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Plant Physiol. Jul 1997; 114(3): 927–935.
PMCID: PMC158381

Antisense expression of the peptide transport gene AtPTR2-B delays flowering and arrests seed development in transgenic Arabidopsis plants.

Abstract

Previously, we identified a peptide transport gene, AtPTR2-B, from Arabidopsis thaliana that was constitutively expressed in all plant organs, suggesting an important physiological role in plant growth and development. To evaluate the function of this transporter, transgenic Arabidopsis plants were constructed expressing antisense or sense AtPTR2-B. Genomic Southern analysis indicated that four independent antisense and three independent sense AtPTR2-B transgenic lines were obtained, which was confirmed by analysis of the segregation of the kanamycin resistance gene carried on the T-DNA. RNA blot data showed that the endogenous AtPTR2-B mRNA levels were significantly reduced in transgenic leaves and flowers, but not in transgenic roots. Consistent with this reduction in endogenous AtPTR2-B mRNA levels, all four antisense lines and one sense line exhibited significant phenotypic changes, including late flowering and arrested seed development. These phenotypic changes could be explained by a defect in nitrogen nutrition due to the reduced peptide transport activity conferred by AtPTR2-B. These results suggest that AtPTR2-B may play a general role in plant nutrition. The AtPTR2-B gene was mapped to chromosome 2, which is closely linked to the restriction fragment length polymorphism marker m246.

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Selected References

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  • Andreae WA, Good NE. The Formation of Indoleacetylaspartic Acid in Pea Seedlings. Plant Physiol. 1955 Jul;30(4):380–382. [PMC free article] [PubMed]
  • Brandsch M, Miyamoto Y, Ganapathy V, Leibach FH. Expression and protein kinase C-dependent regulation of peptide/H+ co-transport system in the Caco-2 human colon carcinoma cell line. Biochem J. 1994 Apr 1;299(Pt 1):253–260. [PMC free article] [PubMed]
  • Castle LA, Errampalli D, Atherton TL, Franzmann LH, Yoon ES, Meinke DW. Genetic and molecular characterization of embryonic mutants identified following seed transformation in Arabidopsis. Mol Gen Genet. 1993 Dec;241(5-6):504–514. [PubMed]
  • Dougherty WG, Parks TD. Transgenes and gene suppression: telling us something new? Curr Opin Cell Biol. 1995 Jun;7(3):399–405. [PubMed]
  • Jamai A, Chollet JF, Delrot S. Proton-Peptide Co-Transport in Broad Bean Leaf Tissues. Plant Physiol. 1994 Nov;106(3):1023–1031. [PMC free article] [PubMed]
  • Koornneef M, Hanhart CJ, van der Veen JH. A genetic and physiological analysis of late flowering mutants in Arabidopsis thaliana. Mol Gen Genet. 1991 Sep;229(1):57–66. [PubMed]
  • Manson MD, Blank V, Brade G, Higgins CF. Peptide chemotaxis in E. coli involves the Tap signal transducer and the dipeptide permease. Nature. 1986 May 15;321(6067):253–256. [PubMed]
  • Mathiopoulos C, Mueller JP, Slack FJ, Murphy CG, Patankar S, Bukusoglu G, Sonenshein AL. A Bacillus subtilis dipeptide transport system expressed early during sporulation. Mol Microbiol. 1991 Aug;5(8):1903–1913. [PubMed]
  • Mitchell RE, Bieleski RL. Involvement of phaseolotoxin in halo blight of beans: transport and conversion to functional toxin. Plant Physiol. 1977 Nov;60(5):723–729. [PMC free article] [PubMed]
  • Payne JW, Smith MW. Peptide transport by micro-organisms. Adv Microb Physiol. 1994;36:1–80. [PubMed]
  • Salmenkallio M, Sopanen T. Amino Acid and Peptide uptake in the scutella of germinating grains of barley, wheat, rice, and maize. Plant Physiol. 1989 Apr;89(4):1285–1291. [PMC free article] [PubMed]
  • Song W, Steiner HY, Zhang L, Naider F, Stacey G, Becker JM. Cloning of a second Arabidopsis peptide transport gene. Plant Physiol. 1996 Jan;110(1):171–178. [PMC free article] [PubMed]
  • Sopanen T, Burston D, Matthews DM. Uptake of small peptides by the scutellum of germinating barley. FEBS Lett. 1977 Jul 1;79(1):4–7. [PubMed]
  • Staskawicz BJ, Panopoulos NJ. Phaseolotoxin transport in Escherichia coli and Salmonella typhimurium via the oligopeptide permease. J Bacteriol. 1980 May;142(2):474–479. [PMC free article] [PubMed]
  • Steiner HY, Song W, Zhang L, Naider F, Becker JM, Stacey G. An Arabidopsis peptide transporter is a member of a new class of membrane transport proteins. Plant Cell. 1994 Sep;6(9):1289–1299. [PMC free article] [PubMed]
  • Takaiwa F, Oono K, Sugiura M. The complete nucleotide sequence of a rice 17S rRNA gene. Nucleic Acids Res. 1984 Jul 11;12(13):5441–5448. [PMC free article] [PubMed]
  • Winter A, Thimann KV. Bound indoleacetic Acid in Avena coleoptiles. Plant Physiol. 1966 Feb;41(2):335–342. [PMC free article] [PubMed]

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