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Plant Cell. 1993 Jun; 5(6): 657–666.
PMCID: PMC160303

An alternative transcript of the S locus glycoprotein gene in a class II pollen-recessive self-incompatibility haplotype of Brassica oleracea encodes a membrane-anchored protein.


Recent reports have shown that SLG, one of two genes linked to the S locus of Brassica, encodes a secreted glycoprotein. We have used RNA gel blot analysis, genomic and cDNA clone analysis, expression in transgenic plants, and immunodetection to characterize SLG2, the SLG gene derived from the S2 haplotype. This haplotype belongs to the class II group of S haplotypes that exhibit a weak incompatibility phenotype and are pollen recessive. We showed that SLG2 produces two transcript forms: the expected 1.6-kb transcript that predicts a secreted glycoprotein and an alternative 1.8-kb transcript that predicts a membrane-anchored protein. Stigmas of the S2 haplotype and pistils of transgenic tobacco plants transformed with the SLG2 gene produce a membrane-associated 62-kD protein as well as soluble 57- and 58-kD glycoforms. Because of the sequence similarity between SLG2 and the extracellular domain of the S Locus Receptor Kinase (SRK2) gene, the membrane-anchored form of SLG2 may be viewed as a naturally occurring truncated form of the receptor that lacks the kinase catalytic domain. The occurrence of this protein has potential implications for the activity of the full-length receptor. Furthermore, the underlying structure of the SLG2 gene suggests the evolution of SLG from an ancestral SRK-like gene.

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Selected References

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  • Amaya E, Musci TJ, Kirschner MW. Expression of a dominant negative mutant of the FGF receptor disrupts mesoderm formation in Xenopus embryos. Cell. 1991 Jul 26;66(2):257–270. [PubMed]
  • Bevan M. Binary Agrobacterium vectors for plant transformation. Nucleic Acids Res. 1984 Nov 26;12(22):8711–8721. [PMC free article] [PubMed]
  • Boyes DC, Nasrallah JB. Physical linkage of the SLG and SRK genes at the self-incompatibility locus of Brassica oleracea. Mol Gen Genet. 1993 Jan;236(2-3):369–373. [PubMed]
  • Chen CH, Nasrallah JB. A new class of S sequences defined by a pollen recessive self-incompatibility allele of Brassica oleracea. Mol Gen Genet. 1990 Jul;222(2-3):241–248. [PubMed]
  • Del Sal G, Manfioletti G, Schneider C. The CTAB-DNA precipitation method: a common mini-scale preparation of template DNA from phagemids, phages or plasmids suitable for sequencing. Biotechniques. 1989 May;7(5):514–520. [PubMed]
  • Dwyer KG, Balent MA, Nasrallah JB, Nasrallah ME. DNA sequences of self-incompatibility genes from Brassica campestris and B. oleracea: polymorphism predating speciation. Plant Mol Biol. 1991 Mar;16(3):481–486. [PubMed]
  • Gaude T, Friry A, Heizmann P, Mariac C, Rougier M, Fobis I, Dumas C. Expression of a self-incompatibility gene in a self-compatible line of Brassica oleracea. Plant Cell. 1993 Jan;5(1):75–86. [PMC free article] [PubMed]
  • Goring DR, Rothstein SJ. The S-locus receptor kinase gene in a self-incompatible Brassica napus line encodes a functional serine/threonine kinase. Plant Cell. 1992 Oct;4(10):1273–1281. [PMC free article] [PubMed]
  • Goring DR, Banks P, Fallis L, Baszczynski CL, Beversdorf WD, Rothstein SJ. Identification of an S-locus glycoprotein allele introgressed from B. napus ssp. rapifera to B. napus ssp. oleifera. Plant J. 1992 Nov;2(6):983–989. [PubMed]
  • Hemmati-Brivanlou A, Melton DA. A truncated activin receptor inhibits mesoderm induction and formation of axial structures in Xenopus embryos. Nature. 1992 Oct 15;359(6396):609–614. [PubMed]
  • Hood EE, Helmer GL, Fraley RT, Chilton MD. The hypervirulence of Agrobacterium tumefaciens A281 is encoded in a region of pTiBo542 outside of T-DNA. J Bacteriol. 1986 Dec;168(3):1291–1301. [PMC free article] [PubMed]
  • Kandasamy MK, Paolillo DJ, Faraday CD, Nasrallah JB, Nasrallah ME. The S-locus specific glycoproteins of Brassica accumulate in the cell wall of developing stigma papillae. Dev Biol. 1989 Aug;134(2):462–472. [PubMed]
  • Massagué J. Receptors for the TGF-beta family. Cell. 1992 Jun 26;69(7):1067–1070. [PubMed]
  • Moore HM, Nasrallah JB. A Brassica Self-Incompatibility Gene Is Expressed in the Stylar Transmitting Tissue of Transgenic Tobacco. Plant Cell. 1990 Jan;2(1):29–38. [PMC free article] [PubMed]
  • Nasrallah JB, Yu SM, Nasrallah ME. Self-incompatibility genes of Brassica oleracea: Expression, isolation, and structure. Proc Natl Acad Sci U S A. 1988 Aug;85(15):5551–5555. [PMC free article] [PubMed]
  • Redemann N, Holzmann B, von Rüden T, Wagner EF, Schlessinger J, Ullrich A. Anti-oncogenic activity of signalling-defective epidermal growth factor receptor mutants. Mol Cell Biol. 1992 Feb;12(2):491–498. [PMC free article] [PubMed]
  • Sato T, Thorsness MK, Kandasamy MK, Nishio T, Hirai M, Nasrallah JB, Nasrallah ME. Activity of an S Locus Gene Promoter in Pistils and Anthers of Transgenic Brassica. Plant Cell. 1991 Sep;3(9):867–876. [PMC free article] [PubMed]
  • Stein JC, Nasrallah JB. A plant receptor-like gene, the S-locus receptor kinase of Brassica oleracea L., encodes a functional serine/threonine kinase. Plant Physiol. 1993 Mar;101(3):1103–1106. [PMC free article] [PubMed]
  • Stein JC, Howlett B, Boyes DC, Nasrallah ME, Nasrallah JB. Molecular cloning of a putative receptor protein kinase gene encoded at the self-incompatibility locus of Brassica oleracea. Proc Natl Acad Sci U S A. 1991 Oct 1;88(19):8816–8820. [PMC free article] [PubMed]
  • Ullrich A, Schlessinger J. Signal transduction by receptors with tyrosine kinase activity. Cell. 1990 Apr 20;61(2):203–212. [PubMed]
  • Umbach AL, Lalonde BA, Kandasamy MK, Nasrallah JB, Nasrallah ME. Immunodetection of protein glycoforms encoded by two independent genes of the self-incompatibility multigene family of brassica. Plant Physiol. 1990 Jun;93(2):739–747. [PMC free article] [PubMed]
  • von Heijne G, Gavel Y. Topogenic signals in integral membrane proteins. Eur J Biochem. 1988 Jul 1;174(4):671–678. [PubMed]

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