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Plant Cell. Nov 1993; 5(11): 1575–1590.
PMCID: PMC160387

Identification and characterization of MPG1, a gene involved in pathogenicity from the rice blast fungus Magnaporthe grisea.

Abstract

Differential cDNA cloning was used to identify genes expressed during infectious growth of the fungal pathogen Magnaporthe grisea in its host, the rice plant. We characterized one of these genes, MPG1, in detail. Using a novel assay to determine the proportion of fungal biomass present in the plant, we determined that the MPG1 transcript was 60-fold more abundant during growth in the plant than in culture. Mpg1 mutants have a reduced ability to cause disease symptoms that appears to result from an impaired ability to undergo appressorium formation. MPG1 mRNA was highly abundant very early in plant infection concomitant with appressorium formation and was also abundant at the time of symptom development. The MPG1 mRNA was also expressed during conidiation and in mycelial cultures starved for nitrogen or carbon. MPG1 potentially encodes a small, secreted, cysteine-rich, moderately hydrophobic protein with the characteristics of a fungal hydrophobin. Consistent with the role of the MPG1 gene product as a hydrophobin, Mpg1 mutants show an "easily wettable" phenotype. Our results suggest that hydrophobins may have a role in the elaboration of infective structures by fungi and may fulfill other functions in fungal phytopathogenesis.

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Selected References

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  • Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol. 1990 Oct 5;215(3):403–410. [PubMed]
  • Ballance DJ. Sequences important for gene expression in filamentous fungi. Yeast. 1986 Dec;2(4):229–236. [PubMed]
  • Bell-Pedersen D, Dunlap JC, Loros JJ. The Neurospora circadian clock-controlled gene, ccg-2, is allelic to eas and encodes a fungal hydrophobin required for formation of the conidial rodlet layer. Genes Dev. 1992 Dec;6(12A):2382–2394. [PubMed]
  • Bolyard MG, Sticklen MB. Expression of a modified Dutch elm disease toxin in Escherichia coli. Mol Plant Microbe Interact. 1992 Nov-Dec;5(6):520–524. [PubMed]
  • Chasan R. Building Hyphae in the Air. Plant Cell. 1991 Oct;3(10):1048–1050. [PMC free article] [PubMed]
  • Crawford MS, Chumley FG, Weaver CG, Valent B. Characterization of the Heterokaryotic and Vegetative Diploid Phases of MAGNAPORTHE GRISEA. Genetics. 1986 Dec;114(4):1111–1129. [PMC free article] [PubMed]
  • Devereux J, Haeberli P, Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. [PMC free article] [PubMed]
  • Feinberg AP, Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. [PubMed]
  • Hamer JE, Givan S. Genetic mapping with dispersed repeated sequences in the rice blast fungus: mapping the SMO locus. Mol Gen Genet. 1990 Sep;223(3):487–495. [PubMed]
  • Hamer JE, Howard RJ, Chumley FG, Valent B. A mechanism for surface attachment in spores of a plant pathogenic fungus. Science. 1988 Jan 15;239(4837):288–290. [PubMed]
  • Hamer JE, Farrall L, Orbach MJ, Valent B, Chumley FG. Host species-specific conservation of a family of repeated DNA sequences in the genome of a fungal plant pathogen. Proc Natl Acad Sci U S A. 1989 Dec;86(24):9981–9985. [PMC free article] [PubMed]
  • von Heijne G. Patterns of amino acids near signal-sequence cleavage sites. Eur J Biochem. 1983 Jun 1;133(1):17–21. [PubMed]
  • Henikoff S. Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene. 1984 Jun;28(3):351–359. [PubMed]
  • Howard RJ, Ferrari MA, Roach DH, Money NP. Penetration of hard substrates by a fungus employing enormous turgor pressures. Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11281–11284. [PMC free article] [PubMed]
  • Kyte J, Doolittle RF. A simple method for displaying the hydropathic character of a protein. J Mol Biol. 1982 May 5;157(1):105–132. [PubMed]
  • Lauter FR, Russo VE, Yanofsky C. Developmental and light regulation of eas, the structural gene for the rodlet protein of Neurospora. Genes Dev. 1992 Dec;6(12A):2373–2381. [PubMed]
  • Leung H, Lehtinen U, Karjalainen R, Skinner D, Tooley P, Leong S, Ellingboe A. Transformation of the rice blast fungus Magnaporthe grisea to hygromycin B resistance. Curr Genet. 1990 May;17(5):409–411. [PubMed]
  • Marck C. 'DNA Strider': a 'C' program for the fast analysis of DNA and protein sequences on the Apple Macintosh family of computers. Nucleic Acids Res. 1988 Mar 11;16(5):1829–1836. [PMC free article] [PubMed]
  • Parsons KA, Chumley FG, Valent B. Genetic transformation of the fungal pathogen responsible for rice blast disease. Proc Natl Acad Sci U S A. 1987 Jun;84(12):4161–4165. [PMC free article] [PubMed]
  • Punt PJ, Oliver RP, Dingemanse MA, Pouwels PH, van den Hondel CA. Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli. Gene. 1987;56(1):117–124. [PubMed]
  • St Leger RJ, Staples RC, Roberts DW. Cloning and regulatory analysis of starvation-stress gene, ssgA, encoding a hydrophobin-like protein from the entomopathogenic fungus, Metarhizium anisopliae. Gene. 1992 Oct 12;120(1):119–124. [PubMed]
  • Stringer MA, Dean RA, Sewall TC, Timberlake WE. Rodletless, a new Aspergillus developmental mutant induced by directed gene inactivation. Genes Dev. 1991 Jul;5(7):1161–1171. [PubMed]
  • Sweigard JA, Chumley FG, Valent B. Cloning and analysis of CUT1, a cutinase gene from Magnaporthe grisea. Mol Gen Genet. 1992 Mar;232(2):174–182. [PubMed]
  • Talbot NJ, Salch YP, Ma M, Hamer JE. Karyotypic Variation within Clonal Lineages of the Rice Blast Fungus, Magnaporthe grisea. Appl Environ Microbiol. 1993 Feb;59(2):585–593. [PMC free article] [PubMed]
  • Timberlake WE. Developmental gene regulation in Aspergillus nidulans. Dev Biol. 1980 Aug;78(2):497–510. [PubMed]
  • Valent B, Farrall L, Chumley FG. Magnaporthe grisea genes for pathogenicity and virulence identified through a series of backcrosses. Genetics. 1991 Jan;127(1):87–101. [PMC free article] [PubMed]
  • Wessels JG, de Vries OM, Asgeirsdóttir SA, Springer J. The thn mutation of Schizophyllum commune, which suppresses formation of aerial hyphae, affects expression of the Sc3 hydrophobin gene. J Gen Microbiol. 1991 Oct;137(10):2439–2445. [PubMed]
  • Wosten HAB, De Vries OMH, Wessels JGH. Interfacial Self-Assembly of a Fungal Hydrophobin into a Hydrophobic Rodlet Layer. Plant Cell. 1993 Nov;5(11):1567–1574. [PMC free article] [PubMed]

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