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Plant Physiol. May 1994; 105(1): 405–413.
PMCID: PMC159369

Carotenoid Biosynthesis during Tomato Fruit Development (Evidence for Tissue-Specific Gene Expression).

Abstract

Tomato (Lycopersicon esculentum Mill. cv Ailsa Craig) fruit, at five stages of development, have been analyzed for their carotenoid and chlorophyll (Chl) contents, in vitro activities of phytoene synthase, phytoene desaturase, and lycopene cyclase, as well as expression of the phytoene synthase (Psy) and phytoene desaturase (Pds) genes. During ripening, the total carotenoids increased with a concomitant decrease in Chl. Although the highest carotenoid content (consisting mainly of lycopene and [beta]-carotene) was found in ripe fruit, the greatest carotenogenic enzymic activities were found in green fruit. Phytoene synthase was located in the plastid stroma, whereas the metabolism of phytoene was associated with plastid membranes during all stages of fruit development. The in vitro products of phytoene desaturation altered from being predominantly phytofluence and [zeta]-carotene in chloroplasts to becoming mainly lycopene in chromoplasts. The expression of Psy was detected in breaker and ripe fruit, as well as flowers, but was not detectable by northern blot analysis in leaves or green fruits. The Pds gene transcript was barely detectable in green fruit and leaves but was expressed in flowers and breaker fruit. These results suggest that transcription of Psy and Pds is regulated developmentally, with expression being considerably elevated in chromoplast-containing tissues. Antiserum to the Synechococcus phytoene synthase cross-reacted with phytoene synthase of green fruit only on western blots and not with the enzyme from ripe fruit. In contrast, a monoclonal antibody to the Psy gene product only cross-reacted with phytoene synthase from ripe fruit. The enzymes from green and ripe fruit had different molecular masses of 42 and 38 kD, respectively. The absence of detectable Psy and Pds mRNA in green tissues using northern blot analyses, despite high levels of phytoene synthase and desaturase activity, lends support to the hypothesis of divergent genes encoding these enzymes.

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Selected References

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  • Bartley GE, Viitanen PV, Bacot KO, Scolnik PA. A tomato gene expressed during fruit ripening encodes an enzyme of the carotenoid biosynthesis pathway. J Biol Chem. 1992 Mar 15;267(8):5036–5039. [PubMed]
  • Chamovitz D, Misawa N, Sandmann G, Hirschberg J. Molecular cloning and expression in Escherichia coli of a cyanobacterial gene coding for phytoene synthase, a carotenoid biosynthesis enzyme. FEBS Lett. 1992 Jan 27;296(3):305–310. [PubMed]
  • Giuliano G, Bartley GE, Scolnik PA. Regulation of carotenoid biosynthesis during tomato development. Plant Cell. 1993 Apr;5(4):379–387. [PMC free article] [PubMed]
  • Hugueney P, Römer S, Kuntz M, Camara B. Characterization and molecular cloning of a flavoprotein catalyzing the synthesis of phytofluene and zeta-carotene in Capsicum chromoplasts. Eur J Biochem. 1992 Oct 1;209(1):399–407. [PubMed]
  • Kuntz M, Römer S, Suire C, Hugueney P, Weil JH, Schantz R, Camara B. Identification of a cDNA for the plastid-located geranylgeranyl pyrophosphate synthase from Capsicum annuum: correlative increase in enzyme activity and transcript level during fruit ripening. Plant J. 1992 Jan;2(1):25–34. [PubMed]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed]
  • Lawrence SD, Cline K, Moore GA. Chromoplast-Targeted Proteins in Tomato (Lycopersicon esculentum Mill.) Fruit. Plant Physiol. 1993 Jul;102(3):789–794. [PMC free article] [PubMed]
  • Mackinney G, Rick CM, Jenkins JA. THE PHYTOENE CONTENT OF TOMATOES. Proc Natl Acad Sci U S A. 1956 Jul;42(7):404–408. [PMC free article] [PubMed]
  • Maudinas B, Bucholtz ML, Papastephanou C, Katiyar SS, Briedis AV, Porter JW. Adenosine 5'-triphosphate stimulation of the activity of a partially purified phytoene synthetase complex. Biochem Biophys Res Commun. 1975 Sep 2;66(1):430–436. [PubMed]
  • Narita JO, Gruissem W. Tomato hydroxymethylglutaryl-CoA reductase is required early in fruit development but not during ripening. Plant Cell. 1989 Feb;1(2):181–190. [PMC free article] [PubMed]
  • Park H, Denbow CJ, Cramer CL. Structure and nucleotide sequence of tomato HMG2 encoding 3-hydroxy-3-methyl-glutaryl coenzyme A reductase. Plant Mol Biol. 1992 Oct;20(2):327–331. [PubMed]
  • Piechulla B, Glick RE, Bahl H, Melis A, Gruissem W. Changes in Photosynthetic Capacity and Photosynthetic Protein Pattern during Tomato Fruit Ripening. Plant Physiol. 1987 Jul;84(3):911–917. [PMC free article] [PubMed]
  • Porter JW. Enzymatic synthesis of carotenes and related compounds. Pure Appl Chem. 1969;20(4):449–481. [PubMed]
  • PORTER JW, LINCOLN RE. Lycopersicon selections containing a high content of carotenes and colorless polyenes; the mechanism of carotene biosynthesis. Arch Biochem. 1950 Jul;27(2):390–403. [PubMed]
  • Premachandra BR. Genetic regulation of carotene biosynthesis in selected tomato strains: aspects of beta-carotene biosynthesis and B gene specificity. Int J Vitam Nutr Res. 1986;56(1):35–43. [PubMed]
  • Smith CJ, Watson CF, Morris PC, Bird CR, Seymour GB, Gray JE, Arnold C, Tucker GA, Schuch W, Harding S, et al. Inheritance and effect on ripening of antisense polygalacturonase genes in transgenic tomatoes. Plant Mol Biol. 1990 Mar;14(3):369–379. [PubMed]

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