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Plant Physiol. Jul 1996; 111(3): 671–678.
PMCID: PMC157882

A brassinosteroid-insensitive mutant in Arabidopsis thaliana exhibits multiple defects in growth and development.

Abstract

Brassinosteroids are widely distributed plant compounds that modulate cell elongation and division, but little is known about the mechanism of action of these plant growth regulators. To investigate brassinosteroids as signals influencing plant growth and development, we identified a brassinosteroid-insensitive mutant in Arabidopsis thaliana (L.) Henyh. ecotype Columbia. The mutant, termed bri1, did not respond to brassinosteroids in hypocotyl elongation and primary root inhibition assays, but it did retain sensitivity to auxins, cytokinins, ethylene, abscisic acid, and gibberellins. The bri1 mutant showed multiple deficiencies in developmental pathways that could not be rescued by brassinosteroid treatment including a severely dwarfed stature; dark green, thickened leaves; males sterility; reduced apical dominance; and de-etiolation of dark-grown seedlings. Genetic analysis suggests that the Bri1 phenotype is caused by a recessive mutation in a single gene with pleiotropic effects that maps 1.6 centimorgans from the cleaved, amplified, polymorphic sequence marker DHS1 on the bottom of chromosome IV. The multiple and dramatic effects of mutation of the BRI1 locus on development suggests that the BRI1 gene may play a critical role in brassinosteroid perception or signal transduction.

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Selected References

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  • Beato M, Herrlich P, Schütz G. Steroid hormone receptors: many actors in search of a plot. Cell. 1995 Dec 15;83(6):851–857. [PubMed]
  • Bell CJ, Ecker JR. Assignment of 30 microsatellite loci to the linkage map of Arabidopsis. Genomics. 1994 Jan 1;19(1):137–144. [PubMed]
  • Bleecker AB, Estelle MA, Somerville C, Kende H. Insensitivity to Ethylene Conferred by a Dominant Mutation in Arabidopsis thaliana. Science. 1988 Aug 26;241(4869):1086–1089. [PubMed]
  • Chang C, Kwok SF, Bleecker AB, Meyerowitz EM. Arabidopsis ethylene-response gene ETR1: similarity of product to two-component regulators. Science. 1993 Oct 22;262(5133):539–544. [PubMed]
  • Clouse SD, Zurek DM, McMorris TC, Baker ME. Effect of brassinolide on gene expression in elongating soybean epicotyls. Plant Physiol. 1992 Nov;100(3):1377–1383. [PMC free article] [PubMed]
  • Daniel SG, Rayle DL, Cleland RE. Auxin physiology of the tomato mutant diageotropica. Plant Physiol. 1989;91:804–807. [PMC free article] [PubMed]
  • Ecker JR. The ethylene signal transduction pathway in plants. Science. 1995 May 5;268(5211):667–675. [PubMed]
  • Fanutti C, Gidley MJ, Reid JS. Action of a pure xyloglucan endo-transglycosylase (formerly called xyloglucan-specific endo-(1-->4)-beta-D-glucanase) from the cotyledons of germinated nasturtium seeds. Plant J. 1993 May;3(5):691–700. [PubMed]
  • Fry SC, Smith RC, Renwick KF, Martin DJ, Hodge SK, Matthews KJ. Xyloglucan endotransglycosylase, a new wall-loosening enzyme activity from plants. Biochem J. 1992 Mar 15;282(Pt 3):821–828. [PMC free article] [PubMed]
  • Kovatz S, Rathaus M, Aderet NB, Bernheim J. Increased renal prostaglandins in normal pregnancy and in pregnancy with hypertension. Nephron. 1982;32(3):239–243. [PubMed]
  • Konieczny A, Ausubel FM. A procedure for mapping Arabidopsis mutations using co-dominant ecotype-specific PCR-based markers. Plant J. 1993 Aug;4(2):403–410. [PubMed]
  • Li J, Nagpal P, Vitart V, McMorris TC, Chory J. A role for brassinosteroids in light-dependent development of Arabidopsis. Science. 1996 Apr 19;272(5260):398–401. [PubMed]
  • Lincoln C, Britton JH, Estelle M. Growth and development of the axr1 mutants of Arabidopsis. Plant Cell. 1990 Nov;2(11):1071–1080. [PMC free article] [PubMed]
  • Mangelsdorf DJ, Thummel C, Beato M, Herrlich P, Schütz G, Umesono K, Blumberg B, Kastner P, Mark M, Chambon P, et al. The nuclear receptor superfamily: the second decade. Cell. 1995 Dec 15;83(6):835–839. [PubMed]
  • Nishitani K, Tominaga R. Endo-xyloglucan transferase, a novel class of glycosyltransferase that catalyzes transfer of a segment of xyloglucan molecule to another xyloglucan molecule. J Biol Chem. 1992 Oct 15;267(29):21058–21064. [PubMed]
  • Pickett FB, Wilson AK, Estelle M. The aux1 Mutation of Arabidopsis Confers Both Auxin and Ethylene Resistance. Plant Physiol. 1990 Nov;94(3):1462–1466. [PMC free article] [PubMed]
  • Schmidt R, West J, Love K, Lenehan Z, Lister C, Thompson H, Bouchez D, Dean C. Physical map and organization of Arabidopsis thaliana chromosome 4. Science. 1995 Oct 20;270(5235):480–483. [PubMed]
  • Smith RC, Fry SC. Endotransglycosylation of xyloglucans in plant cell suspension cultures. Biochem J. 1991 Oct 15;279(Pt 2):529–535. [PMC free article] [PubMed]
  • Su W, Howell SH. A Single Genetic Locus, Ckr1, Defines Arabidopsis Mutants in which Root Growth Is Resistant to Low Concentrations of Cytokinin. Plant Physiol. 1992 Aug;99(4):1569–1574. [PMC free article] [PubMed]
  • Sun Tp, Goodman HM, Ausubel FM. Cloning the Arabidopsis GA1 Locus by Genomic Subtraction. Plant Cell. 1992 Feb;4(2):119–128. [PMC free article] [PubMed]
  • Sun TP, Kamiya Y. The Arabidopsis GA1 locus encodes the cyclase ent-kaurene synthetase A of gibberellin biosynthesis. Plant Cell. 1994 Oct;6(10):1509–1518. [PMC free article] [PubMed]
  • Wilson AK, Pickett FB, Turner JC, Estelle M. A dominant mutation in Arabidopsis confers resistance to auxin, ethylene and abscisic acid. Mol Gen Genet. 1990 Jul;222(2-3):377–383. [PubMed]
  • Xu W, Purugganan MM, Polisensky DH, Antosiewicz DM, Fry SC, Braam J. Arabidopsis TCH4, regulated by hormones and the environment, encodes a xyloglucan endotransglycosylase. Plant Cell. 1995 Oct;7(10):1555–1567. [PMC free article] [PubMed]
  • Zurek DM, Clouse SD. Molecular cloning and characterization of a brassinosteroid-regulated gene from elongating soybean (Glycine max L.) epicotyls. Plant Physiol. 1994 Jan;104(1):161–170. [PMC free article] [PubMed]

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