• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of plntcellLink to Publisher's site
Plant Cell. Nov 1993; 5(11): 1661–1668.
PMCID: PMC160394

Relationship between Endopolyploidy and Cell Size in Epidermal Tissue of Arabidopsis.


Relative quantities of DNA in individual nuclei of stem and leaf epidermal cells of Arabidopsis were measured microspectrofluorometrically using epidermal peels. The relative ploidy level in each nucleus was assessed by comparison to root tip mitotic nuclei. A clear pattern of regular endopolyploidy is evident in epidermal cells. Guard cell nuclei contain levels of DNA comparable to dividing root cells, the 2C level (i.e., one unreplicated copy of the nuclear DNA). Leaf trichome nuclei had elevated ploidy levels of 4C, 8C, 16C, 32C, and 64C, and their cytology suggested that the polyploidy represents a form of polyteny. The nuclei of epidermal pavement cells were 2C, 4C, and 8C in stem epidermis, and 2C, 4C, 8C, and 16C in leaf epidermis. Morphometry of epidermal pavement cells revealed a direct proportionality between nuclear DNA level and cell size. A consideration of the development process suggests that the cells of highest ploidy level are developmentally oldest; consequently, the developmental pattern of epidermal tissues can be read from the ploidy pattern of the cells. This observation is relevant to theories of stomate spacing and offers opportunities for genetic analysis of the endopolyploidy/polyteny phenomenon.

Full Text

The Full Text of this article is available as a PDF (1.5M).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Coleman AW, Maguire MJ, Coleman JR. Mithramycin- and 4'-6-diamidino-2-phenylindole (DAPI)-DNA staining for fluorescence microspectrophotometric measurement of DNA in nuclei, plastids, and virus particles. J Histochem Cytochem. 1981 Aug;29(8):959–968. [PubMed]
  • Croxdale J, Smith J, Yandell B, Johnson JB. Stomatal patterning in Tradescantia: an evaluation of the cell lineage theory. Dev Biol. 1992 Jan;149(1):158–167. [PubMed]
  • De Rocher EJ, Harkins KR, Galbraith DW, Bohnert HJ. Developmentally regulated systemic endopolyploid in succulents with small genomes. Science. 1990 Oct 5;250(4977):99–101. [PubMed]
  • Galbraith DW, Harkins KR, Knapp S. Systemic Endopolyploidy in Arabidopsis thaliana. Plant Physiol. 1991 Jul;96(3):985–989. [PMC free article] [PubMed]
  • Kausch AP, Horner HT. Increased nuclear DNA content in raphide crystal idioblasts during development in Vanilla planifolia L. (Orchidaceae). Eur J Cell Biol. 1984 Jan;33(1):7–12. [PubMed]

Articles from The Plant Cell are provided here courtesy of American Society of Plant Biologists


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...