Logo of plntcellLink to Publisher's site
Plant Cell. 1990 Sep; 2(9): 867–876.
PMCID: PMC159937

Cloning of the y1 Locus of Maize, a Gene Involved in the Biosynthesis of Carotenoids.


The y1 gene is one of the genes responsible for the production of [beta]-carotene in the endosperm and leaves of maize. We have cloned a Robertson's Mutator-tagged allele of the y1 gene (y1-mum) by using a Mu3 element as a hybridization probe. We substantiate that the cloned sequence is a portion of the y1 gene by molecular analyses of a revertant of a putative Mutator-induced y1 allele and the incidence of insertions within the cloned y1 sequence from several independently derived Mutator-induced y1 mutant stocks. The y1-mum sequence was used to isolate the standard Y1 allele, which conditions the presence of [beta]-carotene in the endosperm of the maize kernel.

Full Text

The Full Text of this article is available as a PDF (3.7M).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Barker RF, Thompson DV, Talbot DR, Swanson J, Bennetzen JL. Nucleotide sequence of the maize transposable element Mul. Nucleic Acids Res. 1984 Aug 10;12(15):5955–5967. [PMC free article] [PubMed]
  • Buckner B, Novotny CP, Ullrich RC. Organization of the ribosomal RNA genes of Schizophyllum commune. Curr Genet. 1988 May;13(5):417–424. [PubMed]
  • Cone KC, Burr FA, Burr B. Molecular analysis of the maize anthocyanin regulatory locus C1. Proc Natl Acad Sci U S A. 1986 Dec;83(24):9631–9635. [PMC free article] [PubMed]
  • Fedoroff NV, Furtek DB, Nelson OE. Cloning of the bronze locus in maize by a simple and generalizable procedure using the transposable controlling element Activator (Ac). Proc Natl Acad Sci U S A. 1984 Jun;81(12):3825–3829. [PMC free article] [PubMed]
  • Hake S, Vollbrecht E, Freeling M. Cloning Knotted, the dominant morphological mutant in maize using Ds2 as a transposon tag. EMBO J. 1989 Jan;8(1):15–22. [PMC free article] [PubMed]
  • Hartings H, Maddaloni M, Lazzaroni N, Di Fonzo N, Motto M, Salamini F, Thompson R. The O2 gene which regulates zein deposition in maize endosperm encodes a protein with structural homologies to transcriptional activators. EMBO J. 1989 Oct;8(10):2795–2801. [PMC free article] [PubMed]
  • Ludwig SR, Habera LF, Dellaporta SL, Wessler SR. Lc, a member of the maize R gene family responsible for tissue-specific anthocyanin production, encodes a protein similar to transcriptional activators and contains the myc-homology region. Proc Natl Acad Sci U S A. 1989 Sep;86(18):7092–7096. [PMC free article] [PubMed]
  • Martienssen RA, Barkan A, Freeling M, Taylor WC. Molecular cloning of a maize gene involved in photosynthetic membrane organization that is regulated by Robertson's Mutator. EMBO J. 1989 Jun;8(6):1633–1639. [PMC free article] [PubMed]
  • McCarty DR, Carson CB, Stinard PS, Robertson DS. Molecular Analysis of viviparous-1: An Abscisic Acid-Insensitive Mutant of Maize. Plant Cell. 1989 May;1(5):523–532. [PMC free article] [PubMed]
  • McLaughlin M, Walbot V. Cloning of a mutable bz2 allele of maize by transposon tagging and differential hybridization. Genetics. 1987 Dec;117(4):771–776. [PMC free article] [PubMed]
  • O'Reilly C, Shepherd NS, Pereira A, Schwarz-Sommer Z, Bertram I, Robertson DS, Peterson PA, Saedler H. Molecular cloning of the a1 locus of Zea mays using the transposable elements En and Mu1. EMBO J. 1985 Apr;4(4):877–882. [PMC free article] [PubMed]
  • Schmidt RJ, Burr FA, Burr B. Transposon tagging and molecular analysis of the maize regulatory locus opaque-2. Science. 1987 Nov 13;238(4829):960–963. [PubMed]
  • Talbert LE, Patterson GI, Chandler VL. Mu transposable elements are structurally diverse and distributed throughout the genus Zea. J Mol Evol. 1989 Jul;29(1):28–39. [PubMed]
  • Theres N, Scheele T, Starlinger P. Cloning of the Bz2 locus of Zea mays using the transposable element Ds as a gene tag. Mol Gen Genet. 1987 Aug;209(1):193–197. [PubMed]

Articles from The Plant Cell are provided here courtesy of American Society of Plant Biologists


Save items

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...


  • MedGen
    Related information in MedGen
  • PubMed
    PubMed citations for these articles

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...