this resulted in the secretion of the mutated protein. The amino acid sequence of [gamma]-zein necessary for ER retention is the repeat domain composed of eight units of the hexapeptide PPPVHL. In addition, we observed that only those [gamma]-zein mutants that contained both the proline-rich repeat domain and the C-terminal cysteine-rich domain were able to form ER-derived protein bodies. We suggest that the retention of [gamma]-zein in the ER could be a result of a protein-protein association or a transient interaction of the repeat domain with ER membranes.

Full Text

The Full Text of this article is available as a PDF (2.9M).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Bauerfeind R, Huttner WB. Biogenesis of constitutive secretory vesicles, secretory granules and synaptic vesicles. Curr Opin Cell Biol. 1993 Aug;5(4):628–635. [PubMed]
  • Bednarek SY, Raikhel NV. Intracellular trafficking of secretory proteins. Plant Mol Biol. 1992 Oct;20(1):133–150. [PubMed]
  • Bennett MK, Scheller RH. The molecular machinery for secretion is conserved from yeast to neurons. Proc Natl Acad Sci U S A. 1993 Apr 1;90(7):2559–2563. [PMC free article] [PubMed]
  • Bevan M. Binary Agrobacterium vectors for plant transformation. Nucleic Acids Res. 1984 Nov 26;12(22):8711–8721. [PMC free article] [PubMed]
  • Celma C, Albericio F, Pedroso E, Giralt E. Convergent solid-phase peptide synthesis IX: application to the synthesis of peptides with repetitive sequences. Pept Res. 1992 Jan-Feb;5(1):62–71. [PubMed]
  • Freedman RB. Protein disulfide isomerase: multiple roles in the modification of nascent secretory proteins. Cell. 1989 Jun 30;57(7):1069–1072. [PubMed]
  • Galili G, Altschuler Y, Levanony H. Assembly and transport of seed storage proteins. Trends Cell Biol. 1993 Dec;3(12):437–442. [PubMed]
  • Höfte H, Hubbard L, Reizer J, Ludevid D, Herman EM, Chrispeels MJ. Vegetative and Seed-Specific Forms of Tonoplast Intrinsic Protein in the Vacuolar Membrane of Arabidopsis thaliana. Plant Physiol. 1992 Jun;99(2):561–570. [PMC free article] [PubMed]
  • Jaenicke R. Protein folding: local structures, domains, subunits, and assemblies. Biochemistry. 1991 Apr 2;30(13):3147–3161. [PubMed]
  • Lending CR, Larkins BA. Changes in the zein composition of protein bodies during maize endosperm development. Plant Cell. 1989 Oct;1(10):1011–1023. [PMC free article] [PubMed]
  • Lewis MJ, Pelham HR. Ligand-induced redistribution of a human KDEL receptor from the Golgi complex to the endoplasmic reticulum. Cell. 1992 Jan 24;68(2):353–364. [PubMed]
  • Li X, Wu Y, Zhang DZ, Gillikin JW, Boston RS, Franceschi VR, Okita TW. Rice prolamine protein body biogenesis: a BiP-mediated process. Science. 1993 Nov 12;262(5136):1054–1056. [PubMed]
  • Moore PJ, Swords KM, Lynch MA, Staehelin LA. Spatial organization of the assembly pathways of glycoproteins and complex polysaccharides in the Golgi apparatus of plants. J Cell Biol. 1991 Feb;112(4):589–602. [PMC free article] [PubMed]
  • Munro S, Pelham HR. A C-terminal signal prevents secretion of luminal ER proteins. Cell. 1987 Mar 13;48(5):899–907. [PubMed]
  • Pietrzak M, Shillito RD, Hohn T, Potrykus I. Expression in plants of two bacterial antibiotic resistance genes after protoplast transformation with a new plant expression vector. Nucleic Acids Res. 1986 Jul 25;14(14):5857–5868. [PMC free article] [PubMed]
  • Prat S, Cortadas J, Puigdomènech P, Palau J. Nucleic acid (cDNA) and amino acid sequences of the maize endosperm protein glutelin-2. Nucleic Acids Res. 1985 Mar 11;13(5):1493–1504. [PMC free article] [PubMed]
  • Rabanal F, Ludevid MD, Pons M, Giralt E. CD of proline-rich polypeptides: application to the study of the repetitive domain of maize glutelin-2. Biopolymers. 1993 Jul;33(7):1019–1028. [PubMed]
  • Rubin R, Levanony H, Galili G. Evidence for the presence of two different types of protein bodies in wheat endosperm. Plant Physiol. 1992 Jun;99(2):718–724. [PMC free article] [PubMed]
  • Shani N, Steffen-Campbell JD, Anderson OD, Greene FC, Galili G. Role of the amino- and carboxy-terminal regions in the folding and oligomerization of wheat high molecular weight glutenin subunits. Plant Physiol. 1992 Feb;98(2):433–441. [PMC free article] [PubMed]
  • Shewry PR, Tatham AS. The prolamin storage proteins of cereal seeds: structure and evolution. Biochem J. 1990 Apr 1;267(1):1–12. [PMC free article] [PubMed]
  • Simon R, Altschuler Y, Rubin R, Galili G. Two Closely Related Wheat Storage Proteins Follow a Markedly Different Subcellular Route in Xenopus laevis Oocytes. Plant Cell. 1990 Sep;2(9):941–950. [PMC free article] [PubMed]
  • Sticher L, Hofsteenge J, Milani A, Neuhaus JM, Meins F., Jr Vacuolar chitinases of tobacco: a new class of hydroxyproline-containing proteins. Science. 1992 Jul 31;257(5070):655–657. [PubMed]
  • Torrent M, Geli MI, Ruiz-Avila L, Canals JM, Puigdomènech P, Ludevid D. Role of structural domains for maize gamma-zein retention in Xenopus oocytes. Planta. 1994;192(4):512–518. [PubMed]
  • Valvekens D, Montagu MV, Van Lijsebettens M. Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana root explants by using kanamycin selection. Proc Natl Acad Sci U S A. 1988 Aug;85(15):5536–5540. [PMC free article] [PubMed]
  • Voorberg J, Fontijn R, Calafat J, Janssen H, van Mourik JA, Pannekoek H. Biogenesis of von Willebrand factor-containing organelles in heterologous transfected CV-1 cells. EMBO J. 1993 Feb;12(2):749–758. [PMC free article] [PubMed]
  • Wieland FT, Gleason ML, Serafini TA, Rothman JE. The rate of bulk flow from the endoplasmic reticulum to the cell surface. Cell. 1987 Jul 17;50(2):289–300. [PubMed]
  • Williamson MP. The structure and function of proline-rich regions in proteins. Biochem J. 1994 Jan 15;297(Pt 2):249–260. [PMC free article] [PubMed]

Articles from The Plant Cell are provided here courtesy of American Society of Plant Biologists

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...