• We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
Logo of plntphysLink to Publisher's site
Plant Physiol. May 1996; 111(1): 223–226.
PMCID: PMC157829

Functional expression of the extraplastidial Arabidopsis thaliana oleate desaturase gene (FAD2) in Saccharomyces cerevisiae.

Abstract

The functional expression in yeast of the Arabidopsis thaliana FAD2 gene, encoding the extraplastidial oleate desaturase (1-acyl-2-oleoyl-sn-glycero-3-phosphocholine delta 12-desaturase) is reported. Dienoic fatty acids constituted up to 11% (w/w) of the total fatty acids in transformed Saccharomyces cerevisiae cells and were confirmed to be linoleic acid and delta 9, delta 12-hexadecadienoic acid by gas chromatography-mass spectrometry.

Full Text

The Full Text of this article is available as a PDF (339K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Avelange-Macherel MH, Macherel D, Wada H, Murata N. Site-directed mutagenesis of histidine residues in the delta 12 acyl-lipid desaturase of Synechocystis. FEBS Lett. 1995 Mar 13;361(1):111–114. [PubMed]
  • Elledge SJ, Mulligan JT, Ramer SW, Spottswood M, Davis RW. Lambda YES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations. Proc Natl Acad Sci U S A. 1991 Mar 1;88(5):1731–1735. [PMC free article] [PubMed]
  • Fox BG, Shanklin J, Ai J, Loehr TM, Sanders-Loehr J. Resonance Raman evidence for an Fe-O-Fe center in stearoyl-ACP desaturase. Primary sequence identity with other diiron-oxo proteins. Biochemistry. 1994 Nov 1;33(43):12776–12786. [PubMed]
  • Fox BG, Shanklin J, Somerville C, Münck E. Stearoyl-acyl carrier protein delta 9 desaturase from Ricinus communis is a diiron-oxo protein. Proc Natl Acad Sci U S A. 1993 Mar 15;90(6):2486–2490. [PMC free article] [PubMed]
  • Gietz D, St Jean A, Woods RA, Schiestl RH. Improved method for high efficiency transformation of intact yeast cells. Nucleic Acids Res. 1992 Mar 25;20(6):1425–1425. [PMC free article] [PubMed]
  • Kearns EV, Hugly S, Somerville CR. The role of cytochrome b5 in delta 12 desaturation of oleic acid by microsomes of safflower (Carthamus tinctorius L.). Arch Biochem Biophys. 1991 Feb 1;284(2):431–436. [PubMed]
  • Murphy DJ. Biogenesis, function, and biotechnology of plant storage lipids. Prog Lipid Res. 1994;33(1-2):71–85. [PubMed]
  • Okuley J, Lightner J, Feldmann K, Yadav N, Lark E, Browse J. Arabidopsis FAD2 gene encodes the enzyme that is essential for polyunsaturated lipid synthesis. Plant Cell. 1994 Jan;6(1):147–158. [PMC free article] [PubMed]
  • Shanklin J, Whittle E, Fox BG. Eight histidine residues are catalytically essential in a membrane-associated iron enzyme, stearoyl-CoA desaturase, and are conserved in alkane hydroxylase and xylene monooxygenase. Biochemistry. 1994 Nov 1;33(43):12787–12794. [PubMed]
  • Smith MA, Cross AR, Jones OT, Griffiths WT, Stymne S, Stobart K. Electron-transport components of the 1-acyl-2-oleoyl-sn-glycero-3-phosphocholine delta 12-desaturase (delta 12-desaturase) in microsomal preparations from developing safflower (Carthamus tinctorius L.) cotyledons. Biochem J. 1990 Nov 15;272(1):23–29. [PMC free article] [PubMed]
  • Taylor DC, Barton DL, Rioux KP, Mackenzie SL, Reed DW, Underhill EW, Pomeroy MK, Weber N. Biosynthesis of Acyl Lipids Containing Very-Long Chain Fatty Acids in Microspore-Derived and Zygotic Embryos of Brassica napus L. cv Reston. Plant Physiol. 1992 Aug;99(4):1609–1618. [PMC free article] [PubMed]
  • van de Loo FJ, Broun P, Turner S, Somerville C. An oleate 12-hydroxylase from Ricinus communis L. is a fatty acyl desaturase homolog. Proc Natl Acad Sci U S A. 1995 Jul 18;92(15):6743–6747. [PMC free article] [PubMed]

Articles from Plant Physiology are provided here courtesy of American Society of Plant Biologists

Formats:

Related citations in PubMed

See reviews...See all...

Cited by other articles in PMC

See all...

Links

  • Gene
    Gene
    Gene links
  • GEO Profiles
    GEO Profiles
    Related GEO records
  • HomoloGene
    HomoloGene
    HomoloGene links
  • MedGen
    MedGen
    Related information in MedGen
  • Pathways + GO
    Pathways + GO
    Pathways, annotations and biological systems (BioSystems) that cite the current article.
  • PubMed
    PubMed
    PubMed citations for these articles
  • Taxonomy
    Taxonomy
    Related taxonomy entry
  • Taxonomy Tree
    Taxonomy Tree

Recent Activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...