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Plant Physiol. Feb 1994; 104(2): 591–596.
PMCID: PMC159235

A small GTP-binding protein from Arabidopsis thaliana functionally complements the yeast YPT6 null mutant.


A clone designated A.t.RAB6 encoding a small GTP-binding protein was isolated from a cDNA library of Arabidopsis thaliana leaf tissue. The predicted amino acid sequence was highly homologous to the mammalian and yeast counterparts, H.Rab6 and Ryh1/Ypt6, respectively. Lesser homology was found between the predicted Arabidopsis protein sequence and two small GTP-binding proteins isolated from plant species (44% homology to Zea mays Ypt1 and 43% homology to Nicotiana tabacum Rab5). Conserved stretches in the deduced amino acid sequence of A.t.Rab6 include four regions involved in GTP-binding, an effector region, and C-terminal cysteine residues required for prenylation and subsequent membrane attachment. Northern blot analysis demonstrated that A.t.Rab6 mRNA was expressed in root, leaf, stem, and flower tissues from A. thaliana with the highest levels present in roots. Escherichia coli produced histidine-tagged A.t.Rab6 protein-bound GTP, whereas a mutation in one of the guanine nucleotide-binding sites (asparagine122 to isoleucine) rendered it incapable of binding GTP. Functionally, the A.t.RAB6 gene was able to complement the temperature-sensitive phenotype of the YPT6 null mutant in yeast. The isolation of this gene will aid in the dissection of the machinery involved in soluble protein sorting at the trans-Golgi network of plants.

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Selected References

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  • Antony C, Cibert C, Géraud G, Santa Maria A, Maro B, Mayau V, Goud B. The small GTP-binding protein rab6p is distributed from medial Golgi to the trans-Golgi network as determined by a confocal microscopic approach. J Cell Sci. 1992 Nov;103(Pt 3):785–796. [PubMed]
  • Balch WE. Small GTP-binding proteins in vesicular transport. Trends Biochem Sci. 1990 Dec;15(12):473–477. [PubMed]
  • Barbacid M. ras genes. Annu Rev Biochem. 1987;56:779–827. [PubMed]
  • Barlowe C, d'Enfert C, Schekman R. Purification and characterization of SAR1p, a small GTP-binding protein required for transport vesicle formation from the endoplasmic reticulum. J Biol Chem. 1993 Jan 15;268(2):873–879. [PubMed]
  • Bednarek SY, Raikhel NV. Intracellular trafficking of secretory proteins. Plant Mol Biol. 1992 Oct;20(1):133–150. [PubMed]
  • Chavrier P, Gorvel JP, Stelzer E, Simons K, Gruenberg J, Zerial M. Hypervariable C-terminal domain of rab proteins acts as a targeting signal. Nature. 1991 Oct 24;353(6346):769–772. [PubMed]
  • Chrispeels MJ, Raikhel NV. Short peptide domains target proteins to plant vacuoles. Cell. 1992 Feb 21;68(4):613–616. [PubMed]
  • Dallmann G, Sticher L, Marshallsay C, Nagy F. Molecular characterization of tobacco cDNAs encoding two small GTP-binding proteins. Plant Mol Biol. 1992 Aug;19(5):847–857. [PubMed]
  • Dombrowski JE, Schroeder MR, Bednarek SY, Raikhel NV. Determination of the functional elements within the vacuolar targeting signal of barley lectin. Plant Cell. 1993 May;5(5):587–596. [PMC free article] [PubMed]
  • Goud B, Zahraoui A, Tavitian A, Saraste J. Small GTP-binding protein associated with Golgi cisternae. Nature. 1990 Jun 7;345(6275):553–556. [PubMed]
  • Haubruck H, Prange R, Vorgias C, Gallwitz D. The ras-related mouse ypt1 protein can functionally replace the YPT1 gene product in yeast. EMBO J. 1989 May;8(5):1427–1432. [PMC free article] [PubMed]
  • Hengst L, Lehmeier T, Gallwitz D. The ryh1 gene in the fission yeast Schizosaccharomyces pombe encoding a GTP-binding protein related to ras, rho and ypt: structure, expression and identification of its human homologue. EMBO J. 1990 Jun;9(6):1949–1955. [PMC free article] [PubMed]
  • Ito H, Fukuda Y, Murata K, Kimura A. Transformation of intact yeast cells treated with alkali cations. J Bacteriol. 1983 Jan;153(1):163–168. [PMC free article] [PubMed]
  • Kunkel TA, Roberts JD, Zakour RA. Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods Enzymol. 1987;154:367–382. [PubMed]
  • Lawson SG, Mason TL, Sabin RD, Sloan ME, Drake RR, Haley BE, Wasserman BP. UDP-Glucose: (1,3)-beta-Glucan Synthase from Daucus carota L. : Characterization, Photoaffinity Labeling, and Solubilization. Plant Physiol. 1989 May;90(1):101–108. [PMC free article] [PubMed]
  • Lerner DR, Raikhel NV. The gene for stinging nettle lectin (Urtica dioica agglutinin) encodes both a lectin and a chitinase. J Biol Chem. 1992 Jun 5;267(16):11085–11091. [PubMed]
  • Pai EF, Kabsch W, Krengel U, Holmes KC, John J, Wittinghofer A. Structure of the guanine-nucleotide-binding domain of the Ha-ras oncogene product p21 in the triphosphate conformation. Nature. 1989 Sep 21;341(6239):209–214. [PubMed]
  • Palme K, Diefenthal T, Vingron M, Sander C, Schell J. Molecular cloning and structural analysis of genes from Zea mays (L.) coding for members of the ras-related ypt gene family. Proc Natl Acad Sci U S A. 1992 Jan 15;89(2):787–791. [PMC free article] [PubMed]
  • Pryer NK, Wuestehube LJ, Schekman R. Vesicle-mediated protein sorting. Annu Rev Biochem. 1992;61:471–516. [PubMed]
  • Strom M, Vollmer P, Tan TJ, Gallwitz D. A yeast GTPase-activating protein that interacts specifically with a member of the Ypt/Rab family. Nature. 1993 Feb 25;361(6414):736–739. [PubMed]
  • Terryn N, Van Montagu M, Inzé D. GTP-binding proteins in plants. Plant Mol Biol. 1993 Apr;22(1):143–152. [PubMed]
  • Tisdale EJ, Bourne JR, Khosravi-Far R, Der CJ, Balch WE. GTP-binding mutants of rab1 and rab2 are potent inhibitors of vesicular transport from the endoplasmic reticulum to the Golgi complex. J Cell Biol. 1992 Nov;119(4):749–761. [PMC free article] [PubMed]
  • Vieira J, Messing J. Production of single-stranded plasmid DNA. Methods Enzymol. 1987;153:3–11. [PubMed]
  • Wagner P, Hengst L, Gallwitz D. Ypt proteins in yeast. Methods Enzymol. 1992;219:369–387. [PubMed]
  • Schwarzbauer JE, Patel RS, Fonda D, Hynes RO. Multiple sites of alternative splicing of the rat fibronectin gene transcript. EMBO J. 1987 Sep;6(9):2573–2580. [PMC free article] [PubMed]
  • Walworth NC, Goud B, Kabcenell AK, Novick PJ. Mutational analysis of SEC4 suggests a cyclical mechanism for the regulation of vesicular traffic. EMBO J. 1989 Jun;8(6):1685–1693. [PMC free article] [PubMed]

Articles from Plant Physiology are provided here courtesy of American Society of Plant Biologists


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