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Plant Physiol. Jun 1994; 105(2): 601–605.
PMCID: PMC159399

Genetic Enhancement of Cold Tolerance by Expression of a Gene for Chloroplast [omega]-3 Fatty Acid Desaturase in Transgenic Tobacco.

Abstract

The increased production of trienoic fatty acids, hexadecatrienoic (16:3) and linolenic (18:3) acids, is a response connected with cold acclimation of higher plants and is thought to protect plant cells against cold damage. Transgenic tobacco (Nicotiana tabacum cv SR1) plants that contain increased levels of 16:3 and 18:3 fatty acids, and correspondingly decreased levels of their precursors, hexadecadienoic and linoleic acids, were engineered by introduction of a chloroplast [omega]-3 fatty acid desaturase gene (the fad7 gene) isolated from Arabidopsis thaliana. When exposed to 1[deg]C for 7 d and then cultured at 25[deg]C, the suppression of leaf growth observed in the wild-type plants was significantly alleviated in the transgenic plants with the fad7 gene. The low-temperature- induced chlorosis was also much reduced in the plants transformed with the fad7 gene. These results indicate that increased levels of trienoic fatty acids in genetically engineered plants enhance cold tolerance.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Arondel V, Lemieux B, Hwang I, Gibson S, Goodman HM, Somerville CR. Map-based cloning of a gene controlling omega-3 fatty acid desaturation in Arabidopsis. Science. 1992 Nov 20;258(5086):1353–1355. [PubMed]
  • Browse J, Kunst L, Anderson S, Hugly S, Somerville C. A mutant of Arabidopsis deficient in the chloroplast 16:1/18:1 desaturase. Plant Physiol. 1989 Jun;90(2):522–529. [PMC free article] [PubMed]
  • Browse J, McCourt P, Somerville C. A mutant of Arabidopsis deficient in c(18:3) and c(16:3) leaf lipids. Plant Physiol. 1986 Jul;81(3):859–864. [PMC free article] [PubMed]
  • Hugly S, Somerville C. A role for membrane lipid polyunsaturation in chloroplast biogenesis at low temperature. Plant Physiol. 1992 May;99(1):197–202. [PMC free article] [PubMed]
  • Iba K, Gibson S, Nishiuchi T, Fuse T, Nishimura M, Arondel V, Hugly S, Somerville C. A gene encoding a chloroplast omega-3 fatty acid desaturase complements alterations in fatty acid desaturation and chloroplast copy number of the fad7 mutant of Arabidopsis thaliana. J Biol Chem. 1993 Nov 15;268(32):24099–24105. [PubMed]
  • Los D, Horvath I, Vigh L, Murata N. The temperature-dependent expression of the desaturase gene desA in Synechocystis PCC6803. FEBS Lett. 1993 Feb 22;318(1):57–60. [PubMed]
  • Somerville C, Browse J. Plant lipids: metabolism, mutants, and membranes. Science. 1991 Apr 5;252(5002):80–87. [PubMed]
  • Wada H, Gombos Z, Murata N. Enhancement of chilling tolerance of a cyanobacterium by genetic manipulation of fatty acid desaturation. Nature. 1990 Sep 13;347(6289):200–203. [PubMed]
  • Wolter FP, Schmidt R, Heinz E. Chilling sensitivity of Arabidopsis thaliana with genetically engineered membrane lipids. EMBO J. 1992 Dec;11(13):4685–4692. [PMC free article] [PubMed]
  • Yadav NS, Wierzbicki A, Aegerter M, Caster CS, Pérez-Grau L, Kinney AJ, Hitz WD, Booth JR, Jr, Schweiger B, Stecca KL, et al. Cloning of higher plant omega-3 fatty acid desaturases. Plant Physiol. 1993 Oct;103(2):467–476. [PMC free article] [PubMed]

Articles from Plant Physiology are provided here courtesy of American Society of Plant Biologists

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