• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of plntcellLink to Publisher's site
Plant Cell. Nov 1992; 4(11): 1425–1433.
PMCID: PMC160229

Two endogenous proteins that induce cell wall extension in plants.


Plant cell enlargement is regulated by wall relaxation and yielding, which is thought to be catalyzed by elusive "wall-loosening" enzymes. By employing a reconstitution approach, we found that a crude protein extract from the cell walls of growing cucumber seedlings possessed the ability to induce the extension of isolated cell walls. This activity was restricted to the growing region of the stem and could induce the extension of isolated cell walls from various dicot stems and the leaves of amaryllidaceous monocots, but was less effective on grass coleoptile walls. Endogenous and reconstituted wall extension activities showed similar sensitivities to pH, metal ions, thiol reducing agents, proteases, and boiling in methanol or water. Sequential HPLC fractionation of the active wall extract revealed two proteins with molecular masses of 29 and 30 kD associated with the activity. Each protein, by itself, could induce wall extension without detectable hydrolytic breakdown of the wall. These proteins appear to mediate "acid growth" responses of isolated walls and may catalyze plant cell wall extension by a novel biochemical mechanism.

Full Text

The Full Text of this article is available as a PDF (1.1M).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Cleland RE, Cosgrove D, Tepfer M. Long-term acid-induced wall extension in an in-vitro system. Planta. 1987;170:379–385. [PubMed]
  • Cosgrove DJ. Wall relaxation and the driving forces for cell expansive growth. Plant Physiol. 1987;84:561–564. [PMC free article] [PubMed]
  • Cosgrove DJ. Characterization of long-term extension of isolated cell walls from growing cucumber hypocotyls. Planta. 1989;177:121–130. [PubMed]
  • Inouhe M, Nevins DJ. Inhibition of auxin-induced cell elongation of maize coleoptiles by antibodies specific for cell wall glucanases. Plant Physiol. 1991 Jun;96(2):426–431. [PMC free article] [PubMed]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed]
  • Lüthen H, Bigdon M, Böttger M. Reexamination of the Acid growth theory of auxin action. Plant Physiol. 1990 Jul;93(3):931–939. [PMC free article] [PubMed]
  • McClure BA, Hagen G, Brown CS, Gee MA, Guilfoyle TJ. Transcription, organization, and sequence of an auxin-regulated gene cluster in soybean. Plant Cell. 1989 Feb;1(2):229–239. [PMC free article] [PubMed]
  • McNeil M, Darvill AG, Fry SC, Albersheim P. Structure and function of the primary cell walls of plants. Annu Rev Biochem. 1984;53:625–663. [PubMed]
  • Rayle DL, Cleland RE. The Acid Growth Theory of auxin-induced cell elongation is alive and well. Plant Physiol. 1992 Aug;99(4):1271–1274. [PMC free article] [PubMed]
  • Rayle DL, Haughton PM, Cleland R. An in vitro system that simulates plant cell extension growth. Proc Natl Acad Sci U S A. 1970 Dec;67(4):1814–1817. [PMC free article] [PubMed]
  • Schopfer P. pH-Dependence of Extension Growth in Avena Coleoptiles and Its Implications for the Mechanism of Auxin Action. Plant Physiol. 1989 May;90(1):202–207. [PMC free article] [PubMed]

Articles from The Plant Cell are provided here courtesy of American Society of Plant Biologists


Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


  • PubMed
    PubMed citations for these articles

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...