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Plant Cell. Nov 1992; 4(11): 1359–1369.
PMCID: PMC160224

Functional homologs of the Arabidopsis RPM1 disease resistance gene in bean and pea.

Abstract

We showed that a bacterial avirulence (avr) gene function, avrPpiA1, from the pea pathogen Pseudomonas syringae pv pisi, is recognized by some, but not all, genotypes of Arabidopsis. Thus, an avr gene functionally defined on a crop species is also an avr gene on Arabidopsis. The activity of avrPpiA1 on a series of Arabidopsis genotypes is identical to that of the avrRpm1 gene from P.s. pv maculicola previously defined using Arabidopsis. The two avr genes are homologous and encode nearly identical predicted products. Moreover, this conserved avr function is also recognized by some bean and pea cultivars in what has been shown to be a gene-for-gene manner. We further demonstrated that the Arabidopsis disease resistance locus, RPM1, conditioning resistance to avrRpm1, also conditions resistance to bacterial strains carrying avrPpiA1. Therefore, bean, pea, and conceivably other crop species contain functional and potentially molecular homologs of RPM1.

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Selected References

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  • Bavage AD, Vivian A, Atherton GT, Taylor JD, Malik AN. Molecular genetics of Pseudomonas syringae pathovar pisi: plasmid involvement in cultivar-specific incompatibility. J Gen Microbiol. 1991 Sep;137(9):2231–2239. [PubMed]
  • Boyer HW, Roulland-Dussoix D. A complementation analysis of the restriction and modification of DNA in Escherichia coli. J Mol Biol. 1969 May 14;41(3):459–472. [PubMed]
  • Dong X, Mindrinos M, Davis KR, Ausubel FM. Induction of Arabidopsis defense genes by virulent and avirulent Pseudomonas syringae strains and by a cloned avirulence gene. Plant Cell. 1991 Jan;3(1):61–72. [PMC free article] [PubMed]
  • Figurski DH, Helinski DR. Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1648–1652. [PMC free article] [PubMed]
  • Frank SA. Models of plant-pathogen coevolution. Trends Genet. 1992 Jun;8(6):213–219. [PubMed]
  • Jenner C, Hitchin E, Mansfield J, Walters K, Betteridge P, Teverson D, Taylor J. Gene-for-gene interactions between Pseudomonas syringae pv. phaseolicola and Phaseolus. Mol Plant Microbe Interact. 1991 Nov-Dec;4(6):553–562. [PubMed]
  • Kado CI, Liu ST. Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol. 1981 Mar;145(3):1365–1373. [PMC free article] [PubMed]
  • Kearney B, Staskawicz BJ. Widespread distribution and fitness contribution of Xanthomonas campestris avirulence gene avrBs2. Nature. 1990 Jul 26;346(6282):385–386. [PubMed]
  • Keen NT. Gene-for-gene complementarity in plant-pathogen interactions. Annu Rev Genet. 1990;24:447–463. [PubMed]
  • Keen NT, Tamaki S, Kobayashi D, Trollinger D. Improved broad-host-range plasmids for DNA cloning in gram-negative bacteria. Gene. 1988 Oct 15;70(1):191–197. [PubMed]
  • KING EO, WARD MK, RANEY DE. Two simple media for the demonstration of pyocyanin and fluorescin. J Lab Clin Med. 1954 Aug;44(2):301–307. [PubMed]
  • Kobayashi DY, Tamaki SJ, Keen NT. Molecular characterization of avirulence gene D from Pseudomonas syringae pv. tomato. Mol Plant Microbe Interact. 1990 Mar-Apr;3(2):94–102. [PubMed]
  • Koch E, Slusarenko A. Arabidopsis is susceptible to infection by a downy mildew fungus. Plant Cell. 1990 May;2(5):437–445. [PMC free article] [PubMed]
  • Lindgren PB, Frederick R, Govindarajan AG, Panopoulos NJ, Staskawicz BJ, Lindow SE. An ice nucleation reporter gene system: identification of inducible pathogenicity genes in Pseudomonas syringae pv. phaseolicola. EMBO J. 1989 May;8(5):1291–1301. [PMC free article] [PubMed]
  • Shepherd KW, Mayo GM. Genes conferring specific plant disease resistance. Science. 1972 Jan 28;175(4020):375–380. [PubMed]
  • Stachel SE, An G, Flores C, Nester EW. A Tn3 lacZ transposon for the random generation of beta-galactosidase gene fusions: application to the analysis of gene expression in Agrobacterium. EMBO J. 1985 Apr;4(4):891–898. [PMC free article] [PubMed]
  • Staskawicz BJ, Dahlbeck D, Keen NT. Cloned avirulence gene of Pseudomonas syringae pv. glycinea determines race-specific incompatibility on Glycine max (L.) Merr. Proc Natl Acad Sci U S A. 1984 Oct;81(19):6024–6028. [PMC free article] [PubMed]
  • Tamaki S, Dahlbeck D, Staskawicz B, Keen NT. Characterization and expression of two avirulence genes cloned from Pseudomonas syringae pv. glycinea. J Bacteriol. 1988 Oct;170(10):4846–4854. [PMC free article] [PubMed]
  • Uknes S, Mauch-Mani B, Moyer M, Potter S, Williams S, Dincher S, Chandler D, Slusarenko A, Ward E, Ryals J. Acquired resistance in Arabidopsis. Plant Cell. 1992 Jun;4(6):645–656. [PMC free article] [PubMed]
  • Valent B, Farrall L, Chumley FG. Magnaporthe grisea genes for pathogenicity and virulence identified through a series of backcrosses. Genetics. 1991 Jan;127(1):87–101. [PMC free article] [PubMed]
  • Whalen MC, Stall RE, Staskawicz BJ. Characterization of a gene from a tomato pathogen determining hypersensitive resistance in non-host species and genetic analysis of this resistance in bean. Proc Natl Acad Sci U S A. 1988 Sep;85(18):6743–6747. [PMC free article] [PubMed]
  • Whalen MC, Innes RW, Bent AF, Staskawicz BJ. Identification of Pseudomonas syringae pathogens of Arabidopsis and a bacterial locus determining avirulence on both Arabidopsis and soybean. Plant Cell. 1991 Jan;3(1):49–59. [PMC free article] [PubMed]

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