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Plant Physiol. Nov 1994; 106(3): 905–915.
PMCID: PMC159613

Circadian oscillations of a transcript encoding a germin-like protein that is associated with cell walls in young leaves of the long-day plant Sinapis alba L.

Abstract

As part of an attempt to analyze rhythmic phenomena in the long-day plant Sinapis alba L. at the molecular level, we have searched for mRNAs whose concentration varies as a function of time of day. Differential screening of a cDNA library established from mRNAs expressed at the end of the daily light phase with probes representing transcripts expressed predominantly in the morning or evening has identified one major transcript. The cDNA, Saglp, encodes a predicted 22-kD protein with an N-terminal signal sequence. The protein shows homology to germin, a protein expressed in wheat embryos after onset of germination. The Saglp mRNA level undergoes circadian oscillations in light/dark cycles with maxima between 8 and 12 PM (zeitgeber time [zt]12-zt16) and minima around 8 PM (zt0). In plants grown from seed in constant light, transcript levels are constitutive. In constant light regular temperature shifts function as an alternative "zeitgeber" to initiate Saglp transcript oscillations. At the cellular level, Saglp transcripts are expressed in the epidermis and spongy parenchyma of young leaves, and in distinct regions of the epidermis and the cortex in stems and petioles. Strong signals are observed in these tissues around zt12, whereas little expression is found around zt20, suggesting that the underlying oscillatory mechanism(s) operate(s) synchronously in different plant organs. The SaGLP steady-state protein concentration remains constant over light/dark cycles. Immunogold labeling shows that the SaGLP protein is associated with primary cell walls.

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Selected References

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  • Adamska I, Scheel B, Kloppstech K. Circadian oscillations of nuclear-encoded chloroplast proteins in pea (Pisum sativum). Plant Mol Biol. 1991 Nov;17(5):1055–1065. [PubMed]
  • Anderson DJ, Blobel G. Immunoprecipitation of proteins from cell-free translations. Methods Enzymol. 1983;96:111–120. [PubMed]
  • Bernier F, Lemieux G, Pallotta D. Gene families encode the major encystment-specific proteins of Physarum polycephalum plasmodia. Gene. 1987;59(2-3):265–277. [PubMed]
  • Bonner WM, Laskey RA. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. [PubMed]
  • Busheva M, Garab G, Liker E, Tóth Z, Szèll M, Nagy F. Diurnal Fluctuations in the Content and Functional Properties of the Light Harvesting Chlorophyll a/b Complex in Thylakoid Membranes : Correlation with the Diurnal Rhythm of the mRNA Level. Plant Physiol. 1991 Apr;95(4):997–1003. [PMC free article] [PubMed]
  • Cremer F, Dommes J, Van de Walle C, Bernier G. Diurnal Rhythmicity in the Pattern of mRNAs in the Leaves of Sinapis alba. Plant Physiol. 1990 Dec;94(4):1590–1597. [PMC free article] [PubMed]
  • Esen A. A simple method for quantitative, semiquantitative, and qualitative assay of protein. Anal Biochem. 1978 Aug 15;89(1):264–273. [PubMed]
  • Feinberg AP, Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. [PubMed]
  • Giuliano G, Hoffman NE, Ko K, Scolnik PA, Cashmore AR. A light-entrained circadian clock controls transcription of several plant genes. EMBO J. 1988 Dec 1;7(12):3635–3642. [PMC free article] [PubMed]
  • Gorton HL, Williams WE, Binns ME, Gemmell CN, Leheny EA, Shepherd AC. Circadian Stomatal Rhythms in Epidermal Peels from Vicia faba. Plant Physiol. 1989 Aug;90(4):1329–1334. [PMC free article] [PubMed]
  • Heintzen C, Melzer S, Fischer R, Kappeler S, Apel K, Staiger D. A light- and temperature-entrained circadian clock controls expression of transcripts encoding nuclear proteins with homology to RNA-binding proteins in meristematic tissue. Plant J. 1994 Jun;5(6):799–813. [PubMed]
  • Kloppstech K, Otto B, Sierralta W. Cyclic temperature treatments of dark-grown pea seedlings induce a rise in specific transcript levels of light-regulated genes related to photomorphogenesis. Mol Gen Genet. 1991 Mar;225(3):468–473. [PubMed]
  • Kondo T, Strayer CA, Kulkarni RD, Taylor W, Ishiura M, Golden SS, Johnson CH. Circadian rhythms in prokaryotes: luciferase as a reporter of circadian gene expression in cyanobacteria. Proc Natl Acad Sci U S A. 1993 Jun 15;90(12):5672–5676. [PMC free article] [PubMed]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed]
  • Lane BG, Bernier F, Dratewka-Kos E, Shafai R, Kennedy TD, Pyne C, Munro JR, Vaughan T, Walters D, Altomare F. Homologies between members of the germin gene family in hexaploid wheat and similarities between these wheat germins and certain Physarum spherulins. J Biol Chem. 1991 Jun 5;266(16):10461–10469. [PubMed]
  • Lane BG, Cuming AC, Frégeau J, Carpita NC, Hurkman WJ, Bernier F, Dratewka-Kos E, Kennedy TD. Germin isoforms are discrete temporal markers of wheat development. Pseudogermin is a uniquely thermostable water-soluble oligomeric protein in ungerminated embryos and like germin in germinated embryos, it is incorporated into cell walls. Eur J Biochem. 1992 Nov 1;209(3):961–969. [PubMed]
  • Lane BG, Dunwell JM, Ray JA, Schmitt MR, Cuming AC. Germin, a protein marker of early plant development, is an oxalate oxidase. J Biol Chem. 1993 Jun 15;268(17):12239–12242. [PubMed]
  • Leutwiler LS, Meyerowitz EM, Tobin EM. Structure and expression of three light-harvesting chlorophyll a/b-binding protein genes in Arabidopsis thaliana. Nucleic Acids Res. 1986 May 27;14(10):4051–4064. [PMC free article] [PubMed]
  • Melzer S, Majewski DM, Apel K. Early Changes in Gene Expression during the Transition from Vegetative to Generative Growth in the Long-Day Plant Sinapis alba. Plant Cell. 1990 Oct;2(10):953–961. [PMC free article] [PubMed]
  • Millar AJ, Kay SA. Circadian Control of cab Gene Transcription and mRNA Accumulation in Arabidopsis. Plant Cell. 1991 May;3(5):541–550. [PMC free article] [PubMed]
  • Piechulla B, Gruissem W. Diurnal mRNA fluctuations of nuclear and plastid genes in developing tomato fruits. EMBO J. 1987 Dec 1;6(12):3593–3599. [PMC free article] [PubMed]
  • Redinbaugh MG, Sabre M, Scandalios JG. Expression of the maize Cat3 catalase gene is under the influence of a circadian rhythm. Proc Natl Acad Sci U S A. 1990 Sep;87(17):6853–6857. [PMC free article] [PubMed]
  • Satter RL, Geballe GT, Applewhite PB, Galston AW. Potassium flux and leaf movement in Samanea saman. I. Rhythmic movement. J Gen Physiol. 1974 Oct;64(4):413–430. [PMC free article] [PubMed]
  • Staiger D, Kappeler S, Müller M, Apel K. The proteins encoded by two tapetum-specific transcripts, Sa tap35 and Sa tap44, from Sinapis alba L. are localized in the exine cell wall layer of developing microspores. Planta. 1994;192(2):221–231. [PubMed]
  • Studer D, Michel M, Müller M. High pressure freezing comes of age. Scanning Microsc Suppl. 1989;3:253–269. [PubMed]
  • Zheng CC, Bui AQ, O'Neill SD. Abundance of an mRNA encoding a high mobility group DNA-binding protein is regulated by light and an endogenous rhythm. Plant Mol Biol. 1993 Nov;23(4):813–823. [PubMed]

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