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Plant Physiol. Aug 1993; 102(4): 1269–1273.
PMCID: PMC158914

Differentiation of the Properties of the Branching Isozymes from Maize (Zea mays).

Abstract

The multiple forms of branching enzyme (BE) from developing maize (Zea mays) endosperm were purified by modification of previous procedures such that amylase activity could be eliminated completely from the BE preparation. Three distinct assays for BE activity (phosphorylase a stimulation assay, BE linkage assay, and iodine stain assay) were used to characterize and differentiate the properties of the BE isoforms. This study presents the first evidence that the BE isoforms differ in their action on amylopectin. BEI had the highest activity in branching amylose, but its rate of branching amylopectin was less than 5% of that of branching amylose. Conversely, BEII isoforms had lower rates in branching amylose (about 9-12% of that of BEI) and had higher rates of branching amylopectin (about 6-fold) than BEI. The implication of these findings to the mechanism of amylopectin synthesis in vivo are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Borovsky D, Smith EE, Whelan WJ. Purification and properties of potato 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-alpha-(1,4-alpha-glucano)-transferase. Evidence against a dual catalytic function in amylose-branching enzyme. Eur J Biochem. 1975 Nov 15;59(2):615–625. [PubMed]
  • Boyer CD, Preiss J. Multiple forms of starch branching enzyme of maize: evidence for independent genetic control. Biochem Biophys Res Commun. 1978 Jan 13;80(1):169–175. [PubMed]
  • Fisher MB, Boyer CD. Immunological characterization of maize starch branching enzymes. Plant Physiol. 1983 Jul;72(3):813–816. [PMC free article] [PubMed]
  • Hedman KD, Boyer CD. Gene dosage at the amylose-extender locus of maize: effects on the levels of starch branching enzymes. Biochem Genet. 1982 Jun;20(5-6):483–492. [PubMed]
  • Okita TW. Is there an alternative pathway for starch synthesis? Plant Physiol. 1992 Oct;100(2):560–564. [PMC free article] [PubMed]
  • Singh BK, Preiss J. Starch Branching Enzymes from Maize : Immunological Characterization using Polyclonal and Monoclonal Antibodies. Plant Physiol. 1985 Sep;79(1):34–40. [PMC free article] [PubMed]
  • Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ, Klenk DC. Measurement of protein using bicinchoninic acid. Anal Biochem. 1985 Oct;150(1):76–85. [PubMed]
  • Vos-Scheperkeuter GH, de Wit JG, Ponstein AS, Feenstra WJ, Witholt B. Immunological comparison of the starch branching enzymes from potato tubers and maize kernels. Plant Physiol. 1989 May;90(1):75–84. [PMC free article] [PubMed]

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