• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of plntphysLink to Publisher's site
Plant Physiol. Jul 1997; 114(3): 957–968.
PMCID: PMC158384

Differential effect of purified spruce chitinases and beta-1,3-glucanases on the activity of elicitors from ectomycorrhizal fungi.

Abstract

Two chitinases (EC 3.2.1.14) and two beta-1,3-glucanases (EC 3.2.1.39) were purified from the culture medium of spruce (Picea abines [L.] Karst.) cells to study their role in modifying elicitors, cell walls, growth, and hyphal morphology of ectomycorrhizal fungi. The 36-kD class I chitinase (isoelectric point [pl] 8.0) and the 28-kD chitinase (pl 8.7) decreased the activity of elicitor preparations from Hebeloma crustuliniforme (Bull. ex Fries.) Quél., Amanita muscaria (L.) Pers., and Suillus variegatus (Sw.: Fr.) O.K., as demonstrated by using the elicitor-induced extracellular alkalinization in spruce cells as a test system. In addition, chitinases released monomeric products from the walls of these ectomycorrhizal fungi. The beta-1,3-glucanases (35 kD, pl 3.7 and 3.9), in contrast, had little influence on the activity of the fungal elicitors and released only from walls of A. muscaria some polymeric products. Furthermore, chitinases alone and in combination with beta-1,3-glucanases had no effect on the growth and morphology of the hyphae. Thus, it is suggested that apoplastic chitinases in the root cortex destroy elicitors from the ectomycorrhizal fungi without damaging the fungus. By this mechanism the host plant could attenuate the elicitor signal and adjust its own defense reactions to a level allowing symbiotic interaction.

Full Text

The Full Text of this article is available as a PDF (2.7M).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Kohjiro M, Koga K, Komori M, Fukutome T, Yasaka B, Urakami H. [A case report of a burn produced by the probe of a pulse oximeter]. Masui. 1992 Dec;41(12):1991–1993. [PubMed]
  • Ansorge W. Fast and sensitive detection of protein and DNA bands by treatment with potassium permanganate. J Biochem Biophys Methods. 1985 May;11(1):13–20. [PubMed]
  • Atkinson MM, Keppler LD, Orlandi EW, Baker CJ, Mischke CF. Involvement of plasma membrane calcium influx in bacterial induction of the k/h and hypersensitive responses in tobacco. Plant Physiol. 1990 Jan;92(1):215–221. [PMC free article] [PubMed]
  • Atkinson MM, Midland SL, Sims JJ, Keen NT. Syringolide 1 Triggers Ca2+ Influx, K+ Efflux, and Extracellular Alkalization in Soybean Cells Carrying the Disease-Resistance Gene Rpg4. Plant Physiol. 1996 Sep;112(1):297–302. [PMC free article] [PubMed]
  • Baureithel K, Felix G, Boller T. Specific, high affinity binding of chitin fragments to tomato cells and membranes. Competitive inhibition of binding by derivatives of chitooligosaccharides and a Nod factor of Rhizobium. J Biol Chem. 1994 Jul 8;269(27):17931–17938. [PubMed]
  • Brogue K, Chet I, Holliday M, Cressman R, Biddle P, Knowlton S, Mauvais CJ, Broglie R. Transgenic Plants with Enhanced Resistance to the Fungal Pathogen Rhizoctonia solani. Science. 1991 Nov 22;254(5035):1194–1197. [PubMed]
  • Brownleader MD, Ahmed N, Trevan M, Chaplin MF, Dey PM. Purification and Partial Characterization of Tomato Extensin Peroxidase. Plant Physiol. 1995 Nov;109(3):1115–1123. [PMC free article] [PubMed]
  • Conrath U, Jeblick W, Kauss H. The protein kinase inhibitor, K-252a, decreases elicitor-induced Ca2+ uptake and K+ release, and increases coumarin synthesis in parsley cells. FEBS Lett. 1991 Feb 11;279(1):141–144. [PubMed]
  • Kirsch C, Hahlbrock K, Kombrink E. Purification and characterization of extracellular, acidic chitinase isoenzymes from elicitor-stimulated parsley cells. Eur J Biochem. 1993 Apr 1;213(1):419–425. [PubMed]
  • Mauch F, Mauch-Mani B, Boller T. Antifungal Hydrolases in Pea Tissue : II. Inhibition of Fungal Growth by Combinations of Chitinase and beta-1,3-Glucanase. Plant Physiol. 1988 Nov;88(3):936–942. [PMC free article] [PubMed]
  • Molano J, Durán A, Cabib E. A rapid and sensitive assay for chitinase using tritiated chitin. Anal Biochem. 1977 Dec;83(2):648–656. [PubMed]
  • Molano J, Polacheck I, Duran A, Cabib E. An endochitinase from wheat germ. Activity on nascent and preformed chitin. J Biol Chem. 1979 Jun 10;254(11):4901–4907. [PubMed]
  • O'Farrell PH. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975 May 25;250(10):4007–4021. [PMC free article] [PubMed]
  • Okinaka Y, Mimori K, Takeo K, Kitamura S, Takeuchi Y, Yamaoka N, Yoshikawa M. A structural model for the mechanisms of elicitor release from fungal cell walls by plant beta-1,3-endoglucanase. Plant Physiol. 1995 Nov;109(3):839–845. [PMC free article] [PubMed]
  • Regalado AP, Ricardo CP. Study of the intercellular fluid of healthy Lupinus albus organs. Presence of a chitinase and a thaumatin-like protein. Plant Physiol. 1996 Jan;110(1):227–232. [PMC free article] [PubMed]
  • Sharp JK, McNeil M, Albersheim P. The primary structures of one elicitor-active and seven elicitor-inactive hexa(beta-D-glucopyranosyl)-D-glucitols isolated from the mycelial walls of Phytophthora megasperma f. sp. glycinea. J Biol Chem. 1984 Sep 25;259(18):11321–11336. [PubMed]
  • Shinshi H, Mohnen D, Meins F. Regulation of a plant pathogenesis-related enzyme: Inhibition of chitinase and chitinase mRNA accumulation in cultured tobacco tissues by auxin and cytokinin. Proc Natl Acad Sci U S A. 1987 Jan;84(1):89–93. [PMC free article] [PubMed]
  • Staehelin C, Granado J, Müller J, Wiemken A, Mellor RB, Felix G, Regenass M, Broughton WJ, Boller T. Perception of Rhizobium nodulation factors by tomato cells and inactivation by root chitinases. Proc Natl Acad Sci U S A. 1994 Mar 15;91(6):2196–2200. [PMC free article] [PubMed]
  • Trudel J, Asselin A. Detection of chitinase activity after polyacrylamide gel electrophoresis. Anal Biochem. 1989 May 1;178(2):362–366. [PubMed]
  • Viard MP, Martin F, Pugin A, Ricci P, Blein JP. Protein Phosphorylation Is Induced in Tobacco Cells by the Elicitor Cryptogein. Plant Physiol. 1994 Apr;104(4):1245–1249. [PMC free article] [PubMed]
  • Vogelsang R, Barz W. Purification, characterization and differential hormonal regulation of a beta-1,3-glucanase and two chitinases from chickpea (Cicer arietinum L.). Planta. 1993 Jan;189(1):60–69. [PubMed]

Articles from Plant Physiology are provided here courtesy of American Society of Plant Biologists

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...