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Plant Cell. Feb 1989; 1(2): 173–179.
PMCID: PMC159749

Characterization of a pollen-specific cDNA clone from Zea mays and its expression.

Abstract

A pollen-specific cDNA clone, Zmc13, has been isolated from a cDNA library constructed to poly(A) RNA from mature maize pollen. The cDNA as shown by primer extension analysis is a full-length copy of the mRNA. The cDNA has been sequenced and is 929 nucleotides in length plus a 47-nucleotide poly(A) tail. Putative polyadenylation signals are identifiable in the 3'-nontranslated region. The mRNA codes for a predicted polypeptide containing 170 amino acid residues and with a molecular mass of 18.3 kilodaltons. The hydropathy profile suggests a possible signal sequence on the amino terminus. A comparison of the nucleotide and deduced amino acid sequence with sequences in data banks has not shown homology to known molecules. In situ hybridizations using RNA probes show that the mRNA is located in the cytoplasm of the vegetative cell of the pollen grain and after germination is distributed throughout the pollen tube cytoplasm.

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Selected References

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  • Belfort M, Pedersen-Lane J, West D, Ehrenman K, Maley G, Chu F, Maley F. Processing of the intron-containing thymidylate synthase (td) gene of phage T4 is at the RNA level. Cell. 1985 Jun;41(2):375–382. [PubMed]
  • Cox KH, DeLeon DV, Angerer LM, Angerer RC. Detection of mrnas in sea urchin embryos by in situ hybridization using asymmetric RNA probes. Dev Biol. 1984 Feb;101(2):485–502. [PubMed]
  • Geraghty D, Peifer MA, Rubenstein I, Messing J. The primary structure of a plant storage protein: zein. Nucleic Acids Res. 1981 Oct 10;9(19):5163–5174. [PMC free article] [PubMed]
  • Hyldig-Nielsen JJ, Jensen EO, Paludan K, Wiborg O, Garrett R, Jørgensen P, Marcker KA. The primary structures of two leghemoglobin genes from soybean. Nucleic Acids Res. 1982 Jan 22;10(2):689–701. [PMC free article] [PubMed]
  • Joshi CP. An inspection of the domain between putative TATA box and translation start site in 79 plant genes. Nucleic Acids Res. 1987 Aug 25;15(16):6643–6653. [PMC free article] [PubMed]
  • Joshi CP. Putative polyadenylation signals in nuclear genes of higher plants: a compilation and analysis. Nucleic Acids Res. 1987 Dec 10;15(23):9627–9640. [PMC free article] [PubMed]
  • Kyte J, Doolittle RF. A simple method for displaying the hydropathic character of a protein. J Mol Biol. 1982 May 5;157(1):105–132. [PubMed]
  • Lütcke HA, Chow KC, Mickel FS, Moss KA, Kern HF, Scheele GA. Selection of AUG initiation codons differs in plants and animals. EMBO J. 1987 Jan;6(1):43–48. [PMC free article] [PubMed]
  • Lycett GW, Croy RR, Shirsat AH, Boulter D. The complete nucleotide sequence of a legumin gene from pea (Pisum sativum L.). Nucleic Acids Res. 1984 Jun 11;12(11):4493–4506. [PMC free article] [PubMed]
  • Sachs MM, Dennis ES, Gerlach WL, Peacock WJ. Two Alleles of Maize ALCOHOL DEHYDROGENASE 1 Have 3' Structural and Poly(a) Addition Polymorphisms. Genetics. 1986 Jun;113(2):449–467. [PMC free article] [PubMed]
  • Stinson JR, Eisenberg AJ, Willing RP, Pe ME, Hanson DD, Mascarenhas JP. Genes expressed in the male gametophyte of flowering plants and their isolation. Plant Physiol. 1987 Feb;83(2):442–447. [PMC free article] [PubMed]
  • Willing RP, Mascarenhas JP. Analysis of the Complexity and Diversity of mRNAs from Pollen and Shoots of Tradescantia. Plant Physiol. 1984 Jul;75(3):865–868. [PMC free article] [PubMed]

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