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Plant Physiol. Mar 1997; 113(3): 933–942.
PMCID: PMC158213

Accumulation of ricinoleic, lesquerolic, and densipolic acids in seeds of transgenic Arabidopsis plants that express a fatty acyl hydroxylase cDNA from castor bean.

Abstract

A cDNA encoding the oleate 12-hydroxylase from castor bean (Ricinus communis L.) has previously been shown to direct the synthesis of small amounts of ricinoleic acid (12-hydroxyoctadec-cis-9-enoic acid) in seeds of transgenic tobacco plants. Expression of the cDNA under control of the Brassica napus napin promoter in transgenic Arabidopsis thaliana plants resulted in the accumulation of up to 17% of seed fatty acids as ricinoleate and two novel fatty acids that have been identified by gas chromatography-mass spectrometry as lesquerolic (14-hydroxyeicos-cis-11-enoic acid) and densipolic (12-hydroxyoctadec-cis-9,15-dienoic acid) acids. Traces of auricolic acid were also observed. These results suggest that either the castor hydroxylase can utilize oleic acid and eicosenoic acid as substrates for ricinoleic and lesquerolic acid biosynthesis, respectively, or Arabidopsis contains an elongase that accepts ricinoleic acid as a substrate. These observations are also consistent with indirect biochemical evidence that an n-3 desaturase is capable of converting ricinoleic acid to densipolic acid. Expression of the castor hydroxylase also caused enhanced accumulation of oleic acid and a corresponding decrease in the levels of polyunsaturated fatty acids. Since the steady-state level of mRNA for the oleate-12 desaturase was not affected, it appears that the presence of the hydroxylase, directly or indirectly, causes posttranscriptional inhibition of desaturation.

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Selected References

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  • Bafor M, Smith MA, Jonsson L, Stobart K, Stymne S. Ricinoleic acid biosynthesis and triacylglycerol assembly in microsomal preparations from developing castor-bean (Ricinus communis) endosperm. Biochem J. 1991 Dec 1;280(Pt 2):507–514. [PMC free article] [PubMed]
  • Benning C, Somerville CR. Isolation and genetic complementation of a sulfolipid-deficient mutant of Rhodobacter sphaeroides. J Bacteriol. 1992 Apr;174(7):2352–2360. [PMC free article] [PubMed]
  • Browse J, Kunst L, Anderson S, Hugly S, Somerville C. A mutant of Arabidopsis deficient in the chloroplast 16:1/18:1 desaturase. Plant Physiol. 1989 Jun;90(2):522–529. [PMC free article] [PubMed]
  • Abraham PR, Mulder A, Van 't Riet J, Planta RJ, Raué HA. Molecular cloning and physical analysis of an 8.2 kb segment of chromosome XI of Saccharomyces cerevisiae reveals five tightly linked genes. Yeast. 1992 Mar;8(3):227–238. [PubMed]
  • Gibson S, Arondel V, Iba K, Somerville C. Cloning of a temperature-regulated gene encoding a chloroplast omega-3 desaturase from Arabidopsis thaliana. Plant Physiol. 1994 Dec;106(4):1615–1621. [PMC free article] [PubMed]
  • Jones A, Davies HM, Voelker TA. Palmitoyl-acyl carrier protein (ACP) thioesterase and the evolutionary origin of plant acyl-ACP thioesterases. Plant Cell. 1995 Mar;7(3):359–371. [PMC free article] [PubMed]
  • Miquel M, Browse J. Arabidopsis mutants deficient in polyunsaturated fatty acid synthesis. Biochemical and genetic characterization of a plant oleoyl-phosphatidylcholine desaturase. J Biol Chem. 1992 Jan 25;267(3):1502–1509. [PubMed]
  • Somerville C, Browse J. Dissecting desaturation: plants prove advantageous. Trends Cell Biol. 1996 Apr;6(4):148–153. [PubMed]
  • Stahl U, Banas A, Stymne S. Plant Microsomal Phospholipid Acyl Hydrolases Have Selectivities for Uncommon Fatty Acids. Plant Physiol. 1995 Mar;107(3):953–962. [PMC free article] [PubMed]
  • van de Loo FJ, Broun P, Turner S, Somerville C. An oleate 12-hydroxylase from Ricinus communis L. is a fatty acyl desaturase homolog. Proc Natl Acad Sci U S A. 1995 Jul 18;92(15):6743–6747. [PMC free article] [PubMed]

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