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Plant Physiol. Sep 1996; 112(1): 353–359.
PMCID: PMC157956

Molecular cloning of a metallothionein-like gene from Nicotiana glutinosa L. and its induction by wounding and tobacco mosaic virus infection.

Abstract

The cloning and characterization of genes expressed in plant disease resistance could be an initial step toward understanding the molecular mechanisms of disease resistance. A metallothionein-like gene that is inducible by tobacco mosaic virus and by wounding was cloned in the process of subtractive cloning of disease resistance-response genes in Nicotiana glutinosa. One 530-bp cDNA clone (KC9-10) containing an open reading frame of 81 amino acids was characterized. Genomic Southern blot hybridization with the cDNA probe revealed that tobacco metallothionein-like genes are present in few or in one copy per diploid genome. Northern blot hybridization detected strong induction of a 0.5-kb mRNA by wounding and tobacco mosaic virus infection, but only mild induction was detected when copper was tested as an inducer. Methyl jasmonate, salicylic acid, and ethylene were also tested as possible inducers of this gene, but they had no effect on its expression. The possible role of this gene in wounded and pathogen-stressed plants is discussed.

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Selected References

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  • Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol. 1990 Oct 5;215(3):403–410. [PubMed]
  • Buchanan-Wollaston V. Isolation of cDNA clones for genes that are expressed during leaf senescence in Brassica napus. Identification of a gene encoding a senescence-specific metallothionein-like protein. Plant Physiol. 1994 Jul;105(3):839–846. [PMC free article] [PubMed]
  • Choi D, Ward BL, Bostock RM. Differential induction and suppression of potato 3-hydroxy-3-methylglutaryl coenzyme A reductase genes in response to Phytophthora infestans and to its elicitor arachidonic acid. Plant Cell. 1992 Oct;4(10):1333–1344. [PMC free article] [PubMed]
  • Coupe SA, Taylor JE, Roberts JA. Characterisation of an mRNA encoding a metallothionein-like protein that accumulates during ethylene-promoted abscission of Sambucus nigra L. leaflets. Planta. 1995;197(3):442–447. [PubMed]
  • Creelman RA, Tierney ML, Mullet JE. Jasmonic acid/methyl jasmonate accumulate in wounded soybean hypocotyls and modulate wound gene expression. Proc Natl Acad Sci U S A. 1992 Jun 1;89(11):4938–4941. [PMC free article] [PubMed]
  • de Framond AJ. A metallothionein-like gene from maize (Zea mays). Cloning and characterization. FEBS Lett. 1991 Sep 23;290(1-2):103–106. [PubMed]
  • de Miranda JR, Thomas MA, Thurman DA, Tomsett AB. Metallothionein genes from the flowering plant Mimulus guttatus. FEBS Lett. 1990 Jan 29;260(2):277–280. [PubMed]
  • Elmayan T, Tepfer M. Synthesis of a bifunctional metallothionein/beta-glucuronidase fusion protein in transgenic tobacco plants as a means of reducing leaf cadmium levels. Plant J. 1994 Sep;6(3):433–440. [PubMed]
  • Evans IM, Gatehouse LN, Gatehouse JA, Robinson NJ, Croy RR. A gene from pea (Pisum sativum L.) with homology to metallothionein genes. FEBS Lett. 1990 Mar 12;262(1):29–32. [PubMed]
  • Farmer EE, Ryan CA. Octadecanoid Precursors of Jasmonic Acid Activate the Synthesis of Wound-Inducible Proteinase Inhibitors. Plant Cell. 1992 Feb;4(2):129–134. [PMC free article] [PubMed]
  • Fürst P, Hu S, Hackett R, Hamer D. Copper activates metallothionein gene transcription by altering the conformation of a specific DNA binding protein. Cell. 1988 Nov 18;55(4):705–717. [PubMed]
  • Hamer DH. Metallothionein. Annu Rev Biochem. 1986;55:913–951. [PubMed]
  • Mett VL, Lochhead LP, Reynolds PH. Copper-controllable gene expression system for whole plants. Proc Natl Acad Sci U S A. 1993 May 15;90(10):4567–4571. [PMC free article] [PubMed]
  • Pan A, Yang M, Tie F, Li L, Chen Z, Ru B. Expression of mouse metallothionein-I gene confers cadmium resistance in transgenic tobacco plants. Plant Mol Biol. 1994 Jan;24(2):341–351. [PubMed]
  • Parish JH, Kirby KS. Reagents which reduce interactions between ribosomal RNA and rapidly labelled RNA from rat liver. Biochim Biophys Acta. 1966 Dec 21;129(3):554–562. [PubMed]
  • Robinson NJ, Tommey AM, Kuske C, Jackson PJ. Plant metallothioneins. Biochem J. 1993 Oct 1;295(Pt 1):1–10. [PMC free article] [PubMed]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PMC free article] [PubMed]
  • Sive HL, St John T. A simple subtractive hybridization technique employing photoactivatable biotin and phenol extraction. Nucleic Acids Res. 1988 Nov 25;16(22):10937–10937. [PMC free article] [PubMed]
  • Ward ER, Uknes SJ, Williams SC, Dincher SS, Wiederhold DL, Alexander DC, Ahl-Goy P, Metraux JP, Ryals JA. Coordinate Gene Activity in Response to Agents That Induce Systemic Acquired Resistance. Plant Cell. 1991 Oct;3(10):1085–1094. [PMC free article] [PubMed]
  • Yeargan R, Maiti IB, Nielsen MT, Hunt AG, Wagner GJ. Tissue partitioning of cadmium in transgenic tobacco seedlings and field grown plants expressing the mouse metallothionein I gene. Transgenic Res. 1992 Nov;1(6):261–267. [PubMed]
  • Zhou J, Goldsbrough PB. Functional homologs of fungal metallothionein genes from Arabidopsis. Plant Cell. 1994 Jun;6(6):875–884. [PMC free article] [PubMed]
  • Zhou J, Goldsbrough PB. Structure, organization and expression of the metallothionein gene family in Arabidopsis. Mol Gen Genet. 1995 Aug 21;248(3):318–328. [PubMed]

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